Abstrakt
The present studies aimed at: 1) species identification of the isolates collected from the skin of the feet; 2) evaluation of the fungicidal properties of nanosilver towards isolated strains of the Candida genus.
The studies included 61 healthy individuals aged 20–60 who were assessed in relation to the occurrence of Candida yeast carriage. Fungal culture and identification were conducted according to the standard procedures. Evaluation of fungicidal operation of nanosilver was carried out against the isolated strains of Candida genus and the reference strain. During the study, the modi- fied dilution-neutralization method was applied according to the PN-EN 1275.
Swab samples collected from the skin of the lower extremities of 10 people (15.8%) was positive for the presence of fungi. The present studies showed that among the recovered yeasts, the pres- ence of C. albicans and C. guiliermondii was observed as well as single cases of C. parapsilosis, famata and C. lusitaniae. All the tested strains proved to be susceptible to nanosilver applica- tion, i.e. all the studied strains exhibited the reduction in viable cell counts within 60 min of expo- sure time.
Bibliografia
Anane S., Kallel K., Belhaj S., Chaker E., 2007. Candida dubliniensis: a novel emerging species.Ann. Biol. Clin. 65, 13–19.
Banach M., Kowalski Z., Pulit J., 2012. Zgłoszenie patentowe Nr P.399209, 17.05.2012.
Banach M., Kowalski Z., Wzorek Z., 2007. Nanosilver – production, antibacterial properties, application. Chemik 9, 435–438.
Banach M., Tymczyna L., Chmielowiec-Korzeniowska A., Pulit-Prociak J., 2016. Nanosilver biocidal properties and their application in disinfection of hatchers in poultry processing plants. Bioinorg. Chem. Appl. ID 5214783.
Chmielowiec-Korzeniowska A., Krzosek Ł., Tymczyna L., Pyrz M., Drabik A., 2013. Bactericidal, fungicidal and virucidal properties of nanosilver. Mode of action and potential application. A revive. Annales UMCS, sec. EE, Zootechnica 31, 1–11.
Czyż K., Dobrzański Z., Patkowska-Sokoła B., Opaliński S., 2013. Comparison of an effective- ness of aqueous and alcoholic silver nanoparticle suspensions in decreasing the ammonia emissions from poultry litter. Przem. Chem. 7, 1318–1322.
Falkiewicz-Dulik M., Macura A.B., 2006. Footwear hygiene in the foot mycosis prophylaxis. Med. Mycol. 13, 265–271.
Ioannidou D.J., Maraki S., Krasagakis S.K., Tosca A., Tselentis Y., 2006. The epidemiology of onychomycoses in Crete, Greece, between 1992 and 2001. J. Eur. Acad. Dermatol. Venereol. 20, 170–174.
Jagielski T., 2006. Protothecosis – etiology, clinical picture, therapy and laboratory diagnostics. Med. Mycol. 13, 307–313.
Khaled H., Hamad A.M., 2012. Changing epidemiology of classical and emerging human fungal infections: A review. Jordan J. Biol. Sci. 5, 215–230.
Kim K.J., Sung W.S., Moon S.K., Choi J.S., Kim J.G., Lee D.G., 2008. Antifungal effect of silver nanoparticles on dermatophytes. J. Microbiol. Biotechnol. 18, 1482–1484.
Li W.R., Xie X.B., Shi Q.S., Zeng H.Y., Ou-Yang Y.S., Chen Y.B., 2010. Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli. Appl. Microbiol. Biotechnol. 85, 1115–1122.
Luo D.Q., Yang W., Wu L.C., Liu J.H., Chen W.N., 2011. Interdigital ulcer: an unusual presenta- tion of Candida infection. Mycoses 54, 780–784.
Monteiro D.R., Gorup L.F., Takamiya A.S., Ruvollo-Filho A.C., de Camargo E.R., Bartosa D.B., 2009. The growing importance of materials that prevent microbial adhesion: antimicrobial ef- fect of medical devices containing silver. Int. J. Antimicrob. Agents 34, 103–110.
Nozari S., Haydari K.F., Ashrafi K, Ahmadi H., Ghasemi Z, Nami S., 2012. Comparison of anti- fungal effect of fluconazole alone and in combination with nanosilver particles against can- dida species isolated from chronic candidal vulvovaginitis. Razi. J. Med. Sci. 18, 8–14.
Pal S., Tak Y.K., Song J.M., 2007. Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the Gram-negative bacterium Escherichia coli. Appl. Environ. Microbiol. 73, 1712–1720.
PN-EN 1275. Chemical disinfectants and antiseptics – Quantitative suspension test for the evalua- tion of basic fungicidal or basic yeasticidal activity of chemical disinfectants and antiseptics – Test method and requirements (phase 1).
Ravindran A., Chandran P., Khan S.S., 2013. Biofunctionalized silver nanoparticles: Advances and prospects. Colloids Surf., B Biointerfaces 105, 342–352.
Raz-Pasteur A., Ullmann Y., Berdicevsky I., 2011. The pathogenesis of Candida infections in a human skin model: Scanning electron microscope observations. ISRN Dermatology, doi.org/10.5402/2011/150642.
Richardson M.D., 2005. Changing patterns and trends in systemic fungal infections. J. Antimi- crob. Chemother. 56, Suppl. S1, 5–11.
Richardson M.D., Warnock D.W., 2012. Fungal infection: diagnosis and management, 4th edn.Willey-Blackwell. Oxford.
Shamim S.S., Waseemuddin A., Iqbal A., Mohtasheemul H.A., Husan B., 2003. Superficial myco- sis in Sindh region. Pak. J. Pharm. 20, 27–35.
Sondi I., Salopek-Sondi B., 2004. Silver nanoparticles as antimicrobial agent: a case study onE. coli as a model for Gram-negative bacteria. J. Colloid Interface Sci. 275, 177–182.
Soumitra M.S., Saswati I., Prajna J., Biju J.S., Biplab S., Avinash S., 2012. An investigation on the antibacterial, cytotoxic, and antibiofilm efficacy of starch-stabilized silver nanoparticles. Nanomed-Nanotechnol. 8, 916–924.
Xu C., Gao C., Zhang H., Chen J., 2013. In vitro activity of nano-silver against pulmonary patho- genic fungi. Life Sci. J. 10, 3040–3043.
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