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ASPHC Baner

Vol. 25 No. 1 (2026)

Articles

research paper

In vitro assessment of drought tolerance in summer squash (Cucurbita pepo L.) cultivars

DOI: https://doi.org/10.24326/asphc.2026.5590
Submitted: 28 August 2025
Published: 27.02.2026

Abstract

In this study, the growth performance of three different summer squash cultivars was evaluated under in vitro conditions using Murashige and Skoog (MS) nutrient media supplemented with varying concentrations of polyethylene glycol (PEG) at 0%, 2%, 4%, and 6%. In the study, parameters including germination rate (%), stem fresh and dry weight (g), root fresh and dry weight (g), shoot and root length (mm) were investigated. In addition, antioxidant capacity and total phenolic content in plant shoots were determined using the DPPH method. According to the findings, the highest stem fresh weight was recorded as 0.72 g in cultivar Pelin grown in control medium (without PEG). In terms of root fresh weight, the highest mean value among the media was also observed in control with 0.43 g. The longest shoot length as 48.91 mm was also determined in Pelin. Increasing concentrations of PEG were found to have a negative impact on all growth parameters.

References

  1. Adem, E.İ., Kaplan Evlice, A., Kara, E. et al. (2025). Impact of drought stress on growth, phenolic content, and antioxidant activity in tomato (Solanum lycopersicum L.) cultivars under ın vitro conditions. Biol. Bull., 52, 330. https://doi.org/10.1134/S1062359025610936
  2. Adnan, M., Gul, S., Batool, S. et al. (2017). A review on the ethnobotany, phytochemistry, pharmacology and nutritional composition of Cucurbita pepo L. J. Phytopharm., 6(2), 133–139.
  3. Ahmad, M.A., Javed, R., Adeel, M. et al. (2020). PEG 6000-stimulated drought stress improves the attributes of in vitro growth, steviol glycosides production, and antioxidant activities in Stevia rebaudiana Bertoni. Plants, 9(11), 1552.
  4. Ainsworth, E.A., Gillespie, K.M. (2007). Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocâlteu reagent. Nat. Prot., 2, 875–877. https://doi.org/10.1038/nprot.2007.102
  5. Akram, S., Ghaffar, M., Wadood, A. et al. (2022). Development of better wheat plants for climate change conditions. In: Wheat. Recent advances. IntechOpen, London.
  6. Akte, J., Yasmin, S., Bhuiyan, M.J.H. et al. (2016). In vitro screening of rice genotypes using polyethylene glycol under drought stress. Progr. Agric., 27(2), 128–135.
  7. Ali, S., Mir, R.A., Haque, M.A. et al. (2025). Exploring physiological and molecular dynamics of drought stress responses in plants: challenges and future directions. Front. Plant Sci., 16, 1565635. https://doi.org/10.3389/fpls.2025.1565635
  8. Ayaz, M., Ahmad, R., Shahzad, M. et al. (2015). Drought stress stunt tomato plant growth and up-regulate expression of SlAREB, SlNCED3, and SlERF024 genes. Sci. Hortic., 195, 48–55. https://doi.org/10.1016/j.scienta.2015.08.025
  9. Bouchyoua, A., Kouighat, M., Hafid, A. et al. (2024). Evaluation of rapeseed (Brassica napus L.) genotypes for tolerance to PEG (polyethylene glycol) induced drought at germination and early seedling growth. J. Agric. Food Res., 15, 100928. https://doi.org/10.1016/j.jafr.2023.100928
  10. Brdar-Jokanović, M.D., Zdravković, J. (2015). Germination of tomatoes under PEG-induced drought stress. Ratar. Povrt., 52(3), 108–113. https://doi.org/10.5937/ratpov52-8324
  11. Conti, S., Villari, G., Amico, E., et al. (2015). Effects of production system and transplanting time on yield, quality and antioxidant content of organic winter squash (Cucurbita moschata Duch.). Sci. Hortic., 183, 136–143. https://doi.org/10.1016/j.scienta.2014.12.003
  12. Devincentis, A.J. (2020). Scales of sustainable agricultural water management. Dissertation. University of California, Davis, CA, USA.
  13. Dietz, K.J., Zörb, C., Geilfus, C.M. (2021). Drought and crop yield. Plant Biol., 23(6), 881–893. https://doi.org/10.1111/plb.13304
