Abstrakt
The purpose of the study was to evaluate the usefulness of primer sequences designed for amplification of mitochondrial genes of Mus musculus to analyze analogous loci in Chinchilla
lanigera's genome. After isolation of DNA from chinchilla's hair and its quantitative and qualitative estimation, the amplification of DNA by the PCR method was carried out. Fragments of 4 genes of mitochondrial genome were under consideration. Universal primers and primers designed on the basis of M. musculus genome were used for amplification of analogous loci of Ch. lanigera's genome. PCR products of 4 examined mtDNA sequences were received. Two of the four products showed homology of flank sequences of DNA individual fragments of both studied species belonging to Rodentia order. PCR optimization, including changes of polymerase, the concentrations of MgCl2 and primers as well as the melting temperature were determined for two of the four DNA sequences.
Bibliografia
CITES, 1991. Appendices I, II, III to the Convention on International Trade in Endangered Species of wild fauna and flora. September 1, 1991. Convention on International Trade In Endangered Species, U.S. Government Printing Office, Washington D.C.
IUCN, 2008. The IUCN Red List of The Threatened Species. International Union for Conservatinon Status of Nature and Natural Resources.
Jimenez J.E., 1996. The extirpation and current status of wild chinchillas (Chinchilla lanigera and Chinchilla brevicaudata). Biol. Cons. 77, 1–6.
Kocher T.D., Thomas W.K., Meyer A., Edwards S.V., Paabo S., Villablanca F.X., Wilson A.C., 1989. Dynamics of mitochondrial DNA evolution in animals: Amplification and sequencing
with conserved primers, PNAS 86, 6196–6200.
Veron J.E.N., 2008. Mass extinctions and ocean acidification: biological constraints on geological dilemmas. Coral Reefs 27, 459–472.
Wang H.Y., Tsai M.P., Tu M.C., Lee S.C., 2000. Universal Primers for Amplification of the Complete Mitochondrial 12S rRNA Gene in Vertebrates. Zool. Stud. 39, 61–66.
Wilson D.E., Reeder D.M., 2005. Mammal species of the world: a taxonomic and geographic reference, 3rd ed. Smithsonian Institution Press, Washington, D.C.
Downloads
Download data is not yet available.
