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Vol. 11 No. 6 (2012)

Articles

CHANGES OF DEHYDROASCORBIC ACID CONTENT IN RELATION TO TOTAL CONTENT OF VITAMIN C IN SELECTED FRUITS AND VEGETABLES

Submitted: December 31, 2020
Published: 2012-12-31

Abstract

Vitamin C performs many functions in the human body and is very important for its proper functioning. The main vitamin C sources in the human diet are fruits and
vegetables. Changes of total content of vitamin C (Tc), L-ascorbic acid (AA) and dehydro-L-ascorbic acid (DHA) in selected fruits and vegetables during storage at 20°C until spoilage were determined. A decrease of Tc and AA contents and an increase of DHA concentration were noted. Products of high acidity such as lemon and tomato were characterized by the highest retention of vitamin C. An increase of the DHA to Tc ratio (%) was observed. On the first day of storage the DHA/Tc ratio was higher than 10% in broccoli, cucumber and banana, while on the last day – it amounted over 40% in banana and cucumber, between 20 and 40% in parsley leaves and broccoli, and below 20% in tomato, cauliflower and lemon. In order to obtain correct results of vitamin C determination in fruits and vegetables it is necessary to take DHA content into consideration.

References

Albrecht J.A., Schafer H.W., Zottola E.A., 1990. Relationship of total sulfur to initial and retained ascorbic acid in selected cruciferous and noncruciferous vegetables. J. Food Sci., 55, 181–183.
Arya S.P., Meenakshi M., Preeti J., 1998. Photometric Methods for the Determination of Vitamin C. Anal. Sci., 14, 889–895.
Ball G.F.M., 2006. Vitamins In Foods: Analysis, Bioavailability, and Stability. Taylor & Francis Group, Boca Raton, 679.
Block G., 1992. Human data on vitamin C in cancer prevention. In Bray G.A. and Ryan D.H. (ed.) Vitamin and cancer prevention. Pennington Center Nutrition Series, LSU Press, Baton Rouge, LA. 3, 247–255.
Catani M.V., Saviani I., Rossi A., Melino G., Avigliano L., 2005. Biological role of vitamin C in keratinocytes. Nutr. Rev., 63(3), 81–90.
Chan A.C., 1993. Partners in defense, vitamin E and vitamin C. Can. J. Physiol. Pharmacol., 71(9), 725–731.
Davey M.W., Van Montagu M., Inzé D., Sanmartin M., Kanellis A., Smirnoff N., Benzie I.J.J., Strain J.J., Favell D., Fletcher J., 2000. Plant L-ascorbic acid: chemistry, function, metabolism, bioavailability and effects of processing. J. Sci. Food Agric., 80, 825–860.
Frenich A.G., Torres M.E., 2005. Determination of ascorbic acid and carotenoids in food commodities by liquid chromatography with mass spectrometry detection. J. Agric. Food. Chem. 53(19), 7371–7376.
Gökmen V., Kahraman N., Demir N., Acar J., 2000. Enzymatically validated liquid chromatographic method for the determination of ascorbic and dehydroascorbic acids in fruit and vegetables. J. Chromatogr., A 881, 309–316.
Hunt J.R., Mullen L.M., Lykken G.I., Gallagher S.K., Nielsen F.H., 1990. Ascorbic acid: effect on ongoing iron absorption and status in iron depleted young woman. Am. J. Clin. Nutr., 51(4), 649–655.
Koh E., Wimalasiri K.M.S., 2009. Content of ascorbic acid, quercetin, kaempferol and total phenolics in commercial broccoli. J. Food Compos. Anal., 22(7–8), 637–643.
Lee S.K., Kader A.A., 2000. Preharvest and postharvest factors influencing vitamin C content of horticultural crops. Postharvest Biol. Tec., 20, 207–220.
Lykkesfeldt J., 2000. Determination of Ascorbic Acid and Dehydroascorbic Acid in Biological Samples by High-Performance Liquid Chromatography Using Subtraction Methods: Reliable Reduction with Tris[2-carboxyethyl]phosphine Hydrochloride. Anal. Biochem., 282, 89–93.
Nagy S., 1980. Vitamin C contents of citrus fruit and their products: a review. J. Agric. Food Chem., 28, 8–18.
Nováková L., Solich P., Solichová D., 2008. HPLC methods for simultaneous determination of ascorbic and dehydroascorbic acids. Trends Anal. Chem., 27(10), 942–957.
Nyyssonen K., Salonen J.T., Parviainen M.T., 2000. Ascorbic acid in Modern Chromatographic Analysis of Vitamins. De Leenheer, A.P., Lambert, W.E., Van Bocxlaer, J.F., Marcel Dekker, New York, 287.
Rapisarda P., Pannuzzo P., 2008. Effect of cold storage on vitamin C, phenolics and antioxidant activity of five orange genotypes [Citrus sinensis (L.) Osbeck]. Postharvest Biol. Tec., 49, 348–354.
Sato Y., Uchiki T., Iwama M., Kishimoto Y., Takahashi R., Ishigami A., 2010. Determination of dehydroascorbic acid in mouse tissues and plasma by using tris(2-carboxyethyl)phosphine hydrochloride as reductant in metaphosphoric acid/ethylenediaminetetraacetic acid solution. Biol. Pharm. Bull., 33(3), 364–369.
Valente A., Albuquerque T.G., 2011. Ascorbic acid content in exotic fruits: a contribution to produce quality data for food composition databases. Food Res. Int., 44(7), 2237–2242.
Wechtersbach L., Cigić B., 2007. Reduction of dehydroascorbic acid at low pH. J. Biochem. Biophys. Methods, 70, 767–772.
Wills R.B.H., Wimalasiri P., Heather G., 1984. Dehydroascorbic acid levels in fresh fruit and vegetables in relation to total vitamin C activity. J. Agric. Food Chem., 32, 836–838.
Zhang D., Hamauzu Y., 2004. Phenolics, ascorbic acid, carotenoids and antioxidant activity of broccoli and their changes during conventional and microwave cooking. Food Chem., 88(4), 503–509.
Zhang H.Y., 2003. Phenolic compounds, ascorbic acid, carotenoids and antioxidant properties of green, red and yellow bell peppers. J. Food Agric. Environ., 2, 22–27.

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