In vitro CULTURE OF BIG-SAGE (Lantana camara L.) PLANT
Majid Abdulhameed Ibrahim
College of Agriculture, University of Basrah, Basrah, IraqManal Zebari Sabty
College of Agriculture, University of Basrah, Basrah, IraqShaimaa Hussein Mussa
College of Agriculture, University of Basrah, Basrah, IraqAbstract
The study was conducted to mass micropropagation of big sage (Lantana camara L.) plant by shoot multiplication technique. The treatments 2.22 and 2.66 µmol·L–1 BA gave the highest significant increase in the percentage of response to shoot multiplication and number of shoots per explant compared to the other treatments as reached 96.70% and 100.00% and 4.33 and 6.00 shoots, respectively. The results showed that these two treatments did not differ significantly between them. While the 1.33 µmol·L–1 BA gave the lowest values in the percentage of response to shoot multiplication and number of shoots per explant were 80.00% and 2.00 shoots per explant, respectively. The MS medium supplemented with 4.30 or 5.37 µmol·L–1 NAA gave a high response to root formation, number of roots per shoot and root length. While the MS medium supplemented with 6.44 or 7.52 µmol·L–1 NAA gave low values in these characteristics. The MS medium with 2.22 or 2.66 µmol·L–1 concentration of BA or 7.52 µmol·L–1 concentration of NAA recorded the highest significant increase in the percentage of response to callus formation. While the MS medium supplemented with 1.33 µmol·L–1 BA or 4.30 µmol·L–1 NAA gave less response to the callus formation.
Keywords:
benzyl adenine, callus induction, explant, micropropagation rooting, shoot multiplicationReferences
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College of Agriculture, University of Basrah, Basrah, Iraq
College of Agriculture, University of Basrah, Basrah, Iraq
College of Agriculture, University of Basrah, Basrah, Iraq
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