Abstract
The influence of type and concetration of carbohydrates on in vitro shoot proliferation of Clematis integrifolia was studied. The explants used in the experiment were excised from aseptic cultures incubated on solidifed MS medium. Shoot tips were placed on MS basal medium supplemented with 2 mg 2iP·dm–3 and 0.5 mg IAA·dm–3. The aim of the experiment was to estimate the influence of different carbohydrates: sucrose, galactose or fructose at concentration of 0, 10, 20, 30 or 40 g·dm–3 on growth and proliferation of Clematis integrifolia explants. The obtained results showed that fructose at the concentration of 10 mg·dm–3 had the most positive effect on the growth of the main shoot. The highest proliferation rate was observed on the media with sucrose in concentration of 30 g·dm–3 and the obtained axillary shoots were in the highest amount, length and weight. Galactose in all concentrations used in the experiment definitely inhibited growth and proliferation and rooting of shoots.
References
Amutha S., Ganapathi A., Muruganantham M. 2003. In vitro organogenesis and plant formation in Vigna radiata (L.) Wilczek. Plant Cell Tiss. Org. Cult. 72, 203–207.
Biahoua A., Bonneau L., 1999. Control of in vitro somatic embryogenesis of the spindle tree (Euonymus europaeus L.) by the sugar type and the osmotic potential of the culture medium. Plant Cell Rep. 19, 185–190.
Borkowska B., Szczerba J. 1991. Influence of different carbon sources on invertase-activity and growth of sour cherry (Prunus cerasus L.) shoot cultures. J. Exp. Bot. 42, 911–915.
Debnath S.C. 2005. Effects of carbon source and concentration on development of Lingoberry
(Vaccinium vitis-idaea L.) shoots cultivated in vitro from nodal explants. In Vitro Cell. Dev. Biol.– Plant 41, 145–150.
Enrico R.J., Ramirez S.S., Mroginski L.A., Wall L.G. 2005. In vitro plant regeneration of Alnus acuminata H.B.K. ssp. acuminata and its root nodulation by Frankia. Plant Cell Tiss. Org. Cult. 80, 343–346.
Erwin J.E., Schwarze D., Donahue R. 1997. Factors affecting propagation of Clematis by stem cuttings. HortTechnology 7, 4, 408–410.
Fuentes S.R.L., Calheiros M.B.P., Manetti-Filho J., Vieira L.G.E. 2000. The effects of silver nitrate and different carbohydrate sources on somatic embryogenesis in Coffea canephora. Plant Cell Tiss. Org. Cult. 60, 5–13.
Guan-Kai Y., Li-Zhi J., Li-Jing X., Kuan-Jian, Guan-K.Y., Li Z.J., Kuang J. 2002. A preliminary study on the introduction and cultivation of Clematis. Acta Botanica Yunnanica, 24, 3, 392–396.
Hlebionek G. 1994. Rozmnażanie powojnika górskiego (Clematis montanta ‘Rubens’) za pomocą sadzonek pędowych. Materiały z ogólnopolskiej konferencji „Postęp w rozmnażaniu roślin ozdobnych”. Kraków, 16–17 września 1994, AR im. Hugona Kołłątaja, 77–81.
Kreen S., Svensson M., Rumpunen K. 2002. Rooting of Clematis microshoots and stem cuttings in different substrates. Sci. Hort. 96, 131–357.
Luttman R., Florek F., Preil W. 1994. Silicone-tubing aerated bioreactors for somatic embryo production. Plant Cell Tiss. Org. Cult. 39, 157–170.
Lu M-C. 2002. Micropropagation of Morus latifolia Poilet using axillary buds from mature trees. Sci. Hort. 96, 329–341.
Mandegaran Z., Sieber V. K. 2000. Somatic embryogenesis in Clematis integrifolia × C. viticella. Plant Cell Tiss. Org. Cult. 62, 163–165.
Matthews V. 2002. The International Clematis Register and Checklist. The R.H.S. London, 129.
Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15, 473–497.
Nowak B., Miczyński K., Hudy L. 2004. Sugar uptake and utilisation during adventitious bud differentiation on in vitro leaf explants of ‘Węgierka Zwykła’ plum (Prunus domestica). Plant Cell Tiss. Org. Cult. 76, 255–260.
Petersen K.K., Hansen J., Krogstrup P. 1999. Significance of different carbon sources and sterilization methods on callus induction and plant regeneration of Miscanthus × ogiformis Honda ‘Giganteus’. Plant Cell Tiss. Org. Cult. 58,189–197.
Pritchard J., Wyn-Jones R.G., Tomos A.D. 1991. Turgor, growth and rheological gradients in wheat roots following osmotic stress. J. Exp. Bot. 42, 1043–1049.
Taber R.P., Zhang C., Hu W.S. 1998. Kinetics of Douglas-fir (Pseudotsuga menziesii) somatic embryo development. Can. J. Bot. 76, 863–871.
Wnuk K. 2006. Rozmnażanie tibuchiny wykwintnej (Tibouchina urvilleana/DC./Cogn) in vitro. Praca doktorska, Akademia Rolnicza w Lublinie.
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