  14. Dolgun, C., Çifci, E.A. (2018). Farklı kuraklık stresi seviyelerinin makarnalık buğday çeşitlerinde çimlenme ve erken fide gelişimi üzerine etkisi [Effects of different drought stress levels on the germination and early seedling growth in durum wheat]. Bursa Uludağ Üniversitesi Ziraat Fakültesi Dergisi, 32(2), 99–109.
  15. Caili, F., Huan, S., Quanhong, L.A. (2006). Review on pharmacological activities and utilization technologies of pumpkin. Plant Foods Human Nutr., 61, 70–77. https://doi.org/10.1007/s11130-006-0016-6
  16. Garcia-Caparros, P., Ciriello, M., Rouphael, Y. et al. (2025). The role of organic extracts and ınorganic compounds as alleviators of drought stress in plants. Horticulturae, 11(1), 91. https://doi.org/10.3390/horticulturae11010091
  17. Jezdinský, A., Petříková, K., Slezák, K. et al. (2012). Effect of drought stress and mycorrhizal inoculation on the growth, photosynthetic activity and water use efficiency of leek (Allium porrum L.Gigante Suizo). Acta Univ. Agric. Silvic. Mendel. Brun., 40(8), 101–108.
  18. Kara, E., Taşkın, H., Baktemur, G. (2024a). Pırasanın (Allium ampeloprasum var. porrum L.) İn Vitro Koşullarda Farklı PEG Dozlarına Tolerans Seviyesinin Belirlenmesi [Determination of tolerance level of leek (Allium ampeloprasum var. porrum L.) to different PEG doses ın vitro conditions]. III. International Korkut Ata Scientific Research Conference, 22–24 November 2024, Osmaniye, Türkiye, 89–97.
  19. Kara, E., Baktemur. G., Taşkın, H. (2024b). Beyaz Baş Lahana (Brassica oleracea var. capitata L.) Bitkisinin In Vitro Koşullarda Kuraklık Stresinin Belirlenmesi [Determination of drought stress of white head cabbage (Brassica oleracea var. capitata. L.) ın vitro conditions]. Tokyo 9th International Innovative Studies & Contemporary Scientific Research Congress, 10–11 October 2024, Tokyo, Japan, 452–459.
  20. Kara, E., Baktemur. G., Taşkın, H. (2024c). Farklı Kuraklık Konsantrasyonlarının Soğan (Allium cepa L.) Üzerine Etkilerinin In Vitro Koşullarda Belirlenmesi [Determination of the effects of different drought concentrations on onion (Allium cepa L.) ın vitro conditions]. III International Korkut Ata Scientific Research Conference, 22–24 November 2024, Osmaniye, Türkiye, 81–88.
  21. Kaya, G. (2025). Changes in morpho-physiological characteristics of melon (Cucumis melo L.) seedlings under different levels of drought stress. Hort. Stud., 42(2), 51–56. https://doi.org/10.16882/hortis.1663801
  22. Khan, Z., Jan, R., Asif, S. et al. (2024). Exogenous melatonin induces salt and drought stress tolerance in rice by promoting plant growth and defense system. Sci. Rep., 14, 1214. https://doi.org/10.1038/s41598-024-51369-0
  23. Koua, A.P., Oyiga, B.C., Baig, M.M. et al. (2021). Breeding driven enrichment of genetic variation for key yield components and grain starch content under drought stress in winter wheat. Front. Plant Sci., 12, 684205. https://doi.org/10.3389/fpls.2021.684205
  24. Kumar, P.A., Reddy, N.N., Lakshmi, N.J. (2017). PEG induced screening for drought tolerance in tomato genotypes. Int. J. Curr. Microbiol. Appl. Sci., 6(7), 168–181. https://doi.org/10.20546/ijcmas.2017.607.020
  25. Perez Gutierrez, R.M. (2016). Review of Cucurbita pepo (pumpkin) its phytochemistry and pharmacology. Med. Chem., 6(1), 12–21.
  26. Masuda, T., Yonemori, S., Oyama, Y. et al. (1999). Evaluation of the antioxidant activity of environmental plants. Activity of the leaf extracts from seashore plants. J. Agric. Food Chem., 47(4), 1749–1754.
  27. Mehmandar, M.N., Rasouli, F., Giglou, M.T. et al. (2023). Polyethylene glycol and sorbitol-mediated in vitro screening for drought stress as an efficient and rapid tool to reach the tolerant Cucumis melo L. Genotypes. Plants, 12(4), 870. https://doi.org/10.3390/plants12040870
  28. Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant., 15(3), 473–497.
  29. Passioura, J.B., Angus, J.F. (2010). Improving productivity crops water-limited environments. Adv. Agron., 106, 37–75. https://doi.org/10.1016/S0065-2113(10)06002-5
  30. Pérez-Jiménez, M., Pérez-Tornero, O. (2020). In vitro plant evaluation trial. Reliability test of salinity assays in citrus plants. Plants, 9(10), 1352. https://doi.org/10.3390/plants9101352
