The aim of the present paper was the estimation of a possibility of gypsum utilization for limiting exchangeable aluminium in acid soil in relation to liming by CaCO3. The pot experiment with spring barley was carried out on acid sandy soil (pH 4.3) in 2002 – 2003. Mineral fertilization was applied in the following doses: N – 1 g, P – 0.2 g and K – 0.6 g per pot. CaCO3 and CaSO4 2H2O were applied in five different doses calculated by hydrolytic acidity (1 Hh, 2 Hh, 3 Hh, 4 Hh, 6 Hh). The yield of grain and the content of Ca, Mg, P and K in plant were determined. The soil reaction in 1 mol dm-3 KCl and exchangeable Al in soil were determined in soil samples by Sokołow method. The utilization of CaCO3 provided significant changes in soil reaction. The soil reaction increased with an increase of CaCO3 doses from pH 4.3 on the control object to pH 7.2 on the object with limed CaCO3 in doses calculated by six hydrolytic acidities. CaCO3 and CaSO4 2H2O utilization significantly limited the content of exchangeable aluminium in soil. The dose of CaCO3 calculated by 3-hydrolitic acidity totally neutralized exchangeable aluminium in soil. Gypsum utilization limited the content of exchangeable aluminium in soil. The content of exchangeable aluminium in the soil significantly decreased in relation to control object after application of the first doses of gypsum (calculated according to 1 hydrolitic acidity). Application of increasing doses of gypsum decreased the content of exchangeable aluminium in the soil. The content of exchangeable aluminium in gypsum fertilized soil in dose calculated by 6 hydrolitic acidity was equal to 0.06 g kg-1 of soil. No significant differences between yield of grain from the object limed CaCO3 and CaSO4 2H2O were observed. The plant from objects limed with CaCO3 and CaSO4 2H2O take a similar quantity of nutrients (Ca, Mg, P and K). The relationship between K:(Ca+Mg) was narrow in comparison to gypsum application than in condition of CaCO3 application.