Abstract
In this study in vitro root culture protocol was elaborated in order to diversify screening methods and develop quick and reliable assay to test the level of chosen
Daphne genotype resistance to fungal pathogen Thielaviopsis basicola. Plantlets of Daphne caucasica, D. cneorum, D. jasminea, and D. pontica were propagated aseptically on medium composed of WPM mineral salts and MS vitamins, supplemented with 1.0 mg dm-3 2iP, 0.1 mg dm-3 NAA, 0.5 g dm-3 PVP, 0.5 g dm-3 MES, 0.65 g dm-3 calcium gluconate, and 20 g dm-3 sucrose. Root cultures were initiated from adventitious roots regenerated on micropropagated shoots. Proliferative root cultures of examined Daphne species were obtained both on solidified and liquid medium, supplemented with various doses of NAA. Cultures of detached roots proved to be a convenient system of testing to T. basicola resistance. Daphne roots cultured in vitro were distinctly contaminated under laboratory conditions. Susceptibility of cultured organs differed between species. This simple method is appropriate to be put into practice for quick selection of resistant/
tolerant genotypes to the soil-borne fungal pathogens affecting plants via root system.
References
Allison C.C., 1938. Physiologic specialization of Thielaviopsis basicola on tobacco. Phytopathology 28, 1.
Altman A., 1999. Plant biotechnology in the 21st century: the challenges ahead. Elect. J. Biotech. www.ejb.org/content/vol2/issue2/full/1.
Brunelli A., 2010. A new background in the protection of plants from biotic pathogens. J. Plant Pathol. 92, suppl. 4, 447.
Compton M.E., 1994. Statistical methods suitable for the analysis of plant tissue culture data. Plant Cell Tiss. Organ Cult. 37, 217–242.
D’Ovidio R., Janni M., Volpi C., Rochchi V., 2010. Cell wall reinforcement for wheat improvement against fungal pathogen. J. Plant Pathol. 92, suppl. 4, 449.
Grasser R., Kern H., Defago G., 1988a. Resistance of tobacco to Thielaviopsis basicola under tissue culture conditions and increased susceptibility after transformation with Agrobacterium tumefaciens T-DNA. J. Phytopathol. 123, 105–114.
Grasser R., Kern H., Defago G., 1988b. Scopolin, a biochemical marker for resistance to Thielaviopsis basicola in callus and crown-gall tissue cultures of tobacco. J. Phytopathol. 123, 115–123.
Halford N.G., 2012. Toward two decades of plant biotechnology: successes, failures, and prospects. Food Energy Sec. 1(1), 9–28.
Hanus-Fajerska E., Farfán L., Riseman A., 2008. Podatność wawrzynka główkowego na Thielaviopsis basicola w testach in vitro. Zesz. Probl. Post. Nauk Roln. 531, 57–62.
Hanus-Fajerska E., Wiszniewska A., Czaicki P., 2012. Effectiveness of Daphne L. (Thymelaeaceae) in vitro propagation, rooting of microshoots and acclimatization of plants. Acta Agrobot. 65(1), 21–28.
Hood M.E., Shew H.D., 1997. Initial cellular interactions between Thielaviopsis basicola and tobacco root hairs. Phytopathology 87, 228–235
Karban R., 2011. The ecology and evolution of induced resistance against herbivores. Funct. Ecol. 25, 339–347.
Lloyd G., McCown B., 1981. Commercially feasible micropropagation of mountain laurel (Kalmia latifolia) by use of shoot tip culture. Int. Plant Prop. Soc. Proc. 30, 421–427.
Miazzi M.M., Hajjeh H.R., Faretra F., 2010. An in vitro method to evaluate grapevine cultivars for Erysiphe necator susceptibility. In Vitro Cell Dev. Biol. Plant 46(4), 363–367.
Murashige T., Skoog F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497.
Nishimura M.T., Dangl J.L., 2010. Arabidopsis and the plant immune system. Plant J. 61, 1053–1066.
Noshad D., Punja Z.K., Riseman A., 2006. First report of Thielaviopsis basicola on Daphne cneorum. Can. J. Plant Pathol. 28, 310–312.
Noshad D., Punja Z.K., Riseman A., 2007. Evaluation of Daphne germplasm for resistance of Daphne Sudden Death Syndrome caused by the soil-borne pathogen Thielaviopsis basicola. HortSci. 42, 1639–1643.
Noshad D., 2007. Daphne Sudden Death Syndrome (DSDS). Pathogen identification, characterization and screening for disease resistance. Vancouver, Canada, University of British Columbia, PhD Thesis.
Orsini F., Cascone P., De Pascale S., Barbieri G., Corrado G., Rao R., Maggio A., 2010. Systemin-dependent salinity tolerance in tomato: evidence of specific convergence of abiotic and biotic stress responses. Physiol. Plant. 138, 10–21.
Punja Z.K., 2001. Genetic engineering of plants to enhance resistance to fungal pathogens, a review of progress and future prospects. Can. J. Plant Pathol. 23, 216–235.
Sowik I., Michalczuk L., Wójcik D., 2008. A method for in vitro testing strawberry susceptibility to Verticillum wilt. J. Fruit Ornam. Plant Res. 16, 111–121.
Švabova L., Lebeda A., 2005. In vitro selection for improved plant resistance to toxin-producing pathogens. J. Phytopathol. 153, 52–64.
van den Bulk R.W., 1991. Application of cell and tissue culture and in vitro selection for disease resistance breeding – a review. Euphytica 56, 269–285.
Tilman D., Cassman K.G., Matson P.A., Naylor R., Polasky S., 2002. Agricultural sustainability and intensive production practices. Nature 418, 671–677.
Wani S.H., Sanghera G.S., Singh N.B., 2010. Biotechnology and plant disease control – role of RNA interference. Am. J. Plant Sci. 1, 55–68.
Zang Y., Shi J.Y., Zang J.D., Guo X.Q., 2010. Identification of novel NPR1-like gene from Nicotiana glutinosa and its role in resistance to fungal, bacterial and viral pathogens. Plant Biol. 12, 23–34.
Downloads
Download data is not yet available.
