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Vol. 32 No. 4 (2014)

Articles

Comparative hybridizations in situ for identification heterosomes in aoudad (Ammotragus lervia)

Submitted: August 2, 2019
Published: 2014-12-17

Abstract

Genetic conservation on the level of genes from syntenic groups enables the use of molecular probes obtained from one species of animals to detect homologous DNA segments in different species. The aim of this study was to identify heterosomes in aoudad (Ammotragus lervia) by comparative hybridizations in situ technique (Zoo-FISH) with commercial bovine heterosomes painting probes. The results obtained showed distinct yellow-green signals in big acrocentric chromosomes X and strong red fluorescence signals in small metacentric chromosomes Y in all aoudad metaphase plates. The experiments confirmed the high degree of genetic conservation of heterosome synteny groups in the species (cattle and aoudad) belonging to Bovidae family, which makes it possible to use bovine, heterologous sex chromosomes painting probes in interspecies comparative, phylogenetic and evolutionary studies.

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