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Tom 34 Nr 4 (2016)

Articles

Zmiany strukturalne zarodków piskorza podczas embriogenezy przy działaniu flurenizydy

Przesłane: czerwca 26, 2019
Opublikowane: 2016-12-19

Abstrakt

W celu udowodnienia szkodliwego wpływu flurenizydy zbadano ultrastruktury i morfologię zarodków piskorza Misgurnus fossilis L. w początkowych stadiach embriogenezy i larw w pierwszych dniach inkubacji w pożywce, która zawierała roztwór tego antybiotyku. Wy- kazano, że flurenizyda w stężeniu 1 mM, 5 mM, 15 mM powoduje wzrost śmiertelności zarodków piskorza w pierwszym dniu jej stosowania. Stwierdzono, że flurenizyda prowadzi do zaburzeń strukturalnych w mitochondriach i gładkiej siateczce śródplazmatycznej, powoduje wzrost liczby lizosomów w zarodkach piskorza w stadium 2 blastomerów. Stopień tych zmian zależy od dawki preparatu. Flurenizyda w stężeniach 0,05 mM, 0,15 mM i 5 mM na etapie 64 blastomerów prowa- dzi do zmian degeneracyjnych. Wykazano, że w stężeniu 0,05 mM i 0,15 mM powoduje zmiany martwicze, natomiast w maksymalnym stężeniu powoduje mniej wyraźne zmiany strukturalne blastomerów.

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