Skip to main navigation menu Skip to main content Skip to site footer
ASPHC Baner

Vol. 17 No. 5 (2018)

Articles

NITROGEN METABOLISM IN CUCUMBER COTYLEONS AND LEAVES EXPOSED TO THE DROUGHT STRESS AND EXCESSIVE UV-B RADIATION

DOI: https://doi.org/10.24326/asphc.2018.5.3
Submitted: November 29, 2018
Published: 2018-11-29

Abstract

Plants absorb light energy for photosynthesis, and some amount of potentially damaging range of solar radiation, ultraviolet B. It accounts for less than 0.5% of the total solar radiation and the ambient current level of UV-B during the growth season provide 10 kJ·m–2 energy per day on the Earth’s surface. In the field conditions, increased UV-B radiation is often accompanied by drought, but negative effect of combined stresses is not so deleterious as the effect of one of them alone. We studied some changes in nitrogen me  deficit and UV-B radiation. The stresses generally decreased the biomass and total dry matter production. Combination of water deficit and UV-B activated a stress tolerance mechanism in cucumber seedlings. The NR activity and synthesis of UV-B absorbing compounds were induced. Protection against UV-B radiation can partially involve increased production of UV-B protective pigments – slightly increased the SPAD value in cucumber cotyledons and leaves was observed. The deleterious effect of combined stresses was weaker than their additive or individual effects.

