Abstract
The aim of the study was to induce somaclonal variability in the callus culture of L. hirsutum f. typicum and L. chilense and to characterize them in respect to tolerance to salinity. Callus was initiated on the cotyledon fragments grown on the medium supplemented with NAA and BAP. The tolerance of callus to salt was tested on MS media containing NaCl in concentration 25, 50, 75, 100, 200 mM. Callus tolerant to 100 mM NaCl was next regenerated by ten weeks on the media with different doses of growth regulators. After this time genetic differences between selected fragments and control (MS medium containing no growth regulators) were determining using ISSR-PCR method. The results show that NaCl concentration significantly affects the regeneration of callus in L. hirsutum f. typicum and L. chilense. The dose 200 mM NaCl of the medium results, in both species, in callus dying. Comparing the genetic similarity of examined callus samples with the control ones in both species, it may be stated that the differences in their response to NaCl and applied growth regulators were generally in the range 2–30%.
References
Alaoui-Sossé B., Sehmer L., Bartola P., Dizengremel P., 1998. Effect of NaCl salinity on growth and mineral partitioning in Quercus robur L., a rhythmically growing species. Trees – Struct. and Funct. 12, (7), 424–430.
Ates E., Tekeli A.S., 2007. Salinity tolerance of Persian clover (Trifolium resupinatum var. Majus Boiss) lines at germination and seedling stages. World J. Agr. Sci. 3.1, 71–79.
Borsani O., Cuartero J., Fernandez J.A., Valpuesta V., Botella M., 2001. Identification of two loci in tomato reveals distinct mechanisms for salt tolerance. Plant Cell. 13, 873–888.
Borsani O., ValpuestaV., Botella M.A., 2003. Developing salt-tolerant plants in a new century: a molecular biology approach. Plant Cell, Tiss. Org. Cult. 73, 101–115.
Cano E.A., Perez-Alfocea F., Moreno V., Bolarin M., 1996. Response to NaCl stress of cultivated and wild tomato species and their hybrids in callus cultures. Plant Cell Rep. 15, 791–794.
Domin M., 2003. Usefulness of new varieties of the Italian broccoli to industrial refrigeration. Inżynieria Roln., 7(8), 81–88.
Dziadczyk P., Bolibok H., Tyrka M., Hortyński A., 2003. In vitro selection of strawberry (Fragaria x ananasa Duch.) clones tolerant to salt stress. Euphytica. 132, 49–55.
Enmilio A.C., Perez-Alfocea F., Moreno V., Caro N.M., Bolarin M.C., 1998. Evaluation of salt tolerance in cultivated and wild tomato species through in vitro shoot apex culture. Plant Cell, Tiss. and Org. Cult. 53, 10–26.
Hulisz P., 2007. Wybrane aspekty badań gleb zasolonych w Polsce. Wyd. UMK, Toruń, 76–23.
Jia W., Wang Y., Hang S., Zhang J., 2002. Salt stress induced ABA accumulation is more sensitively triggered in roots than in shoots. J. Exp. Bot. 53, 2201–2206.
Karp A., 1991. On the current understanding of somaclonal variation. Oxford Surveys of Plant Mol. and Cell Biol. 7, 1–58.
Karp B., 1995. Somaclonal variation as a tool for crop improvement. Euphytica 85, 295–302.
Kochieva E.Z., Ryzhova N.N., Khrapalova I.A., Pulchalskyi V.A., 2002. Genetic diversity and phylogenetuc relationship in Genus Lycopersicon (Tourn,) Mill. As revealed by inter-simple sequences repeat (ISSR) analysis. Russian J. Gen. 38, 958–966.
Larkin P.J., Scowcroft W.R., 1983. Somaclonal variation and eyespot toxin tolerance in sugarcane. Plant Cell, Tiss. Org. Cult. 2, 111–122.
Lutts S., Kinet J.M., Bouharmont J., 1998. NaCl in pact on somaclonal variation exhibited by tissue culture-derived fertile plants of rice (Oryza sativa L.). J. Plant Phys. 152, 92–103.
Lu S., Peng X., Guo Z., Zhang G., Wang Z., Wang C., Pang C., Fan Z. Wang J., 2007. In vitro selection of salinity tolerant variants from triploid bermudagrass (Cynodon transvaalensis × C. dasctylon) and their physiological responses to salt and drought stress. Plant Cell Rep. 26, 413–420.
Monforte A.J., Asins M.J., Carbomell E.A., 1997. Salt tolerance in Lycopersicon species. VI. Genotype-by-salinity interaction in quantitative trait loci detection: constitutive and response QTLs. Theor. Appl. Genet. 95, 706–713.
McCoy T.J., 1987. Tissue culture evaluation of NaCl tolerance in Medicago species. Cellurar versus whole plant response. Plant Cell Rep. 6, 31–34.
Peschke V., Philips R., 1992. Genetic implications of somaclonal variation in plants. Adv. i Genet. 30, 41–75.
Pollard J.W., Walker J.M., 1990. Plant Cell and Tissue Culture. Methods in Molecular Biology. Humana Press. Clifton. New Jersey.
Queirós F., Fidalgo F., Santos I., Salema R., 2007. In vitro selection pf salt tolerant cell lines in Solanum tuberosum L. Biologia Plant. 51, 4, 728–734.
Rzepka-Plevneš D., 1990. Studia nad mieszańcami międzygatunkowymi Secale sp. pod kątem przydatności niektórych ich cech w hodowli żyta S. cereale L. (Studies on the interspecific Secale sp. hybrids in respect to the suitability of some of their traits for breeding of rye S. cereale L. Ed. Agricult. Univ. in Szczecin, 3–71.
Rzepka-Plevneš D., Furmanek T., 1997. Reakcja roślin pomidora (Lycopersicon sp.) na stres solny w warunkach in vitro. Mat. Konf. „Zastosowanie kultur in vitro w fizjologii roślin. PAN, Kraków, 197–204.
Rzepka-Plevneš D., Kulpa D., Charkot S., 2006. Salt tolerance screening of Pinus sylvestris L. from the dunes of the south-west coast of the Baltic Sea under in vitro condition. J. Food, Agri. Envir. 4 (2), 329–334.
Rzepka-Plevneš D., Kulpa D., Smolik M., Cłapa K., 2007a. Somaclonal variation in the tomato Lycopersicon peruvianum. W. J. Agri. Sci. 2 (3), 50–56.
Rzepka-Plevneš D., Kulpa D., Smolik M., Główka M., 2007b. Somaclonal variation in tomato L. pennelli and L. peruvianum f. glandulosum characterized in respect to salt tolerance. J. Food Agri. Envir. 5, (2), 194–201.
Wahid A., Rao A., Rasul E., 1997. Identification of salt tolerance traits in sugarcane lines. Field Crops Reas. 54(1), 9–17.
Tanksley S. D., McCouch S. R., 1997 Seed Banks and Molecular Maps: Unlocking Genetic Potential from the Wild. Sci. 277(5329), 1063–1066.
Zhang L.Q., Zheng Y.P., Lup S.Y., Hu J.G., 1997. Salt tolerance of calli of Populus clones of Pinus elliotii in tissue culture. J. Zhejiang For. Coll. 14, 1, 14–21
Zietkiewicz E., Rafalski A., Labuda S.D., 1994. Genomic fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics. 20, 176–183.
Downloads
Download data is not yet available.
