TY - JOUR AU - Xi, Yin-Kai AU - Yang, Min AU - Meng, QingHong AU - Huang, Hengyu PY - 2022/04/29 Y2 - 2024/03/28 TI - Induction of adventitious buds and establishment of an efficient regeneration system in Polygonatum cyrtonema Hua JF - Acta Scientiarum Polonorum Hortorum Cultus JA - Acta Sci. Pol. Hortorum Cultus VL - 21 IS - 2 SE - Articles DO - 10.24326/asphc.2022.2.5 UR - https://czasopisma.up.lublin.pl/index.php/asphc/article/view/4228 SP - 53-62 AB - <p><em>Polygonatum cyrtonema</em> Hua is not only a widely used traditional Chinese medicine, but also processed into food and cosmetics. However, its large-scale cultivation and application are limited by the low reproduction rate for traditional artificial propagation technology. Therefore, the aim of this study was to establish an efficient and stable adventitious buds induction system for <em>P. cyrtonema</em> by using one bud rhizome segment as initial explants. The results showed that the optimal medium for the rhizome growth, adventitious buds occurrence and proliferation was MS medium supplemented with 3.0 mg·L<sup>–1</sup> BA, 1.0 mg·L<sup>–1</sup> NAA and 1.0 mg·L<sup>–1</sup> KT, with a proliferation coefficient of 6.5. The best rooting medium was found to be half strength of MS medium with 0.5–1.0 mg·L<sup>–1</sup> NAA. The survival rate was higher than 95%. Finally, an asexual rapid propagation system of <em>P. cyrtonema</em> was established, which can provide the technical basis for the development of nursery culture.</p> ER -