Skip to main navigation menu Skip to main content Skip to site footer
ASPHC Baner

Vol. 20 No. 5 (2021)

Articles

Identification of fungi inhabiting underground plant parts of soybean [Glycine max (L.) Merrill] in two developmental stages

DOI: https://doi.org/10.24326/asphc.2021.5.13
Submitted: May 19, 2020
Published: 2021-10-29

Abstract

Soybean [Glycine max (L.) Merrill] plants are potential hosts for different pathogens. Soil-borne diseases, caused by pathogenic fungi, are considered to be the main reason for the loss of soybean yields worldwide. The aim of the research was to isolate fungi inhabiting underground parts of soybean in order to identify potential pathogens present in south-east Poland. Research material comprised of seven soybean cultivars grown in field in 2017-2019. Samples collected in three subsequent vegetative seasons constituted soybean plants with disease symptoms on cotyledons, stems and roots. Fragments of the infected plant tissues were subjected to mycological analysis. A total of 1692 pure fungal isolates were obtained from the sampled plants and almost 80% of these isolates were assigned to Fusarium genus. Among Fusarium spp. isolates, the most frequently detected species was F. oxysporum (71.3%). Other detected Fusarium species included: F. graminearum, F. culmorum, F. avenaceum, F. poae, F. solani (syn. Neocosmospora solani), F. sporotrichioides and F. fujikuroi. Other fungi accounted for approx. 19% of the obtained isolates and their main representatives were: Alternaria alternata, Trichoderma sp., Rhizoctonia solani, Mucor sp., Cladosporium sp. and Rhizopus sp. Comparison of these results with published data from other regions of Poland shows differences in prevalence of different species of soil-borne fungi.

