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Vol. 21 No. 4 (2022)

Articles

In vitro multiplication of Mentha piperita L. and comparative evaluation of some biochemical compounds in plants regenerated by micropropagation and conventional method

DOI: https://doi.org/10.24326/asphc.2022.4.5
Submitted: December 14, 2021
Published: 2022-08-31

Abstract

The aim of this study was to elaborate an efficient in vitro multiplication protocol for Mentha piperita L. (peppermint) and to perform a comparative evaluation of some biochemical compounds in plants regenerated by micropropagation and conventional method. The use of a plain Murashige and Skoog (MS) basal medium favored the induction of regenerative processes, the percentage of explants that started to grow four weeks after inoculation being 92%. The highest multiplication rate (7.12 shoots/explant) and the highest average shoot length (8.11 cm) were obtained on the MS medium supplemented with 1 mg/L benzylaminopurine, when nodal fragments were used as explants. The rooting phase was not necessary, the shoots developing roots on the multiplication medium. The acclimatization rate of in vitro regenerated plants to ex vitro conditions was 96%. Although biochemical investigations revealed some differences between in vitro regenerated plants and those obtained by conventional methods, the results obtained show that micropropagation can be used successfully to obtain high-quality peppermint biological material, a potential source of bioactive compounds with therapeutic effect.