References

  1. Alexieva, V., Ivanov, S., Sergiev, I., Karanov, E. (2003). Interaction between stresses. Bulg. J. Plant Physiol. Special issue, 1–17.
  2. Andrady, A., Aucamp, P.J., Bais, A.F., Ballare, C.L., Bjorn, L.O., Zepp, R.G. (2010). Environmental effects of ozone depletion and its interaction with climate change: progress report, 2009. Photochem. Photobiol. Sci., 9, 275–294.
  3. Arsimowicz-Jelonek, M., Floryszak-Wieczorek, J., Kubiś, J. (2009). Signal interaction between polyamines and nitric oxide intermediates cucumber adaptive responses to drought. J. Plant Growth Reg., 28, 177–186.
  4. Balakumar, T., Selvakumar, V., Sathiameena, K., Murugu Ilanchezhian, C., Paliwal, K. (1999). UV-B radiation mediated alterations in the nitrate assimilation pathway of crop plants. 1. Kinetic characteristics of nitrate reductase. Photosynthetica, 37, 459–467.
  5. Ballaré, C.L., Caldwell, M.M., Flint, S.D., Robinson, S.A., Bornman, J.F. (2011). Effects of solar ultraviolet radiation on terrestrial ecosystems. Patterns, mechanisms, and interactions with climate change. Photochem. Photobiol. Sci., 10, 226–241.
  6. Bandurska H., Niedziela, J., Chadzinikolau, T. (2013). Separate and combined response to water deficit and UV-B radiation. Plant Sci., 213, 98–105.
  7. Blum, A. (2014). Genomics for drought resistance – getting down to earth. Func. Plant Biol., 41, 1191–1198.
  8. Bornman, J.F., Barnes, P.W., Robinson, S.A., Ballare, C.L., Flint, S.D., Caldwell, M.M. (2015). Solar ultraviolet radiation and ozone depletion-driven climate change: effects on terrestrial ecosystems. Photochem. Photobiol. Sci., 14, 88–107.
  9. Campbel, W.H. (1999). Nitrate reductase structure, function and regulation: Bridging the gap between biochemistry and physiology. Annu. Rev. Plant Physiol. Mol. Biol., 50, 277–303.
  10. Canovas, F.M., Avila, C., Canton, F.R., Canas, R.A., de la Torre, F. (2007). Ammonium assimilation and amino acid metabolism in conifers. J. Exp. Bot., 58, 2307–2318.
  11. Cataldo, D.A., Maroon, M., Schrader, L.E., Youngs, V.L. (1975). Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid. Commun. Soil Sci. Plant. Anal., 6, 71–80.
  12. Eisinger, W., Swartz, T.E., Bogomolni, R.A., Taiz, L. (2000). The ultraviolet action spectrum for stomatal opening in broad bean. Plant Physiol., 122, 99–106.
  13. Favory, J.J, Stec, A., Gruber, H., Rizzini, L., Oravecz, A., Funk, M., Albert, A., Cloix, C., Jenkins, G.I., Oakeley, E.J., Seidlitz, H.K., Nagy, F., Ulm., R. (2009). Interaction of COP1 and UVR8 regulates UV‐B‐induced photomorphogenesis and stress acclimation in Arabidopsis. EMBO J., 28, 591–601.
  14. Feng, H., Li, S., Xue, L., An, L., Wang X., (2007). The interactive effects of enhanced UV-B radiation and soil drought on spring wheat. South Afr. J. Bot., 73, 429–434.
  15. Frohnmayer, H., Steiger, D. (2003). Ultraviolet-B radiation-mediated responses in plants. Balancing damage and protection. Plant Physiol., 133, 1420–1428.
  16. Ghisi, R.I., Trentin, A.R., Masi, A., Ferretti, M. (2002). Carbon and nitrogen metabolism in barley plants exposed to UV-B radiation. Physiol. Plant., 116, 200–205.
  17. Gupta, K.J., Bauwe, H., Mur, L.A.J. (2011). Nitric oxide, nitrate reductase and UV-B tolerance. Tree Physiol., 31, 795–797.
  18. Gwynn-Jones, D. (2001). Short-term impact of enhanced UV-B radiation on photo-assimilate allocation and metabolism: a possible interpretation for time-dependent inhibition of growth. Plant Ecol., 154, 67–73.
  19. Javadmanesh, S., Rahmani, F., Pourakbar, L. (2012). UVB radiation, soil salinity, drought stress and their current effects on some physiological parameters in maize plant. Am.-Euras. J. Toxicol. Sci., 4, 154–164.
  20. Jaworski, E.G. (1971). Nitrate reductase assay in intact plant tissues. Biochem. Biophys. Res. Commun., 43, 1274–1279.
  21. Krywult, M., Karolak, A., Bytnerowicz, A. (1996). Nitrate reductase activity as an indicator of Ponderosa pine response to atmospheric nitrogen deposition in San Barnardino Mountains. Environ. Poll., 93, 141–146.
  22. Krywult, M., Smykla, J., Kinnunen, H., Martz, F., Sutinen, M-L., Lakkala, K., Turunen, M. (2008). Influence of solar UV-B radiation on nitrogen metabolism in needles of Scots pine (Pinus silvestris L.). Environ. Poll., 156, 1105–1111.
