Chemical profile of Nepeta cataria L. var. citriodora (Becker) essential oil and in vitro evaluation of biological activities
Milica Acimovic
Institute of Field and Vegetable Crops Novi Sadhttps://orcid.org/0000-0002-5346-1412
Vanja Seregelj
Faculty of Technology, University of Novi Sad, Serbiahttps://orcid.org/0000-0003-0302-0851
Katarina Simić
Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Serbiahttps://orcid.org/0000-0001-5060-8936
Ana Varga
Institute of Food Technology, University of Novi Sad, SerbiaLato Pezo
Institute of General and Physical Chemistry, University of Belgrade, Serbiahttps://orcid.org/0000-0002-0704-3084
Jelena Vulić
Faculty of Technology, University of Novi Sad, Serbiahttps://orcid.org/0000-0001-9349-7367
Ivana Cabarkapa
Institute of Food Technology, University of Novi Sad, Serbiahttps://orcid.org/0000-0003-2215-4281
Abstract
Essential oil (EO) obtained by hydrodistillation in a Clevenger-type apparatus from aerial parts of Nepeta cataria L. var. citriodora (Becker), cultivated in Serbia was subjected to gas chromatography-mass spectroscopy (GC-MS) to determine the composition. Furthermore, N. cataria var. citriodora essential oil was
tested to determine its antimicrobial, antioxidant, antihyperglycemic and anti-inflammatory activities in vitro.
The antimicrobial activity was tested by broth microdilution method against 16 bacterial strains from American Type Culture Collection (ATCC). Four common tests for measuring in vitro antioxidant activity were used: 2, 2-diphenyl-1-picrylhydrazyl assay (DPPH), reducing power (RP), 2,2-azino-bis-3-ethylbenzothiazoline-
6-sulfonic acid (ABTS) and β-carotene bleaching assay (BCB). Antihyperglycemic activity was examined by using α-glucosidase inhibitory potential (AHgA), while anti-inflammatory activity (AIA) was determined by protein denaturation bioassay, using egg albumin. In total, 36 compounds were isolated and detected by GC-MS technique in N. cataria var. citriodora EO. The EO is mainly comprised of oxygenated monoterpenes (93.1%), and the main compounds were two monoterpenoid alcohols, nerol (38.5%) and geraniol (24.9%), followed by two aliphatic aldehyde, geranial (14.6%) and neral (11.0%). Antimicrobial activity of this EO shows growth inhibition of all tested bacteria strains, and exhibited good antioxidant, antihyperglycemic and anti-inflammatory activities. The EO obtained from N. cataria var. citriodora grown in Serbia shows valuable biological activity, indicating its potential for use as a supplement in everyday diet and as a natural preservative in food industry.
Keywords:
lemon catnip, antimicrobial activity, antioxidant activity, antihyperglycemic activity, anti-inflammatory activityReferences
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Institute of Field and Vegetable Crops Novi Sad https://orcid.org/0000-0002-5346-1412
Faculty of Technology, University of Novi Sad, Serbia https://orcid.org/0000-0003-0302-0851
Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Serbia https://orcid.org/0000-0001-5060-8936
Institute of Food Technology, University of Novi Sad, Serbia
Institute of General and Physical Chemistry, University of Belgrade, Serbia https://orcid.org/0000-0002-0704-3084
Faculty of Technology, University of Novi Sad, Serbia https://orcid.org/0000-0001-9349-7367
Institute of Food Technology, University of Novi Sad, Serbia https://orcid.org/0000-0003-2215-4281
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