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ASPHC Baner

Vol. 20 No. 1 (2021)

Articles

Effects of static magnetic field on growth, some biochemical and antioxidant system in lemon balm (Melissa officinalis L.) seedlings

DOI: https://doi.org/10.24326/asphc.2021.1.7
Submitted: October 8, 2019
Published: 2021-02-26

Abstract

The effects of magnetic waves as natural environmental factors on the Earths are not well known on plant growth and development. The present study was carried out to evaluate the effects of static magnetic field (SMF) treatment (4 and 6 mT for 30 and 120 min per day) for eight days on the biomass production, proline contents and total soluble sugar, phenolic compounds, accumulation of H2O2 and MDA along with activity of antioxidant enzymes in lemon balm seedlings. Our results showed that SMF treatments, especially 6 mT and 120 min duration, increased the plant biomass, proline contents, phenolic compounds, H2O2 and MDA accumulation, and reduced the contents of total soluble sugars. The SMF application also increased the activity of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) enzymes compared to untreated seedlings. Our results suggest that SMF treatments induces the antioxidant defense system in the lemon balm seedlings and, by changing the plant metabolism, improves the early vigor of seedlings.

References

  1. Abdollahi, F., Amiri, H., Niknam, V., Ghanati, F., Mahdigholi, K. (2018). Effects of static magnetic fields on the antioxidant system of almond seeds. Russ. J. Plant Physiol., 66(2), 299–307.
  2. Asghar, T., Jamil, Y., Iqbal, M., Abbas, M. (2016). Laser light and magnetic field stimulation effect on biochemical, enzymes activities and chlorophyll contents in soybean seeds and seedlings during early growth stages. J. Photochem. Photobiol. B, Biol., 165, 283–290.
  3. Ashraf, M.Y., Afaf, R., Qureshi, M.S, Sarwar, G., Naqvi, M.H. (2002). Salinity induced changes in α-amylase and protease activities and associated metabolism in cotton varieties during germination and early seedling growth stages. Acta Physiol. Plant., 24, 37–44.
  4. Bailly, C., Benamar, A., Corbineau, F., Come, D. (1996). Changes in malondialdehyde content and in superoxide dismutase, catalase, and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated ageing. Physiol. Plant., 97, 104–110.
  5. Bates, L.S., Waldren, R.P., Teare, I.D. (1973). Rapid determination of free proline for water-stress studies. Plant Soil, 39, 205–207.
  6. Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72(1–2), 248–254.
  7. Brand-Williams, W., Cuvelier, M.E., Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food. Sci. Technol., 28, 25–30.
  8. Carlberg, I., Mannervik, B. (1985). Glutathione reductase. Meth. Enzymol., 113, 484–490.
  9. Chaplin, C. (2005). Magnetic and electric effects on water. Available: http://www.Lsbu ac.uk/water/magnetic [date of access: 28.05.2005].
  10. De Souza, A., Garcia, D., Sueiro, L., Gilbert, F., Licea, L., Porras, E. (2006). Pre-sowing magnetic treatment of tomato seeds increase the growth and yield of plants. Bioelectromagnetics, 27, 173–184.
  11. Ghanati, F., Abdolmaleki, P., Vaezzadeh, M., Rajabbeigi, E., Yazdani, M. (2007). Application of magnetic field and iron in order to change medicinal products of Ocimum basilicum. Environmentalist, 27(4), 429–434.
  12. Ghorbani, A., Razavi, S.M., Ghasemi Omran, V.O., Pirdashti, H. (2018a). Piriformospora indica inoculation alleviates the adverse effect of NaCl stress on growth, gas exchange and chlorophyll fluorescence in tomato (Solanum lycopersicum L.). Plant Biol., 20(4), 729–736.
  13. Ghorbani, A., Razavi, S.M., Ghasemi Omran, V.O., Pirdashti, H. (2018b). Piriformospora indica alleviates salinity by boosting redox poise and antioxidative potential of tomato. Russ. J. Plant Physiol., 65(6), 898–907.
