The purpose of the study was the analysis of the weed seed bank depending on different methods of field management. The effects of different methods of galega fallow was estimated biomass cutting and removing from field (D, F), without cut down and removing (C, E), compared was estimated with grassland fallow (B) and cultivation land (A) on the weed seed bank. Galega fallow as well as grassland fallow reduced the weed seed bank. Better results were achieved when galega biomass was cut down and removed from the field. The number of weed species on cultivation field was higher than on fallow land, Spergula arvensis, Galinsoga parviflora and Chenopodium album are the dominating species. On fallow the dominating Spergula arvensis, Chenopodium album are characterize by good adaptation and longevities. Despite a higher weed species structure on the cultivating field mainly 3 weed species dominated (approximately 90% of all weed seeds). On fallow (depending on management) from 5 to 9 species constistuted the same percentage of weed seed bank.