References

  1. Agarwal, D.K., Billore, S.D., Sharma, A.N., Dupare, B.U., Srivastava, S.K. (2013). Soybean: introduction, improvement, and utilization in India – problems and prospects. Agric. Res., 2(4), 293–300. https://doi.org/10.1007/s40003-013-0088-0
  2. Arif, M., Chawla, S., Zaidi, N.W., Rayar, J.K., Variar, M., Singh, U.S. (2012). Development of specific primers of genus Fusarium and F. solani using rDNA sub-unit and transcription elongation factor (TEF-1a) gene. Afr. J. Biotechnol., 11(2), 444–447. https://doi.org/10.5897/AJB10.489
  3. Armstrong, G.M., Armstrong, J.K. (1950). Biological races of the Fusarium causing wilt of cowpea and soybean. Phytopathology, 40, 181–193.
  4. Barnett, H.L., Hunter, B.B., (1998). Illustrated genera of imperfect fungi. APS PRESS.
  5. Broders, K.D., Lipps, P.E., Paul, P.A., Dorrance, A.E. (2007). Evaluation of Fusarium graminearum associated with corn and soybean seed and seedlings in Ohio. Plant Dis., 91(9), 1155–1160. https://doi.org/10.1094/PDIS-91-9-1155
  6. Diaz Arias, M.M., Leandro, L.F., Munkvold, G.P. (2013a). Aggressiveness of Fusarium species and impact of root infection on growth and yield of soybeans. Phytopathology, 103, 822–832. https://doi.org/10.1094/PHYTO-08-12-0207-R
  7. Diaz Arias, M.M., Munkvold, G.P., Ellis, M.L., Leandro, L.F. (2013b). Distribution and frequency of Fusarium spp. on soybean roots in Iowa. Plant Dis., 97, 1557–1562. https://doi.org/10.1094/PDIS-11-12-1959-RE
  8. Doyle, J.J., Doyle, J.L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull., 19, 11–15.
  9. Food and Agriculture Organization of the United Nations. FAOSTAT Statistical Database. Rome. (2012, 2016, 2017).
  10. Guzeler, N., Yildirim, C. (2016). The utilization and processing of soybean and soybean products. J. Agric. Fac. Uludag Univ., 30, 546–553.
  11. Gordon, T.R., Martyn, R.D. (1997). The evolutionary biology of Fusarium oxysporum. Annu. Rev. Phytopathol., 35, 111–128.
  12. Hartman, G.L., Rupe, J.C, Sikora, E.J., Domier, L.L., Davis, J.A., Steffey, K.L., (2015). Compendium of soybean disease and pests. 5th ed. American Phytopathological Society, St. Paul, MN, ss. 201.
  13. Horoszkiewicz-Janka, J., Jajor, M., Korbas, M., (2013). Potential risk of infection of pathogenic fungi to legumes (Fabales) and possibilities of their control. Prog. Plant. Prot., 53(4), 762–767.
  14. Hymowitz, T., (1990). Soybeans: the success story. In: Advances in new crops, Janick J., Simon J.E. (ed.). Timber Press, Portland, OR, 159–163.
  15. Jarecki, W., Buczek J., Jańczak-Pieniążek M. (2019). Soybean (Glycine Max (L.) Merr) response to commercial inoculation with Bradyrhizobium japonicum. Appl. Ecol. Environ. Res., 18(5), 6713–6724. https://dx.doi.org/10.15666/aeer/1805_67136724
  16. Jasnić, S.M., Vidić, M.B., Bagi, F.F., Dordević, V.B. (2005). Pathogenicity of Fusarium species in soybean. Proc. Nat. Sci., Matica Serpska Novi Sad, 109, 113–121.
  17. Jendoubi, W., Bouhadida, M., Boukteb, A., Beji, M., Kharrat, M. (2017). Fusarium Wilt affecting chickpea crop. Agriculture, 7(3), 23. https://doi.org/10.3390/agriculture7030023
  18. Jensen, E.S., Peoples, M.B., Hauggaard-Nielsen, H. (2010). Faba bean in cropping systems. Field Crops Res., 115, 203–216. https://doi.org/10.1016/j.fcr.2009.10.008
  19. Jurado, M., Vazquez, C., Patino, B., Gonzalez-Jaen, M.T. (2005). PCR detection assays for the trichothecene-producing species Fusarium graminearum, Fusarium culmorum, Fusarium poae, Fusarium equiseti, and Fusarium sporotrichioides. Syst. Appl. Microbiol., 28, 562–568. https://doi.org/10.1016/j.syapm.2005.02.003
  20. Killebrew, J.F. (1993). Fusaria and other fungi on soybean seedlings and roots of older plants and interrelationships among fungi, symptoms and soil characteristic. Can. J. Plant Pathol., 15, 139–146. https://doi.org/10.1080/07060669309500814
  21. Konstantinova, P., Bonants, P., van Gent-Pelzer, M., van der Zouwen, P., van den Bulk, R. (2001). Development of specific primers for detection and identification of Alternaria spp. In carrot material by PCR and comparison with blotter and plating assays. Mycol. Res., 106 (1), 23–33. https://doi.org/10.1017/50953756201005160
  22. Kopke, U., Nemecek, T. (2010). Ecological services of faba bean. Field Crops Res., 115, 217–233. https://doi.org/10.1016/j.fcr.2009.10.012
  23. Kraft, J.M., Haware, M.P., Hussein, M.M. (1988). Root rot and wilt disease of food legumes. In: World crops: cool season food legumes, Summerfield, R.J. (ed.). Kluwer Academic Press, Boston, 565–575.
  24. Kulik, T., Fordoński, G., Pszczółkowska, A., Płodzień, M., Łapiński, M. (2004). Development of PCR assay based on ITS2 rDNA polymorphism for the detection and differentiation of Fusarium sporotrichioides. FEMS Microbiol. Lett., 239, 181–186. https://doi.org/10.1016/j.femsle.2004.08.037
  25. Leslie, J.F., Pearson, C.A.S., Nelson, P.E., Toussoun, T.A. (1990). Fusarium spp. from corn, sorghum, and soybean in field in the central and eastern United States. Phytopathology, 80(4), 343–50.
  26. Leslie, J.F., Summerell, B.A. (2006). The Fusarium laboratory manual. Blackwell Professional Publishing, Ames, IO.
  27. Mishra, P.K., Fox, R.T.V., Culham, A. (2003). Development of a PCR-based assay for rapid and reliable identification of pathogenic Fusaria. FEMS Microbiol. Lett., 218, 329–332. https://doi.org/10.1111/j.1574-6968.2003.tb.11537.x
  28. Mule, A., Susca, G., Stea, G., Moretti, A. (2004). A species-specific PCR assay based on the calmodulin partial gene for identification of Fusarium verticillioides, F. proliferatum and F. subglutinans. Eur. J. Plant Pathol., 110, 495–502. https://doi.org/10.1023/B:EJPP.0000032389.84048.71
  29. Nicholson, P., Simpson, D.R., Weston, G., Rezanoor, H.N., Less, A.K., Parry, D.W. (1998). Detection and quantification of Fusarium culmorum and Fusarium graminearum in cereals using PCR assays. Physiol. Mol. Plant P., 53, 17–37. https://doi.org/10.1006/pmpp.1998.0170
  30. Parry, D.W., Nicholson, P. (1996). Development of PCR assay to detect Fusarium poae in wheat. Plant Pathol., 45, 383–391. https://doi.org/10.1046/j.1365-3059.1996.d01-133.x
  31. Pastucha, A. (1998). Fungi infecting soybean roots [Glycine max (L.) Merrill] cultivated in the region of Lublin. Veg. Crop. Res. Bull., 49, 81–95.
  32. Patkowska, E. (2001). Grzyby chorobotwórcze porażające korzenie i podstawę łodygi soi [Glycine max (L.) Merrill)], [Pathogenic fungi infesting the roots and base of soybean stems (Glycine max (L.) Merrill)]. Acta Agrobot., 54(1), 105–113. https://doi.org/10.5586/aa.2001.010
  33. Pouralibaba H.R., Rubiales D., Fondevilla S. (2016) Identification of pathotypes in Fusarium oxysporum f.sp. lentis. Eur. J. Plant Pathol., 144, 539–549. https://doi.org/10.1007/s10658-015-0793-6
  34. Sinclair, J.B., Backman, P.A. (1989). Compendium of soybean diseases. APS Press, St. Paul, MN, pp. 106.
  35. Stagnari, F., Mggio, A., Galieni, A., Pisante, A.M. (2017). Multiple benefits of legumes for agriculture sustainability: an overview. Chem. Biol. Techn. Agric., 4(2), 1–13. https://doi.org/10.1186/s40538-016-0085-1
  36. SoyStats.com/international-world-soybean-production/
  37. Turner, A.S., Lees, A.K., Rezanoor, H.N., Nicholson, P. (1998). Refinement of PCR-detection of Fusarium avenaceum and evidence from DNA marker studies for phenetic relatedness to Fusarium tricinctum. Plant Pathol., 47, 278–288. https://doi.org/10.1046/j.1365-3059.1998.00250.x
  38. WWF. (2014). The growth of soy. Impacts and solutions. WWF International Gland, Switzerland.

Downloads

Download data is not yet available.

Most read articles by the same author(s)

1 2 3 > >> 

Similar Articles

<< < 34 35 36 37 38 39 40 41 42 43 > >> 

You may also start an advanced similarity search for this article.