Preliminary studies of polymorphism of selected microsatellite sequences in different colour types of raccoon dog (Nyctereutes Procyonoides)
Andrzej Jakubczak
University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 LublinGrażyna Jeżewska-Witkowska
University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 LublinBeata Horecka
University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 LublinMonika Żelazowska
University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 LublinEleonora Kopeć
University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 LublinZofia Rupeć
University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 LublinAbstract
Primary sequences designed for microsatellite loci in the domestic dog genome (FH2054, CPH3, FH2168, FH2164, FH2097), which were used in this study, also allowed for the
amplification of the corresponding loci in white and standard Raccoon dogs. In these groups the frequency of alleles, observed and expected heterozygosity, the polymorphic information content and the probability of parentage exclusion were analyzed. The studies allowed to define the expected degree of polymorphism of five microsatellite loci in the two color varieties of Raccoon dogs. All of the five selected microsatellite sequences may be used for checking the origin of Raccoon dogs and the construction of genetic maps within the canids. The obtained values of genetic similarity and genetic distance shows that there is a high genetic similarity between the groups of white and standard Raccoon dog genetic, and thus the small genetic distance. This suggests only small genetic differences between white and standard Raccoon dogs.
Keywords:
Raccoon dog, microsatellite, PCR-multiplexReferences
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University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 Lublin
University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 Lublin
University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 Lublin
University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 Lublin
University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 Lublin
University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 Lublin
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