The aim of the studies was to determine the effect of different forms of sulphur fertilization on the changes in sulphur sulphate content in soil. The research was based on a three-year-long pot experiment conducted on soil material received from an arable layer of grey brown podsolic soil of silt loam texture. This soil was characterized by low content of sulphur sulphate. The experiment involved one variable factor (sulphur compounds: without S, UAN with sulphur, (NH4)2SO4, K2SO4, Na2SO4, S, CaSO4 2H2O, H2SO4) on eight levels. The tested plants in the 1st year of experiment were spring wheat and white mustard. In the 2nd year spring rape and spring barley were cultivated, and in the 3rd year – spring barley and orchard grass. The paper makes presents this research and discusses the influence of experimental factors on sulphur sulphate content in soil after 1st and 2nd years of the experiment. The experimental treatments and species of cultivated plants clearly differentiate the amounts of sulphur sulphate in soil. The highest content of SO4-S was stated in the soil analyzed after spring wheat and barley harvesting. These plants were characterized by lower nutritional requirements in relation to sulphur in comparison with cruciferae plants. After harvesting of cruciferae plants the content of sulphur sulphate clearly decreased and in many objects was lower in comparison to the amount stated in the soil before the experiment. Out of the applied sulphur fertilization compounds the most of profitable, in case of most plants, were those that used fertilization with (NH4)2SO4, K2SO4 and Na2SO4.