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ASPHC Baner

Vol. 19 No. 5 (2020)

Articles

Incidence of viruses in cloves and bulbils of garlic ecotypes in Croatia

DOI: https://doi.org/10.24326/asphc.2020.5.10
Submitted: April 5, 2019
Published: 2020-10-30

Abstract

In a survey on the sanitary status of garlic collection performed in 2016 at the Institute of Agriculture and Tourism in Poreč (Croatia), 24 ecotypes and cultivars were tested by DAS-ELISA test for the presence of four main garlic viruses: Garlic common latent virus (GCLV), Shallot latent virus (SLV), Leek yellow stripe virus (LYSV) and Onion yellow dwarf virus (OYDV). The least frequent virus was SLV, whereas the most frequent was LYSV. In most ecotypes the infection of cloves was lower than infection of bulbils, while for SLV we found higher infection in cloves. Total virus infection of Istrian ecotypes was lower than infection of Dalmatian ones. Total incidence of viruses in Croatian garlic material ranged from 40.6% to 100.0%, depending on ecotype. In imported cultivar ‘Rose de Lautrec’ we found no infection with SLV. A high percentage of mixed infections in garlic cloves was also found and for double infections it ranged from 69% to 88%. Infection with all tested viruses was observed in 78% cloves. The most infected combination was double infections with potyviruses LYSV+OYDV. To the best of our knowledge, this is the first report of SLV infection in garlic in Croatia.

