Abstract
This article presents the results of a study on 10 microsatellite DNA loci polymorphism in Charolais cattle (n = 101). Seventy-seven allelic variants were identified and the largest number of alleles was observed for TGLA53 locus (12 alleles). All microsatellite DNA markers, except ETH10, showed a high degree of polymorphism. A significantly high B allele frequency (0.269) of kappa-casein gene was detected and a large number of animals (0.508) have the heterozygous AB genotype variant. The frequency of V allele (0.511) of the growth hormone gene was non-significantly higher than that of L allele (0.489). Animals with heterozygous LV genotype prevailed (0.537); frequencies of homozygous LL and VV genotypes were 0.221 and 0.242, respectively.
References
Biase F.H., Garnero A.D., Bezerra L.A., Rosa A.J., Lôbo R.B., Martelli L., 2005. Analysis of restriction fragment length polymorphism in the kappa-casein gene related to weight expected progeny difference in Nellore cattle. Genet. Mol. Biol. 28(1), 84–87.
Contreras V.P., Jaramillo D.L., Bracamonte G.M., Martinez Gonzalez J.C., Sifuentes Rincon A.M., 2011. Convenient genotyping of nine bovine K-casein variants. Electron. J. Biotechnol. 14(4).
Curi R.A., de Oliveira H.N., Silveira A.C., Lopes C.R., 2005. Association between IGF-I, IGF-IR and GHRH gene polymorphisms and growth and carcass traits in beef cattle. Livest. Prod. Sci. 94(3), 159–167.
Gibbs R.A., Taylor J.F., Van Tassell C.P., 2009. Genome-wide survey of SNP variation uncovers the genetic structure of cattle breeds. Science 324(5926), 528–532. Doi: 10.1126/science.1167936.
Jann O.C., Ibeagha-Awemu E.M., Özbeyaz C., Zaragoza P., Williams J.L., Ajmone-Marsan P., Lenstra J.A., Moazami-Goudarzi K., Erhardt G., 2004. Geographic distribution of haplotype diversity at the bovine casein locus. Genet. Sel. Evol. 36, 243–257.
Kawasaki E.S., 1990. Sample preparation from blood, cell and other fluids. In: PCR Protocols. A guide to methods and applications. Acadamic Press, New York, 146–152.
Kozubska-Sobocińska A., Radko A., Rejduch B., Słota E., 2008. Genetic conservation of Y chromosome basing on microsatellite sequences in some species of Bovidae. Ann. Anim. Sci. 8(3), 249–254.
Kozubska-Sobocińska A., Ząbek T., Słota E., Kaczor U., 2007. Comparison of GTG-banded karyotypes and microsatellite sequences in some species of the Bovidae and Cervidae families. J. Anim. Feed Sci. 16, 567–578.
Lara M., Gama L.T., Bufarah G., Sereno J.R.B., Celegato E.M.L., de Abreu U.P., 2002. Genetic polymorphisms at the K-casein locus in Pantaneiro cattle. Arch. Zootec. 5, 99–105.
Nei M., Roychoudhury A.K., 1974. Sampling variances of heterozygosity and genetic distance. Genetics 76, 379–390.
Radko A., Słota E., 2007. Polymorphism of 11 microsatellite DNA sequences used for parentage control in Holstein-Friesian bulls of black-and-white variety in Poland. Ann. Anim. Sci. 7, 189–196.
Radko A., Słota E., Marczyńska J., 2010. Usefulness of a supplementary set of microsatellite DNA markers for parentage testing in cattle. Pol. J. Vet. Sci. 13, 113–117.
Rejduch B., Kozubska-Sobocińska A., Radko A., Rychlik T., Słota E., 2004. The application of genetic markers for cell chimerism diagnosis in lambs. J. Anim. Breed. Genet. 121, 197–203.
Stevanovic J., Stanimirovic Z., Dimitrijevic V., Stojic V., Fratric N., Lazarevic M., 2009. Microsatellite DNA polymorphism and its usefulness for pedigree verification in Simmental cattle from Serbia. Acta Vet. (Beograd) 59(5–6), 621–631.
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