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Occurrence of entomopathogenic fungi in cultivated soils in Slovakia in the intensive agricultural system production

DOI: https://doi.org/10.24326/as.2025.5537
Przesłane: 30 kwietnia 2025
Opublikowane: 02.07.2025

Abstrakt

The aim of the study was to identify the types of entomopathogenic fungi (EPF) and to determine the intensity of their occurrence in intensively cultivated agricultural soils in the Danubian Lowland, Slovakia. Soil samples were collected on two dates (spring, autumn) from seven sites where the following crops were grown: soybean, barley, alfalfa, sugar beet, maize, maize with the addition of a biostimulator and from wasteland. The entomopathogenic fungi from individual soil samples were isolated using the isolation method on a selective medium. Entomopathogenic fungi were identified microscopically based on the morphology of their microstructures and the morphology of colonies, using standard keys. The number of EPF was presented in CFU g–1 of dry matter of soil. Both in spring and autumn, EPF belonging to three genera were identified: Beauveria, Metarhizium and Cordyceps. Analyzing the average density of infectious units of the identified genera of EPF in both study dates on arable land, it was found that fungi of the genus Metarhizium spp. formed more infectious units in the spring study date, whereas fungi of the genera Beauveria spp. and Cordyceps spp. formed more infectious units in the autumn study date, and these differences were statistically significant.

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