Skip to main navigation menu Skip to main content Skip to site footer

Vol. 15 No. 2 (2016)

Articles

PARTIAL CHARACTERIZATION OF Cherry leaf roll virus (CLRV) ISOLATES INFECTING Sambucus spp. PLANTS IN POLAND

Submitted: October 27, 2020
Published: 2016-04-30

Abstract

Sambucus nigra, S. kamtschatica and S. racemosa plants growing in natural habitats or commercial nurseries in Poland, showing symptoms of vein clearing or
chlorotic patterns were found to be naturally infected with Cherry leaf roll virus (CLRV). Nine virus isolates were characterized by enzyme-linked immunosorbent assay (ELISA) and sequence analysis of RNA2 fragments. Serological test using ELISA kits raised against elderberry, birch, cherry and ash isolates of CLRV showed that tested virus isolates reacted with all polyclonal antibodies used in the experiment. Genetic analysis of 3’non-coding region fragments (3’NCR) of isolates originating from elderberries revealed high level of their sequence identity (more than 95%). All tested isolates clustered exclusively within phylogenetic group E of CLRV. Analysis of polyprotein open reading frame (P2 ORF) fragments showed higher sequence variability, with nucleotide identity ranging from 88 to 93%. This indicates that analysis of P2 ORF fragments may be more suitable for studying CLRV population diversity than analysis of 3’NCR region. Phylogenetic analysis of CP gene sequences confirmed clustering of tested isolates in monotypic group. Overall, the serological and phylogenetic data suggest a host-specific nature of CLRV
variants infecting Sambucus spp. plants in Poland.

References

Berniak, H., Kamińska, M. (2010). Viral diseases of Sambucus plants. Zesz. Probl. Post. Nauk Roln., 554, 15–20.
Boom, R., Sol, C.J.A., Salimans, M.M.M., Jansen, C.L., Wertheim-van Dillen, P.M.E., van der Noordaa, J., 1990. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol., 28, 495–503.
Buchhop, J., von Bargen, S., Büttner, C. (2009). Differentiation of Cherry leaf roll virus isolates from various host plants by immunocapture-reverse transcription-polymerase chain reactionrestriction fragment length polymorphism according to phylogenetic relations. J. Virol. Meth., 157, 147–154.
De Zoeten, G.A., Lauritis, J.A., Mircetich, S.M. (1982). Cytopathology and properties of cherry leaf roll virus associated with walnut blackline decline. Phytopathology, 72, 1261–1265.
Jones, A.T. (1973). A comparison of some properties of four strains of Cherry leaf roll virus. Ann. Appl. Biol., 74, 211–217.
Jones, A.T., Murant, A.F. (1971). Serological relationship between cherry leaf roll, elm mosaic and golden elderberry viruses. Ann. Appl. Biol., 69,11–15.
Juergen Hansen, A., Stace-Smith, R. (1971). Properties of a virus isolated from golden elderberry, Sambucus nigra aurea. Phytopathology, 61, 1222–1229.
Malinowski, T. (1996). Silica capture-reverse transcription-polymerase chain reaction (SC-RTPCR): application for the detection of several plant viruses. In: Diagnosis and identification of plant pathogens. Proceedings of 4th International EFPP Symposium, Bonn 1996, 445–448.
Massalski, P.R., Cooper, J.I. (1984). The location of virus-like particles in the malegametophyte of birch, walnut, cherry naturally infected with cherry leaf roll virus andits relevance to verticaltransmission of the virus. Plant Pathol., 33, 255–262.
Rebenstorf, K., Candresse, T., Dulucq, M.J., Büttner, C., Obermeier, C. (2006). Host speciesdependent population structure of apollen-borne plant virus, Cherry leaf roll virus. J. Virol., 80, 2453–2462.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S. (2011). MEGA5: molecular evolutionary genetics using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol., 28, 2731–2739.
Walkey, D.G.A., Stace-Smith, R., Tremaine, J.H. (1973). Serological, physical and chemical properties of strains of cherry leaf roll virus. Phytopathology, 63, 566–571.
Wang, S., Gergerich, R.C., Wickizer, S.L., Kim, K.S. (2002). Localizationof transmissible and nontransmissible viruses in the vector nematode Xiphinema americanum. Phytopathology, 92, 646–653.
Werner, R., Mühlbach, H.P., Büttner, C. (1997). Detection of cherry leaf roll nepovirus (CLRV) in birch, beech and petunia by immunocapture RT-PCR using a conserved primer pair. Eur. J. For. Path., 27, 309–318.

Downloads

Download data is not yet available.

Similar Articles

<< < 20 21 22 23 24 25 

You may also start an advanced similarity search for this article.