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Vol. 29 No. 1 (2011)

Articles

Search for SNP mutations of chosen genes of the hypothalamic-pituitary axis in laying hens

Submitted: March 18, 2021
Published: 2011-03-31

Abstract

The analyses performed aimed at the detection of possible point mutations in genes from the multi-level neuro-hormonal system, whose products are expressed in various organs of the bird reproductive system. In future, they could be a basis in the search for relations between polymorphism and the level of hatchability traits. The study involved two lines: Rhode Island White (200 individuals) and Rhode Island Red (100 individuals) laying hens. Molecular analyses consisted in preliminary verification of the SNP mutation in selected gene fragments: Insulin-like growth factor 1 (IGF1, exon 3, GeneID: 418090, amplicon length 479 bp) and Progesterone receptor (PGR, intron 6, GeneID: 396198, amplicon length 362 bp). The PCR-RFLP reaction was the second step of the investigations. A point mutation in the 38965 gene nucleotide within exon 3 was detected as a result of the IGFI gene sequencing reaction. The sense mutation involved an adenineto-guanine nucleotide change. In both hen lines, allele A and a mutated allele B with a 50% frequency were identified using the PCR-RFLP method for further analyses. The PGR sequencing reaction revealed a mutation in nucleotide position 34216 in intron 6 of the gene. Here, transition of the adenine to guanine nucleotide occurred. Allele A was identified, after 155 bp and 207 bp fragments had been obtained in the restriction analysis, as well as a mutated 362 bp allele B. Heterozygosity in the study locus was 0.38 for the RIW line and 0.23 for RIR.

References

Amills M., Jiménez N., Villalba D., Tor M., Molina E., Cubiló D., Marcos C., Francesch A., Sánchez A., Estany J., 2003. Identification of three single nucleotide polymorphisms in the chicken insulin-like growth factor 1 and 2 genes and their associations with growth and feeding traits. Poult. Sci. 82, 1485–1493.
Bian L.H., Wang S.Z., Wang Q.G., Zhang S., Wang Y.X., Li H., 2008. Variation at the insulin-like growth factor 1 gene and its association with body weight traits in the chicken. J. Anim. Breed. Genet. 125, 265–270.
Camacho-Arroyo I., González-Arenas A., González-Morán G., 2007. Ontogenic variations in the content and distribution of progesterone receptor isoforms in the reproductive tract and brain of chicks. Comp. Biochem. Physiol., Part A 146, 644–652.
Duclos M.J., 2005. Insulin-like growth factor-I (IGF-1) mRNA levels and chicken muscle growth. J. Physiol. Pharmacol. 56, 3, 25–35.
Gahr M., 2001. Distribution of sex steroid hormone receptors in the avian brain: functional implications for neural sex differences and sexual behaviors. Micros. Res. Tech. 55, 1–11. http://frodo.wi.mit.edu/primer3/
Johnson P.A., Johnson A.L., Van Tienhoven A., 1985. Evidence for a positive feedback interaction between progesterone and luteinizing hormone in the induction of ovulation in the hen, Gallus domesticus. Gen. Comp. Endocrinol. 58, 478–485.
Kim M., Seo D.S., Ko Y., 2004. Relationship between egg productivity and insulin – like growth factor I genotypes in Korean Native Ogol Chickens. Poult. Sci. 83, 1203–1208.
Klein S., Morrice D.R., Sang H., Crittenden L.B., Burt D.W., 1996. Genetic and physical mapping of the chicken IGF1 gene to chromosome 1 and conservation of synteny with other vertebrate genomes. J. Hered. 87, 10–14.
Le Roith D., Bandy C., Yakar S., Liu J., Butler A., 2001. The somatomedin hypothesis. Endoc. Rev. 22, 1, 53–74.
Nagaraja S.C., Aggrey S.E., Yao J., Zadworny D., Fairfull R.W., Kuhlein U., 2000. Trait associations of a genetic marker near the IGF-1 gene in egg-laying chickens. J. Hered., 91, 2, 150–156.
Ott J., 1985. Analysis of human genetic linkage. Johns Hopkins University Press, Baltimore and London.
Rozempolska-Rucińska I., 2010. Reliability of breeding value estimation of laying hens for hatching characteristics. Ann. Anim. Sci. 10, 1, 49–55.
Rozempolska-Rucińska I., Zięba G., Łukaszewicz M., 2009. Hatchability traits as selection criteria in breeding of laying hens. Archiv für Geflügelkunde 73, 4, 263–267.
Rozempolska-Rucińska I., Zięba G., Łukaszewicz M., Ciechońska M., 2010. Hatchability as modelled with or without bird’s permanent environment effect due to hatch in laying hens. Archiv für Geflügelkunde 1, 74, 58–61.
Tang S., Sun D., Ou J., Zhang Y., Xu G., Zhang Y., 2010. Evaluation of the IGFs (IGF1 and IGF2) genes as candidates for growth, body measurement, carcass, and reproduction traits in Beijing You and Silkie chickens. Anim. Biotech. 21, 2,104–13.
Toye A. A., Bumstead N., Moran C., 1997. A pentanucleotide repeat polymorphism maps progesterone receptor (PGR) to chicken chromosome 1. Anim. Genet. 28, 4, 317.
Twito T., Weigend S., Blum S., Granevitze Z., Feldman M.W., Perl-Treves R., Lavi U., Hillel J., 2007. Biodiversity of 20 chicken breeds assessed by SNPs located in gene regions. Cytogen. Genome Res. 117, 319–326.
Zhou H., Mitchell A.D., McMurtry J.P., Ashwell C.M.,Lamont S.J., 2005. Insulin- Like growth factor gene polymorphism associated with growth, body composition, skeleton integrity, add metabolic traits in chickens. Poult. Sci. 84, 212–219.

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