References

  1. Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L., Zurcher, E.J. (1996). Plant Viruses Online: Descriptions and List from the VIDE Database. Available: http://sdb.im.ac.cn/vide/sppindex.htm [date of access: 9.03.2018].
  2. Clark, M.F., Adams, A.N. (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol., 34, 475–483. DOI: 10.1099/0022-1317-34-3-475
  3. Conci, V.C., Canavelli, A., Lunello, P., Di Rienzo, J., Nome, S.F., Zumelzu, G., Italia, R. (2003). Yield losses associated with virus-infected garlic plants during five successive years. Plant Dis., 87(12), 1411–1415. Available: https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS.2003.87.12.1411 [date of access: 16.02.2017].
  4. Conci, V.C., Canavelli, A.E., Balzarini, M.G. (2010). The distribution of garlic viruses in leaves and bulbs during the first year of infection. J. Phytopathol., 158, 186–193. DOI: 10.1111/j.1439-0434.2009.01601.x
  5. Conci, V.C., Lunello, P., Buraschi, D., Italia, R.R., Nome, S.F. (2002). Variations of Leek yellow stripe virus concentration in garlic and its incidence in Argentina. Plant Dis., 86(10), 1085–1088.
  6. Croatian Bureau of Statistics (2017). 16-7. Production of Vegetables. In: Statistical Yearbook of the Republic of Croatia 2017, Ostroški, Lj. (ed.). Zagreb, 262–263.
  7. Dewan, C., Pearson, M.N., Scheffer, J.J.C. (1995). Natural field infection of garlic by garlic yellow streak virus in the Pukekohe area of New Zaeland and associated problems with the introduction of new garlic cultivars. N. Z. J. Crop Hortic. Sci., 23, 97–102.
  8. Dovas, C.I., Volvas, C. (2003). Viruses infecting Allium spp. in southern Italy. J. Plant Pathol., 85(2), 135.
  9. Dumičić, G., Čagalj, M., Urlić, B., Runjić, M., Goreta Ban, S. (2013). Komponente prinosa češnjaka (Allium sativum L.) [Yield components of garlic (Allium sativum L.)]. Zbornik radova 48. hrvatskog i 8. međunarodnog simpozija agronoma, 17th–22nd February 2013, Dubrovnik, Croatia, 349–352 [in Croatian].
  10. FAOSTAT – Food and Agricultural Organization of the United Nations (2016). Statistics. Available: http://faostat.fao.org [date of access: 14.03.2018].
  11. Fiume, F. (2005). Garlic. Available: http://francescofiume.altervista.org/Garlic.html [date of access: 11.09.2019.]
  12. Katis, N.I., Maliogka, V.I., Dovas, C.I. (2012). Virus of the genus Allium in the Mediterranean region. In: Advances in virus research, Loebenstein, G., Lecoq, H. (eds). vol. 84, Elsevier Inc., Amsterdam, 163–208.
  13. Keller, E.R.J., Senula, A. (2001). Progress in structuring and maintaining the garlic (Allium sativum) diversity for the European Genres Project. Acta Hortic., 555, 189–193. DOI: 10.17660/actahortic.2001.555.28
  14. Klukáčková, J., Navrátil, M., Duchoslav, M. (2007). Natural infection of garlic (Allium sativum L.) by viruses in the Czech Republic. J. Plant Dis. Prot., 114(3), 97–100. DOI: 10.1007/BF03356714
  15. Koch, M., Ta’anami, Z., Levi, S., Salomon, R. (1995). Testing garlic cloves and bublets for Onion yellow dwarf virus by ACP-ELISA. Phytoparasitica, 23(1), 27–29. DOI: 10.1007/BF02980393
  16. Lešić, R., Borošić, J., Buturac, I., Herak Ćustić, M., Poljak, M., Romić, D. (2002). Povrćarstvo. Zrinski, Čakovec [in Croatian].
  17. Lot, H., Chovelon, V., Souche, S., Delecolle, B. (1998). Effects of Onion yellow dwarf and Leek yellow stripe viruses on symptomatology and yield loss of three French garlic cultivars. Plant Dis., 8(12), 1381–1385. DOI: 10.1094/PDIS.1998.82.12.1381
  18. Lunello, P., Di Renzo, J., Conci, V.C. (2007). Yield loss in garlic caused by Leek yellow stripe virus Argentinian isolate. Plant Dis., 91(2), 153–158. DOI: 10.1094/PDIS-91-2-0153
  19. OEPP/EPPO (1995). EPPO Standards PP 2/4(2) Guidelines on good plant protection practice: Allium crops. Bulletin OEPP/EPPO Bulletin, 1–12.
  20. OEPP/EPPO (2015). EPPO Standard PM 7/125 (1) ELISA tests for viruses. Bulletin OEPP/EPPO Bulletin, 45(3), 445–449. DOI: 10.1111/epp.12259
  21. Parrano, L., Afunian, M., Pagliaccia, D., Douhan, G., Vidalakis, G. (2012). Characterization of viruses associated with garlic plants propagated from different reproductive tissues from Italy and other geographic regions. Phytopathol. Mediterr., 51(3), 549–565. DOI: 10.14601/Phytopathol_Mediterr-10479
  22. Perotto, M.C., Di Rienzo, J.A., Lanati, S., Panonto, S., Macchiavelli, R., Cafrune, E.E., Conci, V.C. (2014). Temporal and spatial spread of potyvirus infection and its relationship to aphid populations visiting garlic crops. Australas. Plant Pathol., 43, 623–630. DOI: 10.1007/s13313-014-0312-9
  23. Ramírez-Malagón, R., Pérez-Moreno, L., Borodanenko, A., Salinas-González, G.J., Ochoa-Alejo, N. (2006). Differential organ infection studies, potyvirus elimination and field performance of virus-free garlic plants produced by tissue culture. Plant Cell Tissue Organ Cult., 86, 103–110. DOI: 10.1007/s11240-006-9102-6
  24. Smékalová, K., Stavlíková, H., Dušek, K. (2010). Distribution of viruses in the garlic germplasm collection of the Czech Republic. J. Plant Pathol., 92(1), 273–274. DOI: 10.4454/jpp.v92i1.43
  25. Takaichi, M., Yamamoto, M., Nagakubo, T., Oeda, K. (2001). Mixed virus infections of garlic determined by Multivalent Polyclonal Antiserum and virus effects on disease yymptoms. Plant Dis., 85(1), 71–75. Available: https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS.2001.85.1.71 [date of access: 16.02.2017].
  26. Velásquez-Valle, R., Reveles-Hernandez, M., Amador-Ramirez, M.D. (2012). Viral distribution on asymptomatic onion (Allium cepa L.) plants. Rev. Mex. Cienc. Agric., 3(7), 1425–1434.
  27. Vončina, D., Ćurić, K., Fabek, S., Toth, N. (2016). First report of Onion yellow dwarf virus, Leek yellow stripe virus and Garlic common latent virus on Garlic in Croatia. Plant Dis., 100(3), 656. DOI: 10.1094/PDIS-08-15-0952-PDN
  28. Winiarczyk, K., Solarska, E., Sienkiewicz, W. (2014). Prevalence of infections with Onion yellow dwarf virus, Leek yellow stripe virus and Garlic common latent virus in plants from the genus Allium. Acta Sci. Pol. Hortorum Cultus., 13(3), 123–133.

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