Abstract
The aim of the study was identification of molecular markers useful in differentiating the species of selected yeast-like fungi on the skin and mucous membranes of horses and in identification of Candida rugosa strains. The study material was smears from horse skin and mucous membranes. The isolates were assayed with the use of API 20C AUX (bioMérieux) biochemical tests. The examinations involved C. lusitaniae, C. parapsilosis and C. rugosa. RAPD-PCR analyses included amplification with the use of 20 arbitrary primers (OPG01-OPG20). Genetic distances between the Candida rugosa strains were estimated by the Popgen32 programme. The RAPD-PCR analysis yielded amplification products of all the markers used in the study. The number of speciesspecific DNA fragments ranged from 0 to 6, depending on the primer. At least two specific DNA fragments were found for each of the studied species in the case of primers OPG04, OPG05, OPG06, OPG09, OPG11 and OPG14, which may indicate the possibility of their use for species identification. The present work is the first attempt to describe genetic differences between Candida rugosa strains sampled from healthy Hucul horses. Four genotypes within the Candida rugosa species, whose frequency ranged from 0.06 to 0.62, were obtained upon combination of DNA profiles of three primers (OPG04, OPG05 and OPG11). The genetic distances identified indicated considerable diversity of the studied strains. The genetic diversity may corroborate different environmental epidemiologic sources of the strains and may be related to their non-pathogenic nature.
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