Abstrakt
This study was carried out during 2012 in oil rose (Rosa damascena Mill.) production areas in Burdur prov-ince of Turkey. A total of 102 oil rose leaf samples showing virus-like symptoms were collected from 8 dif-ferent locations. All oil rose leaf samples were tested for Prunus necrotic ringspot virus (PNRSV) using commercially available DAS-ELISA kit with negative and positive control. 16 samples of 102 were found to be infected with PNRSV (16%). DAS-ELISA positive samples were later inoculated onto Cucumis sativus L. cv. Cemre F1, Chenopodium quinoa Wild. and Catharanthus roseus L.G. Don. Inoculation with extracts from PNRSV-positive plants produced chlorotic local lesions on Chenopodium quinoa Wild. The symptoms was not observed on Cucumis sativus L. cv. Cemre F1 and Catharanthus roseus L.G. Don. Total RNA was extracted from leaves of oil rose samples which were positive in the DAS-ELISA and PNRSV- inoculated Chenopodium quinoa Wild. plants. Total RNA was isolated from EZ-10 spin column plant total RNA minipreps kit (Bio Basic, Canada Inc). Reverse Transcription-Polymerase Chain Reaction (RT-PCR) was performed using PNRSV specific primers as described by Rosner (15). The expected frag-ment size of 785 bp was observed after electrophoresis of PCR products in 1% agarose gel. RNA isolated from healthy Chenopodium quinoa Wild. was used as a negative control. No PCR products were amplified from this sample.
Bibliografia
Cairns, T. (2001). The geography and history of the rose. Am. Rose Ann., 18–29.
Cambra, M., Llacer, G., Perez De Sanroman, C. (1982). Use of enzyme-linked immunosorbent assay (ELISA) for virus detection on stone fruit trees in Spain. Acta Hort., 130, 145–150.
Clark, M.F., Adams, A.N. (1977). Characteristics of the microplatemethod of enzyme-linked immunosorbant assay for the detection of plant viruses. J. Gen. Virol., 34, 475–483
Demirözer, O., Kaydan, M.B., Karaca, I., Ben-Dov, Y. (2009). First records of armoured scale insects (Hemiptera: Coc-coidea: Diaspididae) from the oil-rose, Rosa damascena, in Turkey. Hell. Plant Prot. J., 2, 33–35.
Erdiller, G., Elibüyük, Ö., Akbaş, B. (1995). Virus diseases of roses. In: VII. Congress of Phytopathology, Adana, Tur-key, 26–29 September, 286–289.
Ginova, A., Tsvetkov, I., Kondakova, V. (2012). Rosa dama-scena Mill. – an overview for evaluation of propagation methods. Bulgarian J. Agri. Sci., 18, 545–556.
Golino, D.A., Sim, S.T. Cunningham, M. Rowhani, A. (2005). Transmission of rose mosaic viruses. Acta Hort., 751, 217–224.
Manners, M.M. (1997). Effects of rose mosaic disease on performance of hybrid tea roses in Florida. Proc. Florida State Hort. Soc., 110, 118–121.
Moran, J.R., Faragher, J.D., Baker, D.M. (1988). The effect of Prunus necrotic ringspot virus on production and quality of rose flowers. Acta Hort., 234, 429–434.
Moury, B., Cardin, L., Onesto, J.P., Candresse, T., Poupet, A. (2001). Survey of Prunus necrotic ringspot virus in rose and its variability in rose and Prunus spp. Phytopathology, 91, 84–91.
Örmeci-Kart, M.Ç., Ikiz, M., Demircan, V. (2012). Develop-ment of oil rose (Rosa damascena) production and trade in Turkey. Süleyman Demirel Univ. J. Agric. Fac., 7(1), 124–134.
Paduch-Cichal, E., Sala-Rejczak, K. (2011). Biological and molecular characterization of Prunus necrotic ringspot virus isolates from three rose cultivars. Acta Physiol. Plant., 33, 2349–2354.
Rosner, A., Shiboleth, Y., Spiegel, S., Krisbai, L. Kolber, M. (1998). Evaluating the use of immunocapture and sapdilution PCR for the detection of Prunus necrotic ringspot virus. Acta Hort., 472.
Rakhshandehroo, F., Zamani Zadeh, H.R., Modarresi, A. Hajmansoor, S. (2006). Occurrence of Prunus necrotic ringspot virus and Arabis mosaic virus on rose in Iran. Plant Dis., 90, 975.
Sertkaya, G. (2010). An investigation on rose mosaic disease of rose in Hatay-Turkey. In: 21st International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops, Julius-Kühn-Archiv., 427.
Sipahioğlu, H.M., Çağlar, B.K., Baloğlu, S. (2001). Determination of the incidence of PNRSV and ApMV rose viruses in rose by ELISA in the Eastern Mediterranean region. In: Book of Abstracts. IX Congress of Phytopathology, 3–8 September, Tekirdağ, Turkey, 572–577.
Timor, A.N. (2011). World production oil rose and rose oil. E-j. New World Sci. Acad. Nat. Sci., 6(2), 93–110.
Usta, M., Sipahioğlu, H.M., Polat, B. (2005). Comparison of DAS-ELISA and RT-PCR methods for the detection of Prunus necrotic ringspot virus (PNRSV). Yüzüncü Yil Univ. J. Agric. Sci., 15(2), 153–158.
Verma, N., Halan, V., Ram, R., Zaidi, A.A. (2002). Detection of Prunus necrotic ringspot virus in begonia by RT-PCR. New Dis. Rep., 5, 7.
Yardimc, N., Çulal, H. (2009). Occurrence and incidence of Prunus necrotic ringspot virus, Arabis mosaic virus, and Apple mosaic virus on oil rose (Rosa damascena) in the Lakes region of Turkey. New Zeal. Crop Hort., 37, 95–98.
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