Abstrakt
Grasses are very often infected by endophytic fungi of the Neotyphodium and Epichloë genera. They can produce metabolites toxic to livestock as well as beneficially
affect grasses. Thus, endophytes can be used for plants improvement. These facts stimulated a more detailed investigation aimed at providing more insight into the problem of these endophytes in Poland. The aims of the research were as follows: (i) to determine the levels of colonization of Polish-grown cultivars of perennial ryegrass, meadow fescue, and red fescue with endophytic fungi of the genera Neotyphodium and Epichloë; (ii) to determine the potential hazards associated with the toxins produced by the active associations; (iii) to isolate viable endophytes of the genera Neotyphodium and Epichloë from active associations; and (iv) to determine the levels of antagonistic activity of the obtained endophyte isolates against selected microorganisms, in particular serious grass pathogens,
in vitro. The staining method was used for endophytes detection in grass seed collection. Endophytes were isolated on PDA medium and characterized. Antagonistic activity of N/E isolates and seed extracts were determined in dual culture assays and on microscope slides respectively. Ergovaline and lolitrem B content were assayed. There were 242 samples of seeds of 50 cultivars of grasses collected in Poland, including 124 samples of 20 cultivars of perennial ryegrass, 61 samples of 10 cultivars of meadow fescue, and 57 samples of 20 cultivars of red fescue. Endophytic fungi were only detected in 33 seed samples of 5 perennial ryegrass cultivars and in 15 samples of 2 meadow fescue cultivars. The levels of seed colonization of these species reached maximum 8 and 90%, respectively. No colonization by endophytes was found in seeds of red fescue. The viability of endophytic mycelium in the seeds of perennial ryegrass and meadow fescue was very low. Endophytic
fungi were isolated only from 3 and 4 samples of these grasses respectively. In laboratory conditions, endophytic fungi showed stronger antagonistic properties at 25°C. The highest and most frequent growth inhibition was observed for M. nivale, Rhizoctonia solani, Fusarium avenaceum and F. equiseti. The obligate pathogens B. graminis, P. coronata, and P. graminis ssp. graminicola were not sensitive to water and alcohol extracts of E+ seeds. Isolates Fp28, Fp40, and, to a lesser degree, Fp37, originating from meadow fescue, were characterized by the strongest antagonistic properties. Moreover, they did not produce (Fp28 and Fp37), or produced only small amounts (Fp40) of, ergovaline, which is toxic to animals. After additional tests, they could be used as biological factors for improving the utility value of new grass varieties and their resistance to stress factors.
Bibliografia
Bacon C.W., Battista De J., 1991. Endophytic fungi of grasses. [In:] Handbook of applied mycology. Vol. 1. Soil and plants, D.K. Arora, B. Rai, K.G. Mukerji, G.R. Knudsen (eds). Marcel Dekker, New York, 231–256.
Bush L.P., Fannin F.F., Siegel M.R., Dahlman D.L., Burton H.R., 1993. Chemistry, occurrence and biological effects of saturated pyrrolizidine alkaloids associated with endophyte-grass interactions. Agric. Ecosyst. Environ. 44, 81–102.
Cappelli C., Buonaurio R., 2001. Occurrence of endophytic fungi in grass seeds and plants in Italy. [In:] Proceedings of the 4th International Neotyphodium/Grass Interactions Symposium, V.H. Paul and P.D. Dapprich (eds), Soest (Germany), 27–29 September 2000, Universitat-Gesamthochschule Paderborn, Abtailung Soest, Soest, Germany, 131–137.
Christensen M.J., 1996. Antifungal activity in grasses infected with Acremonium and Epichloë endophytes. Austr. Plant Path. 25(3), 186–191.
Clement S.L., Youssef N.N., Bruehl G.W., Kaiser W.J., Elberson L.R., Bradley V., 2004. Effects of different storage temperatures on grass seed germination and Neotyphodium survival. [In:] Proceedings of the 5th International Symposium on Neotyphodium/Grass Interactions, R. Kallenbach, C. Jr. Rosenkrans, T.R. Lock (eds), May 23–26, 2004, Fayetteville, AR, USA, No 511, 163–165.
