Skip to main navigation menu Skip to main content Skip to site footer
ASPHC Baner

ONLINE FIRST

Research paper

Response of rooting stem cuttings of sage (Salvia officinalis L.) for exogenous hormone applications in different growth media

DOI: https://doi.org/10.24326/asphc.2026.5588
Submitted: 19 August 2025
Published: 28.04.2026

Abstract

The objective of this study was to investigate the effects of different growth media and IBA doses on the growth parameters of Salvia officinalis L. plant. The study was conducted according to a factorial experimental design in randomized plots within the climate cabin, which has fully controlled conditions, located in the Department of Horticulture at Van Yüzüncü Yıl University. A total of 360 cuttings were used, with 10 cuttings representing each application. In the study, which was conducted with three replications, plants were grown in 200 mL pots. The cuttings used in the rooting study were obtained from medicinal sage (S. officinalis) plants in the Medicinal and Aromatic Plants Garden of Van Yüzüncü Yıl University, Faculty of Agriculture, in June 2023. The cuttings were brought to the laboratory, planted in 3 different media: perlite (100%); perlite + peat (1 : 1); peat (100%), and applying four different concentrations of hormones: 0 ppm; 250 ppm; 500 ppm; 1000 ppm IBA (indole-3-butyric acid). The trial ended 50 days following planting, and the necessary measurements were made. In this study, the highest growth performance were obtained in the perlite + peat growth medium, except for root length. Considering the hormone × growth media interactions, the highest fresh plant weight, dry plant weight, fresh root weight, and dry root weight were obtained as 3.549 g, 0.976 g, 4.351 g, and 0.644 g, respectively, in perlite + peat growth medium applied with 1000 mg kg-1 IBA. The highest plant length (17.617 cm) and rooting rate (80%) were also obtained with 1000 mg kg‒1 IBA application in peat (100%) and perlite (100%) growth media, respectively.

