Abstract
With the aim of exposing the harmful influence of Flurenizyd, the studies investigated the ultrastructure and morphology of embryos of loach Misgurnus fossilis L. in early stages of development and larval development on the first days of incubation environment, which contained this antibiotic.. It was shown that the antibiotic at the concentrations of 1 mМ, 5 mМ, 15 mМ caused a high death rate of embryos of loach already on the first day of its application. It was established that Flurenizyd caused structural disturbances of the mitochondria and the cytoplasmic net, in addition to causing an increase of the number of lysosomes in the embryos of loach at the stage of development of 2 blastomeres. The degree of these changes is dose dependent. Flurenizyd at the concentrations of 0.05 mМ, 0.15 mМ and 5 mМ at the stage of development of 64 blasto- meres leads to dystrophic changes. It was shown that the antibiotic at the concentration of 0.05 mМ and 0.15 mМ causes necrotic changes, while at the maximal investigated concentra- tion the influence of Flurenizyd on the structural changes of blastomeres is less visible.
References
Belousov L.W., Dabyahan N.W., Chunayeva М.Z., 1990. Manual to large practical work on em- bryology. Vol. 1, Moscow, 104 (in Russian).
Bodnarchuk N.О., Mandzynets S.М., Petrukh L.І., Sanagurskiy D.І., 2016. Content of primary and secondary products of lipid peroxidation in loach embryos under the effect of Flurenizyd. Studia Biol. 10 (1), 53–60 (in Ukrainian).
Bozkova V.P., Chaylahyan L.М., 1977. Specific role of ions in pre-embryonic development. In: Environment and developing organism. Nauka, Moscow, 210–256 (in Russian).
De Laat S.W., Buwalda R.J., Habets A.M., 1974. Intracellular ionic distribution, cell membrane permeability and membrane potential of the Xenopus egg during first cleavage. Exp. Cell Res. 89, 1–14.
Goida O.А., 1993. Biophysical aspects of early ontogenesis of animals. Кyiv, Sciences thinking, 224 p. (in Ukrainian).
Goida O.А., Oschapovskiy V.V., Sanagurskiy D.I., 1996. New going near the estimation of inter- communication of different parameters influencing on the dynamics of transmembrane poten- tial at the developing of loach embryos. Biophysics 41 (2), 393–399 (in Ukrainian).
Golichenkov V.А., 1996. Methods of embryology researches: Studies. Manual, Moscow, 177 p. (in Russian).
Hinsburg А.S., 1968. Impregnation for fishes and problem of polispermiya. Nauka, Moscow, 368 p. (in Russian).
Kafiani К.А., Malenkov А.G., 1976. Role ion to the homoeostasis of cage in the phenomena of height and development. Successes Modern Biol. 81, 445–463 (in Russian).
Kostomarova А.А., 1975. Loach Misgurnus fossilis. In: Objects of biology of development. Sci- ence, Moscow, 308–323 (in Russian).
Kostyuk P.G., 1978. Research of physical and chemical bases of biological processes. Кyiv, Sci- ences thinking, 20–26 (in Ukrainian).
Kovalyschyn V.І., Fedevych S.V., Brahinets О.G., Kоzаk L.P., Melnyk О.І., Konyk U.V., Nekte- gayev І.О., 2007. Development of trombino- and plazmino- dependent of coagulative dystro- phy ultrastructures of kidney bark at the terms of experiment. Exp. Clin. Physiol. Biochem. 3, 45–58 (in Ukrainian).
Mandzynets S.М., Kulachkovsky О.R., Bura М.V., 2011. Changes of ultrastructure organization of cages of loach embryos are at the terms of influence of avermektyn. Cytol. Genet. 5, 58–64 (in Ukrainian).
Mihalik О., 2013. Modern drugs for chemotherapy of viral infections: a guide. Lviv, 180 p. (in Ukrainian).
Neyfah A., Timofeeva M., 1978. Problems in molecular biology regulation development. Nauka, Moscow, 336 p. (in Russian).
Petrukh L., 1997. Flurenizyd veterinary practice: Coll. materials of international scientific- practical conference “Modern problems of veterinary medicine, zooengineering animal prod- ucts and technologies”, 216–217 (in Ukrainian).
Petrukh L., 2003a. The urgency of the creation and implementation of industrial production of new medicines. Collection descriptions of inventions, 198, Lviv (in Ukrainian).
Petrukh L., 2003b. Urgency creation and implementation in the industrial production of new drugs. Collection of descriptions of inventions, 196, Lviv (In Ukrainian).
Petrukh L., 2008. Fluorene as tuberkulostatyky. Flurenizyd: microbiological, pharmacological and clinical aspects, 464–469 (in Ukrainian).
Petrukh L., 2011. Рharmaceutical education and language. The achievements of pharmaceutical research, 152 (in Ukrainian).
Sanagurskiy D.I., 2008. Biophysics objects. Lviv. Publishing House of Ivan Franko LNU, 522 p. (in Ukrainian).
Tupper J., Sauders J., Elwards Ch., 1970. The onset of electrical communication between cells in the developing starfish embryo. J. Cell Biol. 46 (1), 187–191.
Yaremkevych О., Bura М., Mandzynets S. and others, 2009. Changes dynamics of membrane potential of embryos of loach under act of tiosulfanat. Announcer of national university Lviv Politechnic: Chemistry, technology of substances and their application 644, 78–85 (in Ukrainian).
Zyn А., Bezkorovayny А., Harasym N., Kulachkovsky О., Sanagurskiy D., 2014. Morphological and ultrastructural changes of loach embryos during embryogenesis under the influence of sodium hypochlorite. Announcer of the Lviv University. Series are biological. Producing 67, 18–28 (in Ukrainian).
Downloads
Download data is not yet available.
