Skip to main navigation menu Skip to main content Skip to site footer
ASPHC Baner

Vol. 22 No. 4 (2023)

Articles

Susceptibility of grapevine cultivars to Eutypa lata in Serbia

DOI: https://doi.org/10.24326/asphc.2023.4489
Submitted: November 29, 2021
Published: 2023-08-31

Abstract

The species Eutypa lata is one of the most critical phytopathogenic fungi that presents a threat to vineyards by causing significant economic damage. This grapevine disease is hazardous because there is no adequate chemical protection, so after its progression, there is a decrease in yield and dieback of parts or whole infected shoots. There are also no grapevine cultivars resistant to this disease. For these reasons, in this paper, testing of the susceptibility of a pair of grapevine cultivars to this disease was carried out. The testing was performed under semicontrolled conditions and included six of the most economically essential grapevine cultivars in Serbia (Riesling italico, Cabernet Sauvignon, Cardinal, Rkaziteli, Pinot noir, and Prokupac). Testing was performed using 14 isolates of E. lata isolated in Serbia and two control isolates obtained from the INRA. For specific detection of isolates of Eutypa spp. included in the experiment, a specific pair of primers, Lata 1/Lata 2.2, was applied, and an amplicon of the expected size of approximately 385 bp was established in all isolates. In the conditions of the set experiment, of all of the domestic and foreign-tested grapevine cultivars, the local cultivar Prokupac showed greater resistance to the isolates. In contrast, the cultivars Riesling italico, Cabernet Sauvignon and Cardinal, Pinot noir showed sensitivity to the isolates of E. lata, but not to the same extent as the cultivar Rkaziteli, which proved to be susceptible.

