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Vol. 73 No. 1 (2018)

Articles

Using the next generation DNA sequencing (technology NGS) in gene banks and plant breeding. A review

DOI: https://doi.org/10.24326/asx.2018.1.1
Submitted: December 28, 2018
Published: 2018-04-09

Abstract

NGS technology is a universal diagnostic tool in molecular biology. It is used for sequencing genomes and transcripts, protein–DNA/RNA interactions, methylation tests, and metagenomic studies. The technique allows for the analysis of different DNA fragments represented by multiple copies during a single reaction, library preparation and obtaining a genomic gigabase from a single sequencing. By NGS technology, the number of research probes and the reliability of sequencing results are increased.

This is especially valuable when the variation between genotypes is small. The costs and period of the sequencing process are repeatedly lower than compared with the capillary sequencer. NGS technology is useful for learning new SNPs and other variants of diversity, like deletions or indels. A specific bioinformatic analysis of the sequencing results will allow to acquire knowledge about genetic diversity of plants within a single species, more precise mapping of quantitative traits loci, accurate taxonomic identification of objects and assigning them to specific species, which is very important in GenBank collections. The article presents the available sequencing technologies with their precise characteristics. The authors aim is to update information on the latest advances in sequencing technology and their use in plant biotechnology.

 

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