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ASPHC Baner

Vol. 25 No. 3 (2026)

Research paper

Influence of mycorrhizal fungi on plant growth and rhizosphere soil microbiome of tomato (Solanum lycopersicum L.)

DOI: https://doi.org/10.24326/asphc.2026.5608
Submitted: 30 September 2025
Published: 22.06.2026

Abstract

Mycorrhizal fungi increase plant resistance to stress factors such as drought, high or low temperatures, acidification, soil contamination with heavy metals, and the presence of soil pathogens. The aim of the study was to determine the effect of mycorrhizal inoculum on plant growth and the microbiome of the rhizosphere soil of tomato. The experiment was established in the greenhouse of the Felin Experimental Farm at the University of Life Sciences in Lublin. The research material consisted of tomato plants (Solanum lycopersicum L.) Lubań cv. The mycorrhizal inoculum Endo-VAM from Mykoflor (Końskowola, Poland) was used for mycorrhization of tomato seedlings. It contains spores and live mycelium of the following species: Glomus intraradices, G. mosseae, G. claroideum, G. etunicatum, Gigaspora margerita, and Entrophospora spp. Non-mycorrhized tomato seedlings served as a control. Six weeks after the application of the mycorrhizal inoculum, mycological analysis of tomato roots and microbiological analysis of rhizosphere soil were performed, and plant height, stem base diameter, fresh and dry root weight were determined. The roots of mycorrhized plants were found to be more abundantly colonized by fungi, including antagonistic species, than the roots of non-mycorrhized plants. The total number of bacteria, including Pseudomonas and Bacillus genera, and the total number of fungi isolated from the rhizosphere of mycorrhized plants were significantly lower than in the rhizosphere of control plants. Analysis of morphological parameters of tomato plants demonstrated a beneficial effect of the mycorrhizal inoculum on the growth of fresh and dry root weight, but did not demonstrate a significant effect of mycorrhiza on plant height or tomato stem base diameter.

