TY - JOUR AU - Marcinek, Barbara AU - Parzymies, Marzena AU - Poniewozik, Monika AU - Kozak, Danuta AU - Durlak, Wojciech PY - 2019/10/28 Y2 - 2024/03/29 TI - THE INFLUENCE OF GROWTH REGULATORS ON DAHLIA PROPAGATION IN TISSUE CULTURE AND ACCLIMATIZATION OF PLANTS IN ex vitro CONDITIONS JF - Acta Scientiarum Polonorum Hortorum Cultus JA - Acta Sci. Pol. Hortorum Cultus VL - 18 IS - 5 SE - Articles DO - 10.24326/asphc.2019.5.5 UR - https://czasopisma.up.lublin.pl/index.php/asphc/article/view/1237 SP - 49-61 AB - <p>The aim of work was to evaluate the influence of cytokinins on dahlia propagated <em>in vitro</em> and their consequent effect on acclimatization. Plant material were shoot tips and nodes. From three cytokinins, benzyladenine, kinetin and 2-isopentenyl-adenine, only BA effectively stimulated shoot multiplication from axiliary buds. The highest multiplication rate was obtained from nodes in presence of 0.25–0.5 mg·dm<sup>–3 </sup>BA. Higher concentrations shortened internodes and decreased leaf blades and growth of callus. 1 mg·dm<sup>–3</sup> of KIN and 2iP positively influenced shoots growth and size of leaves. Gibberellic acid (GA<sub>3</sub>) used with BA increased the number of axillary shoots. The best quality shoots and the highest multiplication rate were obtained when 2 mg·dm<sup>–3</sup> BA was used with 5 mg·dm<sup>–3 </sup>GA<sub>3</sub>. Cytokinins affected rooting and acclimatization <em>ex vitro</em>. Dahlias shoots multiplicated in presence of 1 mg·dm<sup>–3</sup> KIN or 2iP rooted faster in the soil and 100% survived in field, while those from 1 mg·dm<sup>–3</sup> BA media rooted slowly, had shorter shoots and only 60% survived. Plants bloomed after 11–12 weeks in the field. Dahlia plants that had been multiplicated in presence of KIN had a bigger diameter and fresh weight in the field. BA and 2iP positively influenced flowers diameter, length of flower stalk and a number of first order shoots.</p> ER -