Skip to main navigation menu Skip to main content Skip to site footer
ASPHC Baner

Vol. 20 No. 5 (2021)

Articles

The influence of exogenous gibberellic acid (GA3) and 24-epibrassinolide (24-EpiBL) on seed germination and the expression of genes involved in GA and BR synthesis/signalling in pepper (Capsicum annuum L.)

DOI: https://doi.org/10.24326/asphc.2021.5.2
Submitted: July 28, 2020
Published: 2021-10-29

Abstract

Gibberellins (GAs) and brassinosteroids (BRs) are the plant hormones involved in various physiological processes including seed germination. In this study, the effects of exogenous gibberellic acid (GA3) and 24-epibrassinolide (24-EpiBL) treatments on the expression of key genes involved in GA and BR syntheis/signalling during seed germination were investigated in pepper (Capsicum annuum L).

The expressions of BES1 and BRI1 involved in BR synthesis/signalling pathway as well as GA3OX1 and GA20OX1 associated with gibberellic acid biosynthesis in plants were determined. Exogenous GA3 treatments increased BES1 expression and the highest increase was determined with 10⁻⁸ M BR + 100 µM GA3 (P<0.05).  On the contrary, the expression of BRI1 gene was significantly decreased by 10-8 M BR + 100 µM GA3 (P<0.05). The expression of GA3OX1 gene was induced with BR and GA3 treatments (P<0.05). GA20OX1 gene expression was generally higher compared to the expression of GA3OX1 and significantly increased by the GA3 treatments. Our findings are expected to bring an insight to the influence of BRs during seed germination together with the expression of associated genes.