  14. Giannopolitis, C.N., Ries, S.K. (1977). Superoxide dismutases: I. Occurrence in higher plants. Plant Physiol., 59, 309–314.
  15. Haghighat, N., Abdolmaleki, P., Ghanati, F., Behmanesh, M., Payez, A. (2014). Modification of catalase and MAPK in Vicia faba cultivated in soil with high natural radioactivity and treated with a static magnetic field. J. Plant Physiol., 171(5), 99–103.
  16. Heath, R.L., Packer, L. (1968). Photoperoxidation in isolated chloroplasts. Arch. Biochem. Biophys., 125, 189–198.
  17. Huang, R., Sukprakarn, S., Phavaphutanon, L., Juntakool, S., Chaikul, C. (2006). Changes in antioxidant enzyme activity, lipid peroxidation and seedling growth of cucumber seed induced by hydropriming and electric field treatments. Kasetsart J., 40, 825–834.
  18. Iqbal, M., Haq, Z. ul, Malik, A., Ayoub, C.M., Jamil, Y., Nisar, J. (2016). Pre-sowing seed magnetic field stimulation: A good option to enhance bitter gourd germination, seedling growth and yield characteristics. Biocatal. Agric. Biotechnol., 5, 30–37.
  19. Irigoyen, J.J., Einerich, D.W., Sanchez-Diaz, M. (1992). Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiol. Plant., 84, 55–60.
  20. Jouni, F.J., Abdolmalekia, P., Ghanati, F. (2012). Oxidative stress in broad bean (Vicia faba L.) induced by static magnetic field under natural radioactivity. Mutat. Res., 741(1–2), 116–21.
  21. Leung, A.Y., Foster, S. (1996). Encyclopedia of Common Natural Ingredients Used in Food, Drugs and Cosmetics, 2nd ed. New York, John Wiley & Sons, Inc.
  22. Maffei, M.E. (2014). Magnetic field effects on plant growth, development and evolution. Front. Plant Sci., 4(5), 445.
  23. Malinowska, I., Studziński, M., Malinowski, H., Gadzikowska, M. (2017). Retention and separation changes of ternary and quaternary alkaloids from Chelidonium majus L. by TLC under the influence of external magnetic field. Chromatographia, 80, 923–930.
  24. Nakano, Y., Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol., 22, 867–880.
  25. Sari, A.O., Ceylan, A. (2002). Yield characteristics and essential oil composition of lemon balm (Melissa officinalis L.) grown in the Aegean Region in Turkey. Turk. J. Agric. For., 26(4), 217–224.
  26. Schultze, W., Zaglein, A., Hose, S., Kubeczka, K.H., Czygan, F.C. (1992). Volatiles in flowers of balm (Melissa officinalis L.). In: Advances in Labiate Science, Harley, R.M., Reynolds, T. (eds.). The Royal Botanic Gardens, UK, 357–366.
  27. Selim, A.H., El-Nady, M.F. (2011). Physio-anatomical responses of drought stressed tomato plants to magnetic field. Acta Astronaut., 69, 387–396.
  28. Sen, A., Alikamanoglu, S. (2014). Effects of static magnetic field pretreatment with and without PEG 6000 or NaCl exposure on wheat biochemical parameters. Russ. J. Plant Physiol., 61, 646–655.
  29. Serdyukov, Y.A., Novitskii, Y.I. (2013). Impact of weak permanent magnetic field on antioxidant enzyme activities in radish seedlings. Russ. J. Plant Physiol., 60, 69–76.
  30. Shao, H.B., Chu, L.Y., Shao, M.A., Jaleel, C.A., Mi, H.M. (2008). Higher plant antioxidants and redox signaling under environmental stresses. C. R. Biol., 433, 331–341.
  31. Velikova, V., Yordanov, I., Edreva, A. (2000). Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Plant Sci., 151, 59–66.
  32. Verma, O., Joshi, N., Pandey, S.T., Srivastava, R.C. (2017). Comparative study of hydropriming to static magnetic field on seedling vigour and enzyme activity in wheat seed. Agric. Res., 6(3), 235–240.
  33. Zhishen, J., Mengcheng, T., Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem., 64(4), 555–559.

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