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Tom 30 Nr 3 (2012)

Articles

Application of chosen Mus musculus mtDNA gene primers in amplification of Chinchilla lanigera parallel loci

Przesłane: marca 15, 2021
Opublikowane: 2012-09-30

Abstrakt

The purpose of the study was to evaluate the usefulness of primer sequences designed for amplification of mitochondrial genes of Mus musculus to analyze analogous loci in Chinchilla
lanigera's genome. After isolation of DNA from chinchilla's hair and its quantitative and qualitative estimation, the amplification of DNA by the PCR method was carried out. Fragments of 4 genes of mitochondrial genome were under consideration. Universal primers and primers designed on the basis of M. musculus genome were used for amplification of analogous loci of Ch. lanigera's genome. PCR products of 4 examined mtDNA sequences were received. Two of the four products showed homology of flank sequences of DNA individual fragments of both studied species belonging to Rodentia order. PCR optimization, including changes of polymerase, the concentrations of MgCl2 and primers as well as the melting temperature were determined for two of the four DNA sequences.

Bibliografia

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