References

  1. Aliyu, A.B., Ibrahim, M.A., Ibrahim, H., Musa, A.M., Lawal, A.Y., Oshanimi, J.A., Usman, M., Abdulkadir, I.E., Oyewate, A.O., Amupitan, J.O. (2012). Free radical scavenging and total antioxidant capacity of methanol extract of Ethulia conyzoides growing in Nigeria. Rom. Biotech. Lett., 17, 7458–7465.
  2. Fadel, D., Kintzios, S., Economou, S.A., Moschopoulou, G., Constantinidou, H.A. (2010). Effect of different strength of medium on organogenesis, phenolic accumulation and antioxidant activity of spearmint (Mentha spicata L.). Open Hortic. J., 3, 31–35. http://dx.doi.org/10.2174/1874840601003010031 DOI: https://doi.org/10.2174/1874840601003010031
  3. George, E.F. (1993). Plant propagation by tissue culture. Part 1: the technology. The Edington Technology Exegetics Ltd., Westbury.
  4. Ghanti, K., Kaviraj, C.P., Venugopal, R.B., Jabeen, F.T.Z., Rao, S. (2004). Rapid regeneration of Mentha piperita L. from shoot tip and nodal explants. Indian J. Biotechnol., 3, 594–598.
  5. Hirata, T., Murakami, S., Ogihara, K., Suga, T. (1990). Volatile monoterpenoid constituents of the plantlets of Mentha spicata produced by shoot tip culture. Phytochemistry, 29(2), 493–495. https://doi.org/10.1016/0031-9422(90)85103-M DOI: https://doi.org/10.1016/0031-9422(90)85103-M
  6. Holm, G. (1954). Chlorophyll mutations in barley. Acta Agr. Scand., 4(1), 457–471. https://doi.org/10.1080/00015125409439955 DOI: https://doi.org/10.1080/00015125409439955
  7. Jeyakumar, M., Jayabalan, N. (2000). An efficient method for regeneration of plantlets from nodal explants of Psoralea corylifolia Linn. Plant Cell Biotech. Mol. Biol., 1(1–2), 37–40.
  8. de Klerk, G.J., van der Krieken, W., de Jong, J.C. (1999). Reviewthe formation of adventitious roots: new concepts, new possibilities. In Vitro Cell. Dev. Biol. Plant, 35(3), 189–199. http://dx.doi.org/10.1007/s11627-999-0076-z DOI: https://doi.org/10.1007/s11627-999-0076-z
  9. Kukreja, A.K., Dhawan, O.P., Mathur, A.K., Ahuja, P.S., Mandal, S. (1991). Screening and evaluation of agronomically useful somaclonal variations in Japanese mint (Mentha arvensis L.). Euphytica, 53, 183–191. https://doi.org/10.1007/BF00023270 DOI: https://doi.org/10.1007/BF00023270
  10. Maity, S.K., Kundu, A.K., Tiwary, B.K. (2011). Rapid and large scale micropropagation of true to type clone of Mentha arvensis Linn. (Lamiaceae) – a valuable medicinal plant. Indian J. Appl. Pure Biol., 26(2), 193–198.
  11. Manik, S.R., Yatoo, G.M., Ahmad, Z., Nathar, V.N. (2012). Direct organogenesis of Mentha piperita L. from shoot tip, nodal and sucker explants. J. Agric. Tech., 8(2), 663–669.
  12. Matyssek, R., Agerer, R., Ernst, D., Munch, J.-C., Osswald, W., Pretzsch, H., Priesack, E., Schnyder, H., Treutter, D. (2005). The plant’s capacity in regulating resource demand. Plant Biol., 7(6), 560–580. https://doi.org/10.1055/s-2005-872981 DOI: https://doi.org/10.1055/s-2005-872981
  13. Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant., 15(3), 473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x DOI: https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  14. Nadaska, M., Erdelský, K., Čupka, P. (1990). Improving the Czechoslovakian Mentha piperita L. cv. Perpeta by in vitro micropropagation and stabilizing the component. Biologia (Bratislava), 45(11), 955–959.
  15. Ozdemir, F.A. (2017). Effects of 6-benzylaminopurine and α-naphthalene acetic acid on micropropagation from ten days old cotyledon nodes of Mentha spicata subsp. Spicat. Romanian Biotech. Lett., 22(3), 12554–12559.
  16. Pánczél, M., Eifert, J. (1960). Die Bestimung des Zuckerund Stärkegehaltes der Weinrebe mittels Anthronreagens. Mitt. Klosterneuburg, 10, 102–110.
  17. Pati, P.K., Rath, S.P., Sharma, M., Sood, A., Ahuja, P.S. (2006). In vitro propagation of rose-a review. Biotechnol. Adv., 24(1), 94–114. http://dx.doi.org/10.1016/j.biotechadv.2005.07.001 DOI: https://doi.org/10.1016/j.biotechadv.2005.07.001
  18. Phatak, S.V., Heble, M.R. (2002). Organogenesis and terpenoid synthesis in Mentha arvensis. Fitoterapia, 73(1), 32–39. https://doi.org/10.1016/S0367-326X(01)00347-1 DOI: https://doi.org/10.1016/S0367-326X(01)00347-1
  19. Raja H.D., Arockiasamy, D. (2009). In vitro propagation of Mentha viridis L. from nodal and shoot tip explants. Plant Tiss. Cult. Biotech., 18(1), 1–6. https://doi. org/10.3329/ptcb.v18i1.3243 DOI: https://doi.org/10.3329/ptcb.v18i1.3243
  20. Rajasekharan, P.E., Yadava, M.K., Shashidhara, S., Bhagyalakshmi, N. (2012). In vitro multiplication of Mentha piperata L. and estimation of secondary metabolites. IUP J. Genet. Evolution, 4(4), 39–43.
  21. Santoro, M., Nievas, F., Zygadlo, J., Giordano, W., Banchio, E. (2013). Effects of growth regulators on biomass and the production of secondary metabolites in peppermint (Mentha piperita) micropropagated in vitro. Am. J. Plant Sci., 4(5), 49–55. http://dx.doi.org/10.4236/ajps.2013.45A008 DOI: https://doi.org/10.4236/ajps.2013.45A008
  22. Sarwar, S., Zia, M., Rehman, R.U., Fatima, Z., Sial, R.A., Chaudhary, M.F. (2009). In vitro direct regeneration in mint from different explants on half strength MS medium. Afr. J. Biotech., 8(18), 4667–4671.
  23. Senthil, K., Kamraj, M. (2012). Direct shoot regeneration from internodal explants of Mentha viridis L. Int. J. Pharm. Sci. Res., 3(4), 1101–1103. http://dx.doi.org/10.13040/IJPSR.0975-8232.3(4).1101-03 DOI: https://doi.org/10.13040/IJPSR.0975-8232.3(4).1101-03
  24. Singleton, V.L., Rossi, J.A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic., 16(3), 144–158.
  25. Shelepova, O.V., Dilovarova, T.A., Gulevich, A.A., Olekhnovich, L.S., Shirokova, A.V., Ushakova, I.T., Zhuravleva, E.V., Konovalova, L.N., Baranova, E.N. (2021). Chemical components and biological activities of essential oils of Mentha × piperita L. from field-grown and field-acclimated after in vitro propagation plants. Agronomy, 11 (11), 2314. https://doi.org/10.3390/agronomy11112314 DOI: https://doi.org/10.3390/agronomy11112314
  26. Sunandakumari, C., Martin, K.P., Chithra, M., Sini, S., Madhusoodanan, P.V. (2004). Rapid axillary bud proliferation and ex vitro rooting of herbal spice, Mentha piperita L. Indian J. Biotech., 3, 108–112.
  27. Trevisan, S.C.C., Menezes, A.P.P., Barbalho, S.M., Guiguer, É.L. (2017). Properties of Mentha piperita: a brief review. World J. Pharm. Med. Res., 3(1), 309–313.
  28. Vaidya, B.N., Asanakunov, B., Shahin, L., Jernigan, H.L., Joshee, N., Dhekney, S.A. (2019). Improving micropropagation of Mentha × piperita L. using a liquid culture system. In Vitro Cell. Dev. Biol. Plant, 55, 71–80. https://doi.org/10.1007/s11627-018-09952-4 DOI: https://doi.org/10.1007/s11627-018-09952-4
  29. Varghese, T., Rema Shree, A.B., Nabeesa, E., Neelakandan, N., Nandakumar, S. (2003). In vitro propagation of Terminalia arjuna Roxb. multipurpose tree. Plant Cell Biotech. Mol. Biol., 4(1–2), 95–98.

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