Abstrakt
Białka z rodziny HSPB (małe białka szoku cieplnego) odgrywają funkcjonalną rolę w regulacji wewnątrzkomórkowych procesów (apoptoza, odpowiedź na stany zapalne, aktywność chaperonowa dotycząca kontroli fałdowania i agregacji białek) odpowiedzialnych za ochronę przed stresowymi czynnikami środowiskowymi. Mutacje genów kodujących te białka są przyczyną dysfunkcji komórek neuronowych, prowadzących do miopatii, neuropatii motorycznych i chorób neurodegeneracyjnych. Celem badań było cytogenetyczne mapowanie genów HSPB w genomie świni z zastosowaniem techniki FISH i sond uzyskanych z klonów BAC zawierających sekwencje genów HSPB1, HSPB2, CRYAB (alternatywna nazwa HSPB5), HSPB6, HSPB8, pochodzących z biblioteki genomowej CHORI-242 Porcine BAC Library. Przed hybrydyzacją in situ, przeprowadzoną na chromosomach metafazowych barwionych techniką prążków DAPI, potwierdzono obecność badanych genów w wyselekcjonowanym klonie metodą PCR z wykorzystaniem genowo specyficznych starterów.
W wyniku przeprowadzonych eksperymentów uzyskano sygnały FISH w regionach chromosomów
SSC3p15 (HspB1), SSC9p21 (HspB2 and CRYAB), SSC6q12 (HspB6) i HspB8 SSC14q21 (HspB8), co umożliwiło określenie fizycznej lokalizacji badanych genów HSPB na mapie genomowej świni domowej. Uzyskane wyniki mogą przyczynić się do wyjaśnienia roli genów HSPB w patomechanizmach miopatii i neuropatii u zwierząt hodowlanych.
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