Paper presented the results of studies about the influence of vacuum impregnation of rye grain on its germination energy and ability. The impregnation of the grains was carried out on the vacuum chamber in five different ways. The applied pressure was within the range of 5–100 kPa (atmospheric pressure). The temperature and impregnation time were constant in every case. After impregnation process the germination energy and ability of grains were tested. The statistical test of significance of influence all the factors on germination energy and ability were executed. This test confirmed that the vacuum
impregnation of rye grains hadn’t significant influence on germination energy and ability.