References

  1. Bai, M.Y., Shang, J.X., Oh, E., Fan, M., Bai, Y., Zentella, R., Sun, T., Wang, Z.Y. (2012). Brassinosteroid, gibberellin and phytochrome impinge on a common transcription module in Arabidopsis. Nat. Cell Biol., 14, 810–817. https://doi.org/10.1038/ncb2546
  2. Cheon, J., Fujioka, S., Dilkes, B.P., Choe, S. (2013). Brassinosteroids regulate plant growth through distinct signaling pathways in Selaginella and Arabidopsis. PlosOne, 8(12), e81938. https://doi.org/10.1371/journal.pone.0081938
  3. Chung, Y., Choe, S. (2013). The regulation of brassinosteroid biosynthesis in Arabidopsis. Critic. Rev. Plant Sci., 32(6), 396–410. https://doi.org/10.1080/07352689.2013.797856
  4. Davière, J.M., Achard, P. (2015). A pivotal role of DELLAs in regulating multiple hormone signals. Mol. Plant, 9(1), 10–20. https://doi.org/10.1016/j.molp.2015.09.011
  5. Da Silva, C.B., Marcos-Filho, J., Jourdan, P., Bennett, M.A. (2015). Performance of bell pepper seeds in response to drum priming with addition of 24-epibrassinolide. HortSci., 50(6), 873–878. https://doi.org/10.21273/HORTSCI.50.6.873
  6. Gudesblat, G.E., Russinova, E. (2011). Plants grow on brassinosteroids. Curr. Opin. Plant Biol., 14(5), 530–537. https://doi.org/10.1016/j.pbi.2011.05.004
  7. Hategan, L., Godza, B., Kozma‑Bognar, L., Bishop, G.J., Szekeres, M. (2014). Differential expression of the brassinosteroid receptor‑encoding BRI1 gene in Arabidopsis. Planta, 239, 989–1001. https://doi.org/10.1007/s00425-014-2031-4
  8. ISTA (2017). International rules for seed testing, International Seed Testing Association, Bassersdorf, Switzerland.
  9. Kucera, B., Cohn, M.A., Leubner-Metzger, G. (2005). Plant hormone interactions during seed dormancy release and germination. Seed Sci. Res., 15(4), 281–307. https://doi.org/10.1079/SSR2005218
  10. Li, J., Chory, J. (1997). A putative leucine rich repeat receptor kinase involved in brassinosteroid signal transduction. Cell, 90(5), 929–938. https://doi.org/10.1016/s0092-8674(00)80357-8
  11. Li, Q.F., He, J.X. (2013). Mechanisms of signaling crosstalk between brassinosteroids and gibberellins. Plant Signal Behav., 8(7), e24686. https://doi.org/10.4161/psb.24686
  12. Li, Q., Lu, J., Yu, J.W., Zhang, C.Q., He, J.X., Liu, Q.Q. (2018). The brassinosteroid-regulated transcription factors BZR1/BES1 function as a coordinator in multisignal-regulated plant growth. Biochim. Biophys. Acta Gene Regul. Mech., 1861(6), 561–571. https://doi.org/10.1016/j.bbagrm.2018.04.003
  13. Livak, K.J., Schmittgen, T.D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods, 25, 402–408. https://doi.org/10.1006/meth.2001.1262
  14. Ma, H.Y., Zhao, D.D., Ning, Q.R. Wei, J.P., Li, Y., Wang, M-M., Liu, X-L., Jiang, C-J., Liang, Z-W. (2018). A Multi-year beneficial effect of seed priming with gibberellic acid-3 (GA3) on plant growth and production in a perennial grass, Leymus chinensis. Sci. Rep., 8(1), 13214. https://doi:10.1038/s41598-018-31471-w
  15. Miransari, M., Smith, D.L. (2014). Plant hormones and seed germination. Environ. Exp. Bot., 99, 110–121. https://doi.org/10.1016/j.envexpbot.2013.11.005
  16. Mitchum, M.G., Yamaguchi, S., Hanada, A., Kuwahara, A., Yoshioka, Y., Kato, T., Tabata, S., Kamiya, Y., Sun, T-P. (2006). Distinct and overlapping roles of two gibberellin 3-oxidases in Arabidopsis development. Plant J., 45(5), 804–818. https://doi:10.1111/j.1365-313X.2005.02642.x
  17. Ross, J.J., Quittenden, J.L. (2016). Interactions Between Brassinosteroids and Gibberellins: Synthesis or Signaling? Letter to the editor. Plant Cell, 28, 829–832. https://doi.org/10.1105/tpc.15.00917
  18. Steber, C.M., McCourt, P. (2001). A role for brassinosteroids in germination in Arabidopsis. Plant Physiol., 125(2), 763–769. https://doi.org/10.1104/pp.125.2.763
  19. Unterholzner, S.J., Rozhon, W., Papacek, M., Ciomas, J., Lange, T., Kugler, K.G., Mayer, K.F., Sieberer, T., Poppenberger, B. (2015). Brassinosteroids are master regulators of gibberellin biosynthesis in Arabidopsis. Plant Cell, 27(8), 2261–2272. https://doi.org/10.1105/tpc.15.00433
  20. Qin, X., Liu, J.H., Zhao, W.S., Chen, X.J., Guo, Z.J., Peng, Y.L. (2013). Gibberellin 20-oxidase gene OsGA20OX3 regulates plant stature and disease development in rice. Mol. Plant Microbe Interact., 26(2), 227–323. https://doi.org/10.1094/MPMI-05-12-0138-R
  21. Wang, W., Bai, M-Y., Wang, Z-Y. (2014).The brassinosteroid signalling network – a paradigm of signal integration. Curr. Opin. Plant Biol., 21, 147–153. https://doi.org/10.1016/j.pbi.2014.07.012
  22. Wang, Z-Y., Nakano, T., Gendron, J., He, J., Chen, M., Vafeados, D., Yang, Y., Fujioka, S., Yoshida, S., Asami, T., Chory, J. (2002). Nuclear-localized BZR1 mediates brassinosteroid-induced growth and feedback suppression of brassinosteroid biosynthesis. Dev. Cell, 2(4), 505–513. https://doi.org/10.1016/S1534-5807(02)00153-3
  23. Yamaguchi, S., Kamiya, Y. (2000). Gibberellin Biosynthesis: Its Regulation by Endogenous and Environmental Signals. Plant Cell Physiol., 41(3), 251–257. https://doi.org/10.1093/pcp/41.3.251

Downloads

Download data is not yet available.

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.