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ASPHC Baner

Vol. 21 No. 2 (2022)

Articles

Induction of adventitious buds and establishment of an efficient regeneration system in Polygonatum cyrtonema Hua

DOI: https://doi.org/10.24326/asphc.2022.2.5
Submitted: July 3, 2021
Published: 2022-04-29

Abstract

Polygonatum cyrtonema Hua is not only a widely used traditional Chinese medicine, but also processed into food and cosmetics. However, its large-scale cultivation and application are limited by the low reproduction rate for traditional artificial propagation technology. Therefore, the aim of this study was to establish an efficient and stable adventitious buds induction system for P. cyrtonema by using one bud rhizome segment as initial explants. The results showed that the optimal medium for the rhizome growth, adventitious buds occurrence and proliferation was MS medium supplemented with 3.0 mg·L–1 BA, 1.0 mg·L–1 NAA and 1.0 mg·L–1 KT, with a proliferation coefficient of 6.5. The best rooting medium was found to be half strength of MS medium with 0.5–1.0 mg·L–1 NAA. The survival rate was higher than 95%. Finally, an asexual rapid propagation system of P. cyrtonema was established, which can provide the technical basis for the development of nursery culture.

References

  1. Aloni, R. (2010). The induction of vascular tissues by auxin plant hormones. Springer, Dordrecht-Netherlands, 485–518. https://doi.org/10.1007/978-94-009-3585-3_19 DOI: https://doi.org/10.1007/978-1-4020-2686-7_22
  2. Beena, M.R., Martin, K.P., Kirti, P.B., Harihahan, M. (2003). Rapid in vitro propagation of medicinally important Ceropegia candelabrum. Plant Cell Tiss. Org., 72(3), 285–289. https://doi.org/10.1023/A:1022395809204 DOI: https://doi.org/10.1023/A:1022395809204
  3. Bisht, S., Bisht, N.S., Bhandari, S. (2012). In vitro micropropagation in Polygonatum verticillatum (L.) All. an important threatened medicinal herb of Northern India. Physiol. Mol. Biol. Pla., 18(1), 89–93. https://doi.org/10.1007/s12298-011-0091-5 DOI: https://doi.org/10.1007/s12298-011-0091-5
  4. Chen, C.M., Ertl, J.R., Leisner, S.M. (1985). Localization of cytokinin biosynthetic sites in pea plants and carrot roots. Plant Physiol., 78(3), 510–513. https://doi.org/10.1104/pp.78.3.510 DOI: https://doi.org/10.1104/pp.78.3.510
  5. Chinese Academy of Sciences China flora editorial board. (2000). Flora of China. Vol. 24. S Press, Bejing-China, 267.
  6. Chinese Pharmacopoeia Commission. (2020). Pharmacopoeia of the People’s Republic of China. Part I. CMS Press, Beijing-China, 319.
  7. Ding, Y.H., Zhao, R.N. (1991). An investigation of medicinal Polygonattum in Northwest China. China J. Chin. Mater. Med., 16, 202–204.
  8. Feng, H., Zhang, J.L., Wu, P., Li, X.Y. (2019). Optimization of low-sugar polygonatum jam production process by response surface methodology. J. Food Technol. Preserv., 3(2), 9–8. https://doi.org/10.22606/afse.2019.32001 DOI: https://doi.org/10.22606/afse.2019.32001
  9. Gonçalves, S., Escapa, A.L., Grevenstuk, T., Romano, A. (2008). An efficient in vitro propagation protocol for Pinguicula lusitanica, a rare insectivorous plant. Plant Cell Tiss. Org., 95, 239–243. https://doi.org/10.1007/s11240-008-9423-8 DOI: https://doi.org/10.1007/s11240-008-9423-8
  10. He, Y., Zhu, Y.Q., Xiao, B., Fu, S.H., Si, P.J. (2019). Study on tissue culture system of Polygonatum cyrtonema. China J. Chin. Mater. Med., 44, 2032–2037. https://doi.org/10.19540/j.cnki.cjcmm.20190301.010
  11. Li, N., Huang, H.Y., Zeng, B. (2020). Cluster bud induction of base stem and establishment of high efficiency regeneration system of Lycium ruthenicum. Chinese Trad. Herba Drugs, 51, 3545–3553. https://doi.org/10.7501/j.issn.0253-2670.2020.13.024
  12. Noshad, D., Miresmaili, S., Riseman, A., Ekramoddoullah, A. (2009). In vitro propagation of seven Daphne L. species. Plant Cell Tiss. Org., 96, 201–209. https://doi.org/10.1007/s11240-008-9476-8 DOI: https://doi.org/10.1007/s11240-008-9476-8
  13. Ramírez-Malagón, R., Borodanenko, A., Pérez-Moreno, L., Salas-Araiza, M.D., Nuñez-Palenius, H.G., Ochoa-Alejo, N. (2008). In vitro propagation of three Agave species used for liquor distillation and three for landscape. Plant Cell Tiss. Org., 94, 201–207. https://doi.org/10.1007/s11240-008-9405-x DOI: https://doi.org/10.1007/s11240-008-9405-x