References

  1. Cardot, C., Mappa, G., La Camera, S., Gaillard, C., Vriet, C., Lecomte, P., Ferrari, G., Coutos-Thévenot., P. (2019). Comparison of the molecular responses of tolerant, susceptible and highly susceptible grapevine cultivars during interaction with the pathogenic fungus Eutypa lata. Front. Plant Sci., 10, 991. https://doi.org/10.3389/fpls.2019.00991 DOI: https://doi.org/10.3389/fpls.2019.00991
  2. Carter, M.V. (1994). Wood and root diseases caused by fungi. Eutypa dieback. In: Compendium of grape diseases, Pearson, R.C., Goheen, A.C. (eds.). APS Press, The American Phytopathological Society, St. Paul, Minnesota, USA, 32–34.
  3. Choi, Y.W., Hyde, K.D., Ho, W.H. (1999). Single spore isolation of fungi. Fungal Divers., 3, 29–38.
  4. Day, J.P., Shattock, R.C. (1997). Aggressiveness and other factors relating to displacement of populations of Phytophthora infestans in England and Wales. Eur. J. Plant Pathol., 103, 379–91. DOI: https://doi.org/10.1023/A:1008630522139
  5. Fontaine, F., Pinto, C., Vallet, J., Clément, C., Gomes, A.C., Spagnolo, A. (2016). The effects of grape vine trunk diseases (GTDs) on vine physiology. Eur. J. Plant Pathol., 144, 707–721. https://doi.org/10.1007/s10658-015-0770-0 DOI: https://doi.org/10.1007/s10658-015-0770-0
  6. Gramaje, D., Úrbez-Torres, J.R., Sosnowski, M.R. (2018). Managing grapevine trunk diseases with respect to etiology and epidemiology: current strategies and future prospects. Plant Dis., 102, 12–39. https://doi.org/10.1094/PDIS-04-17-0512-FE DOI: https://doi.org/10.1094/PDIS-04-17-0512-FE
  7. Hrycan, J., Hart, M., Bowen, P., Forge, T., Úrbez-Torres, J.R. (2020). Grapevine trunk disease fungi: their roles as latent pathogens and stress factors that favour dis-ease development and symptom expression. Phytopathol. Mediterr., 59(3), 395–424. https://doi.org/10.14601/Phyto-11275
  8. Lecomte, P., Péros, J.P., Blancard, D., Bastien, N., Délye, C. (2000). PCR Assays that identify the grapevine dieback fungus Eutypa lata. Appl. Environ. Microbiol., 4475–4480. https://doi.org/10.1128/aem.66.10.4475-4480 DOI: https://doi.org/10.1128/AEM.66.10.4475-4480.2000
  9. Moisy, C., Berger, G. Flutre, T. Lee Cunffandand, L., Péros, J.P. (2017). Quantitative assessment of grape vine wood colonization by the dieback fungus Eutypa lata. J. Fungi, 3(2), 21, 1–15. https://doi.org/10.3390/jof3020021 DOI: https://doi.org/10.3390/jof3020021
  10. Mondello, V., Songy, A., Battiston, E., Pinto, C., Coppin, C., Trotel-Aziz, P., Clément, C., Mugnai, L., Fontaine, F. (2017). Grapevine trunk diseases: a review of fifteen years of trials for their control with chemicals and biocontrol agents. Plant Dis., 102, 1189–1217. https://doi.org/10.1094/PDIS-08-17-1181-FE DOI: https://doi.org/10.1094/PDIS-08-17-1181-FE
  11. Munkvold, G.P., Duthie, J.A., Marois, J.J. (1994). Reductions in yield and vegetative growth of grapevines due to Eutypa dieback. Phytopathology, 84, 186–192. https://doi.org/10.1094/Phyto-84-186 DOI: https://doi.org/10.1094/Phyto-84-186
  12. Pitt, W.M., Trouillas, F.P., Gubler, W.D., Savocchia, S., Sosnowski, M.R. (2013). Pathogenicity of Diatrypaceous fungi on grapevine in Australia. Plant Dis., 97, 749–756. https://doi.org/10.1094/PDIS-10-12-0954-RE DOI: https://doi.org/10.1094/PDIS-10-12-0954-RE
  13. Rolshausen, P.E., Greve, L.C., Labavitch, J.M., Mahoney, N.E., Molyneux, R.J., Gubler, W.D. (2008). Pathogenesis of Eutyapa lata in grapevine: Identification of virulence factors and biochemical characterization of cordon dieback. Phy-topathology, 98, 222–229. https://doi.org/10.1094/PHYTO-98-2-0222 DOI: https://doi.org/10.1094/PHYTO-98-2-0222
  14. Rolshausen, P.E., Baumgartner, K., Travadon, R., Pouzoulet, J. (2014). Identification of Eutypa spp. causing Eutypa dieback of grapevine in Eastern North America. Plant Dis., 98, 483–491. http://dx.doi.org/10.1094/PDIS-08-13-0883-RE DOI: https://doi.org/10.1094/PDIS-08-13-0883-RE
  15. Rolshausen, P.E., Sosnowski, M., Trouillas, F.P. Gubler, W.D. (2015). Diseases caused by fungi and Oomycetes. Eutypa dieback. In: Compendium of grape diseases, disorders, and pests, Wilcox, W.F., Gubler, W.D., Uyemoto, J.K. (eds.). APS Press, The American Phytopathological Society, St. Paul, Minnesota, USA, 57–61.
  16. Sosnowski, M.R., Lardner, R., Wicks, T.J., Scott, E.S. (2007a). The influence of grapevine cultivar and isolate of Eutypa lata on wood and foliar symptoms. Plant Dis., 91, 924–931. https://doi.org/10.1094/PDIS-91-8-0924 DOI: https://doi.org/10.1094/PDIS-91-8-0924
  17. Sosnowski, M.R., Shtienberg, D., Creaser, M.L., Wicks, T.J., Lardner, R., Scott, E.S. (2007b). The influence of climate on foliar symptoms of Eutypa dieback in grapevines. Phytopathology 97, 1284–1289. https://doi.org/10.1094/PHYTO-97-10-1284 DOI: https://doi.org/10.1094/PHYTO-97-10-1284
  18. Sosnowski, M.R., McCarthy, G. (2017). Economic impact of grapevine trunk disease management in Sauvignon Blanc vineyards of New Zealand. Wine Vitic. J., 32, 42–48.
  19. Travadon, R., Rolshausen, P.E., Gubler, W.D., Cadle-Davidson, L., Baumgartner, K. (2013). Susceptibility of cultivated and wild Vitis spp. to wood infection by fungal trunk pathogens. Plant Dis., 97, 1529–1536. https://doi.org/10.1094/PDIS-05-13-0525-RE DOI: https://doi.org/10.1094/PDIS-05-13-0525-RE
  20. Trouillas, F.P., Gubler, W.D. (2010). Host range, biological variation, and phylogenetic diversity of Eutypa lata in California. Phytopathology, 100(10), 1048–1056. https://doi.org/10.1094/PHYTO-02-10-0040 DOI: https://doi.org/10.1094/PHYTO-02-10-0040
  21. Trouillas, F.P., Úrbez-Torres, J.R., Gubler, W.D. (2010). Diversity of diatrypaceous fungi associated with grapevine canker diseases in California. Mycologia, 102(2), 319–336. https://doi.org/10.3852/08-185 DOI: https://doi.org/10.3852/08-185
  22. Úrbez-Torres, J.R., Peduto, F., Striegler, R.K., Urrea-Romero, K.E., Rupe, J.C., Cartwright, R.D, Gubler, W.D. (2012). Characterization of fungal pathogens associated with grapevine trunk diseases in Arkansas and Missouri. J. Fungal Div., 52 (1), 169–189. https://doi.org/10.1007/s13225-011-0110-4 DOI: https://doi.org/10.1007/s13225-011-0110-4
  23. Živković, S., Vasić, T., Anđelković, S., Jevremović D., Trkulja, V. (2012a). Identification and characterization of Eutypa lata on grapevine in Serbia. Plant Dis., 96, 913. https://doi.org/10.1094/PDIS-11-11-0990-PDN DOI: https://doi.org/10.1094/PDIS-11-11-0990-PDN
  24. Živković, S., Vasić, T., Trkulja, V., Krnjaja V., Marković, J. (2012b). Pathogenicity on grapevine and sporulation of Eutypa lata isolates originating from Serbia. Rom. Biotechnol. Lett., 3(17), 7379–7388.
  25. Živković, S. (2019). Characterization of Eutypa lata, causal agent of grapevine dieback in Serbia and susceptibility of varieties. (Dissertation). Faculty of Agriculture. University of Belgrade, 1–171.

Downloads

Download data is not yet available.