Abstract
Allium aflatunense and Allium karataviense are valuable bulbous plants grown in a garden. Moreover, Allium aflatunense is one of the most decorative species for cut
flowers industry. These plants characterize with a low multiplication rate. Immature inflorescence stem sections and leaf bases isolated from ‘Allium aflatunense ‘Purple Sensation’ and Allium karataviense ‘Ivory Queen’ bulbs in October were used as source of explants for shoot regeneration. The use of a two – step sterilization procedure (2% Cl- – 30 min and 1% Cl- –15 min) gave much better results than a one – step (2% Cl- – 30 min). Inflorescence explants in inverted orientation were cultured on a Murashige and Skoog [1962] medium containing BA in concentration of 2 mg·dm-3 and NAA in concentration of 1 mg·dm-3.
Leaf bases placed in normal and inverted orientation were incubated on MS medium supplemented with: BA 2 mg·dm-3 plus NAA 1 mg·dm-3, BA 5 mg·dm-3 plus NAA 1 mg·dm-3 and TDZ 1 mg·dm-3 plus NAA 1 mg·dm-3. In subsequent experiments clumps consisting of 2 shoots were cultured on MS medium containing BA 0.5, 1, 2 mg·dm-3 with NAA 0.1 mg·dm-3 and BA 2 mg·dm-3 with IAA 0.1 mg·dm-3 for 6 weeks. A multiplication rate during four subcultures on medium with addition of BA 0.5 mg·dm-3 and NAA 0.1 mg·dm-3 was also examined. Explants from young inflorescences and from immature leaf bases were found effective to form shoots. The orientation of leaf explants did not have a significant effect on the number of regenerating shoots. 5.7 to 7.5 shoots were achieved for Allium aflatunense ‘Purple Sensation’ on studied media during 6 weeks of multiplication and 3.5 to 5.1 shoots for Allium karataviense ‘Ivory Queen’. Multiplication rate of 31.3 shoots for Allium aflatunense ‘Purple Sensation’ and 26.6 shoots for Allium karataviense ‘Ivory Queen’ was possible to obtain on medium supplemented with BA 0.5 mg·dm-3 and NAA 0.1 mg·dm-3 after 32 weeks of culture.
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