  14. Sundberg, B., Little, C.H.A., Cui, K. (1991). Leve of endogenous indole-3-acetic acid in the stem of pinus sylvestris in relation to the seasonal variation of cambial activity. Plant Cell Environ., 14, 241–246. https://doi.org/10.1111/j.1365-3040.1991.tb01342.x DOI: https://doi.org/10.1111/j.1365-3040.1991.tb01342.x
  15. Tang, C., Yu, Y.., Qi, Q.L., Wu, X.D., Wang, J., Tang, S.A. (2019). Steroidal saponins from the rhizome of Polygonatum sibiricum. J. Asian Nat. Prod. Res., 21, 197–206. https://doi.org/10.1080/10286020.2018.1478815 DOI: https://doi.org/10.1080/10286020.2018.1478815
  16. Ti, W.G., Hu, L.Z., Gong, H.F., Jin, X.X., Liu, W.Z., Chen, H. (2016). Rapid propagation of Halocnemum strobilaceum using axillary bud cluster from assimilating branch. Plant Physiol. J., 52, 431–435. https://doi.org/10.13592/j.cnki.ppj.2015.0410
  17. Tiwari, T., Chaturvedi, P. (2018). Callus induction in Polygonatum verticillatum (L.) All.: an Astavarga medicinal herb. J. Pharmacogn. Phytochem., 7, 2671–2674.
  18. Torrey, J.G. (1972). On the initiation of organization in the root apex. In: Miller, M.W., Kuehnert, C.C., (eds). The dynamics of meristem cell populations: advances in experimental medicine and biology. Springer, Boston –New York, 1–13. DOI: https://doi.org/10.1007/978-1-4684-3207-7_1
  19. Van-Staden, J., Davey, J.E. (1979). The synthesis, transport and metabolism of endogenous cytokinins. Plant Cell Environ., 2, 93–106. https://doi.org/10.1111/j.1365-3040.1979.tb00780.x DOI: https://doi.org/10.1111/j.1365-3040.1979.tb00780.x
  20. Wang, H., Xiao, X.J., Tang, C., Ke, X.M., Zhao, S.S. (2021). Establishment of efficient regeneration system of Polygonatum cyrtonema Hua. Mol. Plant Breed., 6, 1–10. http://kns.cnki.net/kcms/detail/46.1068.S.20210422.1707.012.html.
  21. Wang, Y., Dong X., Huang H.Y., Wang, Y.Z. (2019). Establishment of efficient adventitious shoots induction system and ex vitro rooting in Vaccinium corymbosum (Ericaceae). Bot. Sci., 97, 180–191. https://doi.org/10.17129/botsci.2135 DOI: https://doi.org/10.17129/botsci.2135
  22. Xi, Y.K., Wang, Y., Zeng, B., Huang, H.Y., Yang, W.D. (2020). Callus induction and adventitious bud differentiation of Cyclocodon lancifolius (Roxb.) Kurz. Bot. Sci., 98, 534–544. https://doi.org/10.17129/botsci.2609 DOI: https://doi.org/10.17129/botsci.2609
  23. Xie, Z.W., Fan, C.S. (1996). Chinese traditional herbal medicine. Ph Press, Beijing-China, 800–802.
  24. Yelithao, K., Surayot, U., Park, W., Lee, S., Lee, D.H., You, S. (2019). Effect of sulfation and partial hydrolysis of polysaccharides from Polygonatum sibiricum on immune-enhancement. Int. J. Biol. Macromol., 122, 10–18. https://doi.org/10.1016/j.ijbiomac.2018.10.119 DOI: https://doi.org/10.1016/j.ijbiomac.2018.10.119
  25. Zhang, J., Wang, Y.Z., Yang, W.Z., Chine, Y.M.Q., Zhang, J.Y. (2019). Research progress in chemical constituents in plants of Polygonatum and their pharmacological effects. China J. Chinese Mater. Medica, 44, 1989–2008. https://doi.org/10.19540/j.cnki.cjcmm.20190222.006
  26. Zhao, D.L., Wang, X.Y., Lu, W., Zheng, G.C. (2003). Plant regeneration via organogenesis from adventitious bud explants of a medicinal herb species, Polygonatum Cyrtonema. In Vitro Cell Dev. Pl., 39, 24–27. https://doi.org/10.1079/IVP2002359 DOI: https://doi.org/10.1079/IVP2002359
  27. Zhao, H., Wang, Q.L., Hou, S.B., Chen, G. (2019). Chemical constituents from the rhizomes of Polygonatum sibiricum Red. and anti-inflammatory activity in RAW264.7 macrophage cells. Nat. Prod. Res., 33, 2359–2362. https://doi.org/10.1080/14786419.2018.1440220 DOI: https://doi.org/10.1080/14786419.2018.1440220
  28. Zhao, Q., Wu, C.F., Wang, W.G., Yuan, S., Bao, J.K., Chen, F. (2009). In vitro plantlet regeneration system from rhizomes and mannose-binding lectin analysis of Polygonatum cyrtonema Hua. Plant Cell. Tiss. Org., 99, 269–275. https://doi.org/10.1007/s11240-009-9600-4 DOI: https://doi.org/10.1007/s11240-009-9600-4

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