  23. Krywult, M., Turunen, M., Sutinen, M.-L., Derome, K., Norokorpi, Y. (2002). Nitrate reductase activity in some subarctic species and UV-B influence in the foliage of Betula pendula Roth. Seedlings. Sci. Total Environ., 284, 149–155.
  24. Kubiś, J., Rybus-Zając, M. (2008). Drought and enhanced UV-B radiation differentially alter the antioxidant system in cucumber leaves. Acta Biol. Cracov. ser. Bot., 50, 35–41.
  25. Kubiś, J., Floryszak-Wieczorek, J., Arsimowicz-Jelonek, M. (2014). Polyamines induce adaptive responses in water deficit stressed cucumber roots. J. Plant Res., 127, 151–158.
  26. McKenzie, R.L., Björn, L.O., Bais, A., Ilyas, M. (2003). Changes in biologically active ultraviolet radiation reaching the Earth’s surface. Photochem. Photobiol. Sci., 2, 5–15.
  27. Mittler R., 2006. Abiotic stress, the field environment and stress combination. Trends Plant Sci., 11, 15–19.
  28. Norby, R.J., Weerasuriya, Y., Hanson, P.J. (1989). Induction of nitrate reductase activity in red spruce needles by NO2 and HNO3 vapor. Can. J. For. Res., 19, 889–896.
  29. Quaggiotti, S., Trentin, A.R., Vecchia, F.D., Ghisi, R. (2004). Response of maize (Zea mays L.) nitrate reductase to UV-B radiation. Plant Sci., 167, 107–116.
  30. Rajendiran, K., Ramanujam, M.P. (2006). Interactive effects of UV-B irradiation and triadimefon on nodulation and nitrogen metabolism in Vigna radiata plants. Biol. Plant., 50, 709–712.
  31. Rybus-Zając, M., Kubiś, J. (2010). Effect of UV-B radiation on antioxidative enzyme activity in cucumber cotyledons. Acta Biol. Cracov. ser. Bot., 52, 19, 97–102.
  32. Rybus-Zając, M., Korbas, A., Kubiś, J. (2017). The effect of drought stress and excessive UV-B radiation on the membrane status in cucumber cotyledons and leaves. EJPAU 20(4), 7.
  33. Scharf, P.C., Brouder, M.S., Hoeft, R.G. (2006). Chlorophyll meter readings can predict nitrogen need and yield response of corn in the North-Central USA. Agron. J., 98, 655–665.
  34. Sudaroli Sudha, J., Rajendiran, K. (2013). Effect of elevated UV-B irradiation on the nodulation and nitrogen metabolism in Sesbania grandiflora (L.). Int. J. Sci. Nat., 4, 664–667.
  35. Sullivan, J.H., Teramura, A.H. (1990). Field study of the interaction between solar ultraviolet-B radiation abd drought on photosynthesis and growth in soybean. Plant Physiol., 92, 141–146.
  36. Tuteja, N., Singh, M.B., Misra, M.K., Bhalla, P.L., Tuteja, R. (2001). Molecular mechanisms of DNA damage and repair: progress in plants. Crit. Rev. Biochem. Mol. Biol., 36, 337–397.
  37. Ulm, R., Jenkins G.I. 2015. Q&A: How do plants sense and respond to UV-B radiation? BMC Biology, 13, 45, 1–7.
  38. UNEP, 2002. Executive summary. Final of UNEP/WMO Scientific Assessment of Ozone Depletion: 2002. Prepared by the Scientific Assessment Panel of the Montreal Protocol on Substances that Deplete the Ozone Layer. UNEP, Nairobi (released 23 August 2002).
  39. Valliyodan, B., Nguyen, H.T., (2006). Understanding regulatory networks and engineering for enhanced drought tolerance in plants. Curr. Opin. Plant Biol., 9, 1–7.
  40. Van de Staaij, J.W.M., Ernst, W.H.O., Hakvort, H.V.J., Rozema, J. (1995). Ultraviolet B (290–320 nm) absorbing pigments in the leaves of Silene vulgaris: their role in UV-B tolerance. J. Plant Physiol., 147, 75–80.
  41. Singh, V.P. Singh, S., Prasad, S.M., Parihar, P. (2017). UV‐B radiation: from environmental stressor to regulator of plant growth.John Wiley & Sons, Oxford.
  42. Vijayalakshmi, R., Rajendiran, K. (2014). Impact of ultraviolet-B radiation on nodulation and nitrogen metabolism in Cyamopsis tetragonoloba (L.) Taub. Var. PNB. Inter. J. Geol., 4, 78–82.
  43. Weatherley, P.E. (1950). Studies in water relations of the cotton plants. I. The field measurement of water deficits in leaves. New Phytol., 49, 81–97.
  44. Zlatev, Z.S., Lidon, F.J.C., Kaimakanova, M. (2012). Plant physiological responses to UV-B radiation. Emir. J. Food Agric., 24, 481–501.
  45. Zhang, M., Dong, J.F., Jin, H.H., Sun, L.N., Xu, M.J. (2011). Ultraviolet-B-induced flavonoid accumulation in Betula pendula leaves is dependent upon nitrate reductase-mediated nitric oxide signalling. Tree Physiol., 31, 798–807.

Downloads

Download data is not yet available.

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.