Faeth S.H., Sullivan T.J., Hamilton C.E., 2001. What maintains high levels of Neotyphodium endophytes in native grasses? A dissenting view and alternative hypotheses. [In:] Proceedings of the 4th International Neotyphodium/Grass Interactions Symposium, V.H. Paul and P.D. Dapprich (eds), Soest (Germany), 27–29 September 2000, Universitat-Gesamthochschule Paderborn, Abtailung Soest, Soest, Germany, 65–69.
Gallagher R.T., Hawkes A.D., Stewart J.M., 1985. Rapid determination of the neurotoxin lolitrem B in perennial ryegrass by high-performance liquid chromatography with fluorescence detection. J. Chromat. 321, 217–226.
Glenn A.E., Bacon C.W., Price R., Hanlin R.T., 1996. Molecular phylogeny of Acremonium and its taxonomic implications. Mycologia 88, 369–383.
Heeswijck Van R., McDonald G., 1992. Acremonium endophytes in perennial ryegrass and other pasture grasses in Australia and New Zealand. Austr. J. Agric. Res. 43, 1683–1709.
Holzmann-Wirth, A., Dapprich, P.D., Eierdanz, S., Heerz, D., Paul, V.H., 2000. Anti-fungal substances extracted from Neotyphodium endophytes. [In:] Proceedings of the 3rd Conference on Harmful and Beneficial Microorganisms in Grassland, Pastures and Turf, V.H. Paul and P.D. Dapprich (eds), Soest (Germany), 26 September 2000, Universitat-Gesamthochschule Paderborn, Abtailung Soest, Soest, Germany, 65–69.
Hoveland C.S., 2000. Endophytes – research and impact. [In:] Proceedings of the 4th International Neotyphodium/Grass Interactions Symposium, V.H. Paul and P.D. Dapprich (eds). Soest (Germany), 27–29 September 2000, Universitat-Gesamthochschule Paderborn, Abtailung Soest, Soest, Germany, 1–8.
Johnson M.C., Dahlmann D.L., Siegel M.R., Bush L.P., Latch G.C.M., Potter D.A., Varney D.R., 1985. Insect feeding deterrents in endophyte infected tall fescue. Plant Dis. 70, 380–382.
Marcinkowska J., 2010. Oznaczanie rodzajów ważnych organizmów fitopatogenicznych (Fungi, Oomycota, Plasmodiophorida). Wyd. SGGW, Warszawa.
Morgan-Jones, G., Gams W., 1982. Notes on Hyphomycetes. XLI. An endophyte of Festuca arundinacea and the anamorph of Epichloë typhina, new taxa in one of two new sections of Acremonium. Mycotaxon. 15, 311–318.
Moyer J.L., 2004. Humidity reduces tall fescue seed-borne endophyte viability. [In:] Proceedings of the 5th International Symposium on Neotyphodium/Grass Interactions, R. Kallenbach, C. Jr. Rosenkrans, T.R. Lock (eds), May 23–26, 2004, Fayetteville, AR, USA, No 515, 172–174.
Pańka D., Łukanowski A., 2001. Occurence of Acremonium lolii in perennial ryegrass (Lolium perenne L.) cultivated in the Kujawy and Pomerania region of Poland. [In:] Proceedings of the 4th International Neotyphodium/Grass Interactions Symposium, V.H. Paul and P.D. Dapprich (eds). Soest (Germany), 27–29 September 2000, Universitat-Gesamthochschule Paderborn, Abtailung Soest, Soest, Germany, 419–421.
Pańka D., Sadowski Cz., 2002. Occurence of Fungal endophytes in perennial Ryegrass (Lolium perenne L.) cultivars in Poland. [In:] Multi-function Grasslands, Quality Forages, Animal Products and Landscapes, J.L. Durand, J.C. Emile, C. Huyghe, G.Lemaire (eds). Grassland Science in Europe 7, 540–541.
