The research was based on the analysis of plant material taken from pot experiment carried out in the years 1999–2000. The experiment was set up using a complete randomisation method and it ranged over three quantitative experimental factors: sulphur dose, sulphur form, dose and form of lime fertilizers. In the first year of the experiment the test plant was spring wheat and in the second year spring rape. Sulphur was implemented as sodium sulphate and as elementary sulphur. Sulphur fertilization was implemented before the sowing of wheat and rape. Soil liming was made using calcium carbonate and dolomite and it took place once before the experiment was set up. The purpose of this work was to determine the influence of soil supply in sulphur, calcium and magnesium on total sulphur content in the experimental plants. Wheat plants were harvested at the in stages of tillering, flowering and full maturity, while rape was harvested at the stages of plant rosette, flowering and full maturity. The applied factors influenced the changes of percentage of total sulphur content in spring forms of wheat and rape. The content of sulphur was determined by the stage of plant developement, plant organ, kind of sulphur fertilization and liming. Percentage content of total sulphur both in spring forms of wheat and rape was positively connected with the dose of sulphur implemented with fertilizers. Higher growth of sulphur content in plants took place in the series fertilized by sodium sulphate as compared to plants fertilized by the elementary form of sulphur. Soil liming by dolomite and calcium carbonate contributed to a decrease of the percentage content of total sulphur in the analysed forms of wheat and rape.