The crop rotation studies were carried out in the years 1997–2000 at the Experimental Station Uhrusk on podzolic soil, good rye complex. The experiment was run with the split-block arrangement combined with split-plot one.The analysis was made on potato response expressed with crop yield to soil tillage in crop rotation compared to yield-forming factors of monoculture, under the conditions of full and simplified soil tillage. Potato crop yield obtained in the crop rotation (27.9 t ha-1) proved to be significantly higher as compared to the results recorded in monoculture cultivation of this plant. An increase amounted to 6.6 t ha-1, which constituted about 31%. Full soil tillage as compared to the simplified increased tuber potato crop yield by 1.8 t ha-1; in this case, however, the differences were not significant. The experimental factors, i.e. crop sequence systems and soil tillage methods (full and simplified) did not affect in a significant way the number and mass of the analyzed fractions of potato tubers. Therefore, their percentage in crop is quite close in both, crop rotation and monoculture as well as in the variants with full and simplified soil tillage. The years of the experiment significantly differentiated the total crop yield, number and mass of each fraction, which seems self-evident.