Influence of amidrazones – 5-oxo-1,2,4-triazine derivative addition on the level of biochemical blood markers and chemical composition of turkey hens tissues

Abstrakt

The objective of this study was to determine the effect of 5-oxo-1,2,4- triazine derivative addition to water for turkey hens on the level of their biochemical blood markers and chemical
composition of their tissues. The study was conducted on 160 medium-heavy British United Turkey (BUT) 9 hens divided at random and equally into four groups, 40 turkey hens each (two replications × 20 birds). Since the 6^th till the 16^th weeks of life, the birds were kept in pens on straw litter. Group I served as a control (T0) and received drinking water without experimental additives. The turkey hens from experimental groups (T15, T30, T45) were administered three various doses of 5-oxo-1,2,4- triazine dissolved in 2 ml of ethanol with drinking water, i.e.: 15 μg kg b.w./day in group T15, 30 μg/kg b.w./day in group T30, and 45 μg/kg b.w./day in group T45. The additives examined were administered to the birds with drinking water for the period of 28 days. Four weeks of amidrazones derivative – 5-oxo-1,2,4- triazine – administration to the birds were followed by a two-week break in supplementation during which the turkey hens of all groups received only pure water for drinking. After this two-week-long period, the additives were again administered to the birds, in doses as at the beginning of the experiment. At the end of the 4^th, 6^th and 10^th weeks of observations, blood was sampled from the wing vein of the birds for biochemical analyses. The biochemical assays were carried out for levels of: AST, ALT, ALP, TP, GLU, CHOL, HDL, TG, and macroelements (K⁺, Na⁺, Ca⁺², Mg⁺²). On termination of rearing (16th week of life), the birds were slaughtered. The chemical composition of meat was determined in samples of breast, thigh, and shank muscles as well as in the liver. The application of various doses of 5-oxo-1,2,4- triazine derivative had no significant effect on the levels of the analyzed biochemical markers of turkey hens blood. An exception was the increased calcium content observed in the 11^th week of life. The administration of 5-oxo-1,2,4- triazine to turkey hens from groups T15, T30 and T45 contributed to their better mean body weight gains (by ca. 6.4%) as compared to the control birds. The addition of various doses of 5-oxo-1,2,4- triazine to drinking water was observed to decrease the content of crude fat in breast muscles and in liver, which increases the dietetic value of turkey hens meat.