References

  1. Abdnezhad, R., Simbar, M., Sheikhan, Z. et al. (2019). Salvia officinalis reduces the severity of the premenstrual syndrome. Complement. Med. Res., 26(1), 39‒46. https://doi.org/10.1159/000490104
  2. Agatonović-Kustrin, S., Gegechkori, V., Kustrin, E. et al. (2022). The effect of lactic acid fermentation on extraction of phenolics and flavonoids from sage leaves. Appl. Sci, 12(19), 9959. https://doi.org/10.3390/app12199959
  3. Aljuboori, I.W., Mahmood, M.S., Al-Rihaymee, S.A. et al. (2024). Clinical effectiveness of Salvia officinalis in periodontitis: a split-mouth randomized controlled trial. Cureus, 16(4). https://doi.org/10.7759/cureus.58582
  4. Arthagama, I.D.M., Dana, I.M., Wiguna, P.P.K. (2021). Effect of various types of growing media and application of liquid organic fertilizer on the growth of Dendrobium orchids. Int. J. Biosci. Biotechnol., 8(2), 54‒61. https://doi.org/10.24843/IJBB.2021.v08.i02.p07
  5. Aytekin, R.İ., Akkamış, M., Bedir, M. et al. (2021). Effect of different growth media on germination and seedling quality of sage (Salvia officinalis L.) seeds. Mustafa Kemal Univ. J. Agric. Sci., 26(3), 610‒616. https://doi.org/10.37908/mkutbd.956193
  6. Çiçek, E., Ozel, A. (2021). Determination of suitable cutting type and IBA dose for seedling production in lavender (Lavandula angustifolia Mill.). Harran J. Agric. Food Sci., 25(2), 254‒264. https://doi.org/10.29050/harranziraat.827325
  7. Currey, C.J., Flax, N.J., Litvin, A.G., et al. (2019). Substrate volumetric water content controls growth and development of containerized culinary herbs. Agronomy, 9(11), 667.
  8. Do, H., Scherer, C. (2013). Compost as growing media component for salt-sensitive plants. Plant Soil Environ., 59(2), 85‒90. https://doi.org/10.17221/804/2012-PSE
  9. Erhan, M.K. (2020). The potential of Salvia officinalis as a suppressor of cell proliferation in animal feed and human nutrition: an experimental stud. Turk. J. Vet. Anim. Sci., 44(2), 244‒248.
  10. Fattorini, L., Veloccia, A., Rovere, F.D. et al. (2017). Indole-3-butyric acid promotes adventitious rooting in Arabidopsis thaliana thin cell layers by conversion into indole-3-acetic acid and stimulation of anthranilate synthase activity. BMC Plant Biol., 17(1), 121. https://doi.org/10.1186/s12870-017-1071-x
  11. Ferrarezi, R.S., Iersel, M.W.V., Testezlaf, R. (2016). Plant growth response of subirrigated salvia ‘Vista Red’ to increasing water levels at two substrates. Hortic. Bras., 34(2), 202‒209. https://doi.org/10.1590/s0102-053620160000200009
  12. Gur, E., Certin, M., Ozel, H.B. et al. (2021). The effect of hormone treatments on germination and seedling characters of sage (Salvia officinalis l.) seeds. Alinteri J. Agric. Sci., 36(2), 62‒69. https://doi.org/10.47059/alinteri/v36i2/ajas21115
  13. Heydarpour, S., Sharifipour, F., Heydarpour, F. (2023). Effect of Salvia officinalis scent on postmenopausal women’s sexual function and satisfaction: a randomized controlled trial. BMC Women's Health, 23(1), 442. https://doi.org/10.1186/s12905-023-02605-8
  14. Li, Z., Ma, Y., Yin, W. et al. (2019). Exploring vegetative propagation techniques for the threatened Pteroceltis tatarinowii maxim using stem cuttings. HortScience, 54(4), 721‒724. https://doi.org/10.21273/hortsci13729-18
  15. Logsdon, S.D., Sauer, P.A. (2016). Nutrient leaching when compost is part of plant growth media. Compost Sci. Util., 24(4), 238‒245.
  16. Londra, P.A. (2010). Simultaneous determination of water retention curve and unsaturated hydraulic conductivity of substrates using a steady-state laboratory method. HortScience, 45(7), 1106‒1112.
  17. Martini, A.N., Vlachou, G., Papafotiou, Μ. (2022). Effect of explant origin and medium plant growth regulators on in vitro shoot proliferation and rooting of salvia tomentosa, a native sage of the northeastern mediterranean basin. Agronomy, 12(8), 1889. https://doi.org/10.3390/agronomy12081889
  18. Maghdouri, M., Ghasemnezhad, M., Rabiei, B. et al. (2021). Optimizing seed germination and seedling growth in different kiwifruit genotypes. Horticulturae, 7(9), 314. https://doi.org/10.3390/horticulturae7090314
  19. Papafotiou, Μ., Kargas, G., Lytra, I. (2005). Olive-mill waste compost as a growth medium component for foliage potted plants. HortScience, 40(6), 1746‒1750. https://doi.org/10.21273/hortsci.40.6.1746
  20. Pop, A.V., Tofană, M., Socaci, S.A. et al. (2016). Determination of antioxidant capacity and antimicrobial activity of selected Salvia species. Bull. UASVM Food Sci. Technol, 73. https://doi.org/10.15835/buasvmcn-fst:11965
  21. Rodrigues, M.R.A., Kanazawa, L.K.S., das Neves, T.L.M. et al. (2012). Antinociceptive and anti-inflammatory potential of extract and isolated compounds from the leaves of Salvia officinalis in mice. J. Ethnopharmacol., 139(2), 519‒526. https://doi.org/10.1016/j.jep.2011.11.042
  22. Salamat, S.S., Hassan, M.A., Shirai, Y. et al. (2019). Application of compost in mixed media improved oil palm nursery’s secondary root structure thereby reducing the fertilizer requirement for growth. J. Mol. Biol. Biotechnol., 27(3), 39‒49.
  23. Sari, F.O.S.O., Rugayah, R., Ginting, Y.C.Y.C. (2014). Pengaruh konsentrasi IBA (Indole Butyric Acid) dan jenis media tanam terhadap pertumbuhan bibit nanas (Ananas comosus [L.] Merr) asal tunas mahkota. J. Agrotek Tropika, 2(1), 43‒48 [in Indonesian].
  24. Sonmez, C., Bayram, E. (2017). The influence of different water and nitrogen applications on some yield parameters and antioxidant activity in sage (Salvia officinalis L.). Turk. J. Field Crops, 22(1), 96‒103.
  25. Tan, U. (2025). Application of indole-3-butyric acid (IBA) enhances agronomic, physiological and antioxidant traits of Salvia fruticosa under saline conditions: a practical approach. PeerJ, 13, e18846. https://doi.org/10.7717/peerj.18846
  26. Xu, R. (2022). Salvia officinalis-based green-mediated vanadium nanoparticles: describing a modern chemotherapeutic drug for the treatment of colorectal carcinoma. Authorea Preprints. https://doi.org/10.22541/au.166857482.26607149/v1
  27. Yoo, Y.K., Kim, I.K., Roh, M.S. et al. (2017). Growth, flowering, and nutrient composition of salvia grown in peat moss media containing pellets processed with poultry feather fibers at different mixing ratios. Hortic. Sci. Technol., 35(3), 289‒299. https://doi.org/10.12972/kjhst.20170032

Downloads

Download data is not yet available.

Similar Articles

<< < 33 34 35 36 37 38 39 40 41 42 > >> 

You may also start an advanced similarity search for this article.