  31. Popović, B.M., Štajner, D., Ždero-Pavlović, R. et al. (2016). Water stress induces changes in polyphenol profile and antioxidant capacity in poplar plants (Populus spp.). Plant Physiol. Biochem., 105, 242–250. https://doi.org/10.1016/j.plaphy.2016.04.036
  32. Ratnam, N., Vandana, Naijibullah, M., Ibrahim, M. (2017). A review on Cucurbita pepo. Int. J. Pharmacog. Phytochem. Res., 9(9), 1190–1194. https://doi.org/10.25258/phyto.v9i09.10305
  33. Rehman, A., Khalid, M., Weng, J. et al. (2024). Exploring drought tolerance in melon germplasm through physiochemical and photosynthetic traits. Plant Growth Reg., 102(3), 603–618. https://doi.org/10.1007/s10725-023-01080-3
  34. Sahu, M., Maurya, S., Jha, Z. (2023). In vitro selection for drought and salt stress tolerance in rice: an overview. Plant Physiol. Rep., 28(1), 8–33. https://doi.org/10.1007/s40502-023-00713-z
  35. Seleiman, M.F., Al-Suhaibani, N., Ali, N. et al. (2021). Drought stress impacts on plants and different approaches to alleviate its adverse effects. Plants, 10(2), 259. https://doi.org/10.3390/plants10020259
  36. Stringer, L.C., Mirzabaev, A., Benjaminsen, T.A. et al. (2021). Climate change impacts on water security in global drylands. One Earth, 4(6), 851–864. https://doi.org/10.1016/j.oneear.2021.05.010
  37. Tajaragh, R.P., Rasouli, F., Giglou, M.T. et al. (2022). Morphological and physiological responses of in vitro-grown Cucurbita sp. landraces seedlings under osmotic stress by mannitol and PEG. Horticulturae, 8(12), 1117. https://doi.org/10.3390/horticulturae8121117
  38. Torun, A., Sarı, N. (2025). Drought tolerance levels of turkish melon genetic resources using morphological and physiological methods. Turk. J. Agric.-Food Sci. Technol., 13(4), 831–841. https://doi.org/10.24925/turjaf.v13i4.831-841.7640
  39. Tran, T.T., Bui, V.T., Tran, H.T. (2020). Effect of drought stress and thermal pre-treatment on the ın vitro shoot development of Solanum lycopersicum L. Chem. Eng. Trans., 78, 229–234. https://doi.org/10.3303/CET2078039
  40. Turan, F., Samur, S. (2024). Kolza (Brassica napus L.) Tohumuna Borik Asit ve Gibberellik Asit Ön Uygulamalarının Kuraklık Stresine Karşı Etkisinin İncelenmesi [Investigation of the effect of boric acid and gibberellic acid priming on rapeseed (Brassica napus L.) seeds against drought stress]. ISPEC J. Agric. Sci., 8(3), 756–765. https://doi.org/10.5281/zenodo.12788918
  41. Ulas, F., Kılıç, F.N., Ulas, A. (2025). Alleviate the ınfluence of drought stress by using grafting technology in vegetable crops: a review. J. Crop Health, 77(2), 51. https://doi.org/10.1007/s10343-025-01120-0
  42. Vives-Peris, V., Gómez-Cadenas, A., Pérez-Clemente, R.M. (2017). Citrus plants exude proline and phytohormones under abiotic stress conditions. Plant Cell Rep., 36(12), 1971–1984. https://doi.org/10.1007/s00299-017-2214-0
  43. Wickramasinghe, I.M., Seran, T.H. (2019). Assessing in vitro germination and seedling growth of tomato (Solanum lycopersicum L.) cv KC-1 in response to polyethylene glycol-induced water stress. Sri Lanka J. Food Agric., 5(2), 7–16. https://doi.org/10.4038/sljfa.v5i2.72
  44. Yıldırım, E., Kılıçaslan, S.C., Ekinci, M. et al. (2020). Kuraklık stresinin fasulyede bitki gelişimi, bazı fizyolojik ve biyokimyasal özellikler üzerine etkisi [The effect of drought stress on plant growth, some physiological and biochemical properties of bean]. Erciyes Üniversitesi Fen Bilimleri Enstitüsü Fen Bilimleri Dergisi [Erciyes Univ. J. Instit. Sci. Technol.], 36(2), 264–273.
  45. Zhang, C., Shi, S., Wang, B. et al. (2018). Physiological and biochemical changes in different drought-tolerant alfalfa (Medicago sativa L.) varieties under PEG-induced drought stress, Acta Physiol. Plant., 40, 25. https://doi.org/10.1007/s11738-017-2597-0
  46. Zayova, E., Philipov, P., Nedev, T. et al. (2017). Response of in vitro cultivated eggplant (Solanum melongena L.) to salt and drought stress. AgroLife Sci. J., 6(1), https://agrolifejournal.usamv.ro/index.php/agrolife/article/view/686.

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