Pańka D., 2008. Occurrence of Neotyphodium lolii and its antifungal properties Phytopathologia Polonica 48, 5–12.
Pfannmöller M., Eggestein S., Schöberlein W., 1994. Endophytes in European varieties of Festuca species. IOBC/WPRS Bulletin 17(1), 105–109.
Porter J.K., Thompson F.N. Jr., 1992. Effects of fescue toxicosis on reproduction in livestock. J. Anim. Sci. 70, 1594–1603.
Prończuk S., Prończuk M., 2000. Nasiennictwo traw dla rekultywacji terenów trudnych. Łąkarstwo w Polsce 3, 129–139 [In Polish].
Riccioni L., Monopoli V., Odoardi M., 1994. Endophytic fungi in tall fescue in Italy. IOBC/WPRS Bulletin 17(1), 127–130.
Rottinghaus G.E., Garner G.B., Cornell C.N., Ellis J.L., 1991. HPLC method for quantitating ergovaline in endophyte-infested tall fescue: Seasonal variation of ergovaline levels in stems with leaf sheaths, leaf blades, and seedheads. J. Agric. Food Chem. 39, 112–115.
Rolston M.P., Hare M.D., Moore K.K., Christensen M.J., 1986. Viability of Lolium endophyte fungus in seed stored at different moisture contents and temperatures. New Zealand J. Exp. Agric. 14, 297–300.
Saha D.C., Jackson M.A., Johnson-Cicalese J.M., 1988. A rapid staining method for detection of endophytic fungi in turf and forage grasses. Phytopathology 78(2), 237–239.
Schardll C.L., 2001. Epicholë festucea and related mutualistic symbionts of grasses. Fungal Genet. Biol. 33, 69–82.
Siegel M.R., 1993. Acremonium endophytes: our current state of knowledge and future directions for research. Agric. Ecosyst. Environ. 44, 301–321.
Siegel M.R., Latch G.C.M., Johnson M.C., 1985. Acremonium fungal endophytes of tall fescue and perennial ryegrass: Significance and control. Plant Dis. 69(2), 179–183.
Smith S.E., Read D.J., 1997. Mycorrhizal symbiosis. Second edition. Academic Press, San Diego, CA.
Thompson F.N., Stuedeman J.A., 1993. Phytophysiology of fescue toxicosis. Agric. Ecosystems Environ. 44, 263–281.
Wäli P.R., Helander M., Nissinen O., Saikkonen K., 2006. Susceptibility of endophyte-infected grasses to winter pathogens (snow molds). Can. J. Bot. 84(7), 1043–1051.
Wheatley W.M., 2005. Endophytes, quality assurance, and the seed trade in eastern Australia. [In:] Neotyphodium in cool-season grasses, C.A. Roberts, C.P. West, D.E. Spiers (eds). Blackwell Publishing, 351–360.
White J.F. Jr., 1994. Taxonomic relationships among the members of the Balansiae (Clavicipitales). [In:] Biotechnology of endophytic fungi of grasses, C.W. Bacon and J.F. White, Jr. (eds). CRC Press, Boca Raton, FL, 3–20.
White Jr, J.F., Cole G.T., 1985. Endophyte host associations in forage grasses III. In vitro inhibition of fungi by Acremonium coenophialum. Mycologia 77, 487–489.
White J.F. Jr., Reddy P.V., 1998. Examination of structure and molecular phylogenetic relationships of some graminicolous symbionts in genera Epichloë and Parepichloë. Mycologia 90, 226–234.
Wiewióra B., Prończuk M., Ostrowska A., 2006. Infekcja nasion traw przez endofity w kolejnych latach użytkowania plantacji. Biul. IHAR 242, 285–293.
Wiewióra B., Prończuk M., Ostrowska A., Żurek G., 2007. Endophyte occurrence in breeding strains of meadow fescue (Festuca pratensis) cv. ‘Pasja’. Phytopathologia Polonica 46, 5–11.
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