The aim of the experiment was agrochemical evaluation of the influence of liming and fertilization with ammonium or nitrate nitrogen on the content of available forms and mineral fractions of phosphorus in soil. The content of available phosphorus was determined in distilled water, acid sodium carbonate and according to Egner-Riehm method. The mineral fractions of phosphorus were determined according to Chang and Jackson method with modifications according to Askinazi, Ginzburg and Lebiedewa. The obtained results indicated that the greatest increase of available phosphorus was caused by fertilization with superphosphate at higher levels. The application of liming influenced the increase of available phosphorus in soil. The effect of fertilization with various nitrogen forms and nitrogen levels on the content of available phosphorus was not significant. Liming as well as fertilization with calcium nitrate caused the increase of easily soluble fraction in the soil. The content of aluminum phosphates was higher in treatments with lower pH values. The content of calcium phosphates was the highest in treatments limed and fertilized with calcium nitrate at a higher rate.