References

  1. Al-Askar, A.A., Rashad, Y.M. (2010). Arbuscular mycorrhizal fungi: a biocontrol agent against common bean Fusarium root disease. Plant Pathol. J., 9(1), 31–38. https://doi.org/10.3923/ppj.2010.31.38 DOI: https://doi.org/10.3923/ppj.2010.31.38
  2. Al-Hmoud, G., Al.-Momany, A. (2015). Effect of four mycorrhizal products on Fusarium root rot on different vegetable crops. J. Plant Pathol. Microbiol., 6(2), 1–5. http://dx.doi.org/10.4172/2157-7471.1000255 DOI: https://doi.org/10.4172/2157-7471.1000255
  3. Amer, M.A., Abou-el-Seoud, I.I. (2008). Mycorrhizal fungi and Trichoderma harzianum as biocontrol agents for suppression of Rhizoctonia solani damping-of disease of tomato. Commun. Agric. Appl. Biol. Sci., 73(2), 217– 232.
  4. Balestrini, R., Bonfante, P. (2014). Cell wall remodeling in mycorrhizal symbiosis: a way towards biotrophism. Front. Plant Sci., 5, 237. https://doi.org/10.3389/fpls.2014.00237 DOI: https://doi.org/10.3389/fpls.2014.00237
  5. Basirus, S., Mwanza, H.P., Hijri, M. (2021). Analysis of arbuscular mycorrhizal fungal inoculant benchmarks. Microorganisms, 9(1), 81. https://doi.org/10.3390/microorganisms9010081 DOI: https://doi.org/10.3390/microorganisms9010081
  6. Basyal, B., Emery, S.M. (2021). An arbuscular mycorrhizal fungus alters switchgrass growth, root architecture, and cell wall chemistry across a soil moisture gradient. Mycorrhiza, 31, 251–258. https://doi.org/10.1007/s00572-020-00992-6 DOI: https://doi.org/10.1007/s00572-020-00992-6
  7. Błaszczyk, L., Siwulski, M., Sobieralski, K. et al. (2014). Trichoderma spp. – application and prospects for use in organic farming and industry. J. Plant Prot. Res.. 54(4), 309–317. DOI: https://doi.org/10.2478/jppr-2014-0047
  8. Bücking, H., Abubaker, J., Govindarajulu, M., et al. (2008). Root exudates stimulate the uptake and metabolism of organic carbon in germinating spores of Glomus intraradices. New Phytol., 180, 684–695. https://doi.org/10.1111/j.1469-8137.2008.02590.x DOI: https://doi.org/10.1111/j.1469-8137.2008.02590.x
  9. Caccia, M., Marro, N., Novák, V. et al. (2024). Divergent colonization traits, convergent benefits: different species of arbuscular mycorrhizal fungi alleviate Meloidogyne incognita damage in tomato. Mycorrhiza, 34, 145–158. https://doi.org/10.1007/s00572-024-01139-7 DOI: https://doi.org/10.1007/s00572-024-01139-7
  10. Caradonia, F., Francia, E., Morcia, C. et al. (2019). Arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria avoid processing tomato leaf damage during chilling stress. Agronomy, 9(6), 299. https://doi.org/10.3390/agronomy9060299 DOI: https://doi.org/10.3390/agronomy9060299
  11. Chen, X., Lu, Y., Liu, X. et al. (2025). Trichoderma: dual roles in biocontrol and plant growth promotion. Microorganisms, 13(8), 1840. https://doi.org/10.3390/microorganisms13081840 DOI: https://doi.org/10.3390/microorganisms13081840
  12. Chitarra, W., Pagliarani, C., Maserti, B. et al. (2016). Insights on the impact of arbuscular mycorrhizal symbiosis on tomato tolerance to water stress. Plant Physiol., 171(2), 1009–1023. https://doi.org/10.1104/pp.16.00307 DOI: https://doi.org/10.1104/pp.16.00307
  13. Delaeter, M., Magnin-Robert, M., Randoux, B. et al. (2024). Arbuscular mycorrhizal fungi as biostimulant and biocontrol agents: a review. Microorganisms, 12(7), 1281. https://doi.org/10.3390/microorganisms12071281 DOI: https://doi.org/10.3390/microorganisms12071281
  14. Derkowska, E., Sas-Paszt, L., Dyki, B. et al. (2015). Assessment of mycorrhizal frequency in the roots of fruit plants using different dyes. Adv. Microbiol., 5(1), 54–64. https://doi.org/10.4236/AIM.2015.51006 DOI: https://doi.org/10.4236/aim.2015.51006
  15. Derkowska, E., Sas-Paszt, L., Sumorok, B. et al. (2024). The influence of bioproducts on mycorrhizal occurrence in vegetable roots. Hortic. Sci., 51(4), 327–340. https://doi.org/10.17221/69/2023-HORTSCI DOI: https://doi.org/10.17221/69/2023-HORTSCI
  16. Felföldi, Z., Vidican, R., Stoian, V. et al. (2022). Arbuscular mycorrhizal fungi and fertilization influence yield, growth and root colonization of different tomato genotype. Plants, 11(13), 1743. https://doi.org/10.3390/plants11131743 DOI: https://doi.org/10.3390/plants11131743
  17. Fiorilli, V., Martínez-Medina, A., Pozo, M.J. et al. (2024). Plant immunity modulation in arbuscular mycorrhizal symbiosis and its impact on pathogens and pests. Ann. Rev. Phytopathol., 62, 127–156. https://doi.org/10.1146/annurev-phyto-121423-042014 DOI: https://doi.org/10.1146/annurev-phyto-121423-042014
  18. Fracasso, A., Telò, L., Lanfranco, L. et al. (2020). Physiological beneficial effect of Rhizophagus intraradices inoculation on tomato plant yield under water deficit conditions. Agronomy, 10(1), 71. https://doi.org/10.3390/agronomy10010071 DOI: https://doi.org/10.3390/agronomy10010071
  19. Fujita, M., Kusajima, M., Fukagawa, M. et al. (2022). Response of tomatoes primed by mycorrhizal colonization to virulent and avirulent bacterial pathogens. Sci. Rep., 12, 4686. https://doi.org/10.1038/s41598-022-08395-7 DOI: https://doi.org/10.1038/s41598-022-08395-7
  20. Gallou, A., Mosquera H.P.L., Cranenbrouck, S. et al. (2011). Mycorrhiza induced resistance in potato plantlets challenged by Phytophthora infestans. Physiol. Mol. Plant Pathol., 76(1), 20–26. https://doi.org/10.1016/j.pmpp.2011.06.005 DOI: https://doi.org/10.1016/j.pmpp.2011.06.005
  21. Głuszek, S., Sas-Paszt, L., Sumorok, B. et al. (2008). Wpływ mikoryzy na wzrost i plonowanie roślin ogrodniczych. Post. Nauk Rol., 60(6), 11–22.
  22. Hart, M.M., Reader, R.J. (2002). Taxonomic basis for variation in the colonization strategy of arbuscular mycorrhizal fungi. New Phytol., 153(2), 335–344. https://doi.org/10.1046/j.0028-646X.2001.00312.x DOI: https://doi.org/10.1046/j.0028-646X.2001.00312.x
  23. https://www2.dijon.inrae.fr/mychintec/Mycocalc-prg/download.html [date of access: 23.02.2026]
  24. Jamiołkowska, A. (2007). Effect of field pea (Pisum arvense L.) as cover plant on health of under-ground part of field tomato. Veg. Crops Res. Bull., 67, 71–79. https://doi.org/10.2478/v10032-007-0032-6 DOI: https://doi.org/10.2478/v10032-007-0032-6
  25. Jamiołkowska, A., Księżniak, A., Gałązka, A. et al. (2018). Impact of abiotic factors on development of the community of arbuscular mycorrhizal fungi in the soil: a review. Int. Agrophys., 32, 133–140. https://doi.org/10.1515/intag-2016-0090 DOI: https://doi.org/10.1515/intag-2016-0090
  26. Jamiołkowska, A., Thanoon, A.H., Skwaryło, B. et al. (2020a). Mycorrhizal inoculation as an alternative for the ecological production of tomato (Lycopersicon esculentum Mill.). Int. Agrophys., 34(2), 253–264. https://doi.org/10.31545/intagr/118196 DOI: https://doi.org/10.31545/intagr/118196
  27. Jamiołkowska, A., Skwaryło-Bednarz, B., Patkowska, E. et al. (2020b). Effect of mycorrhizal inoculation and irrigation on biological properties of sweet pepper rhizosphere in organic field cultivation. Agronomy, 10(11), 1693. https://doi.org/10.3390/agronomy10111693 DOI: https://doi.org/10.3390/agronomy10111693
  28. Jaroszuk-Ściseł, J., Kurek, E., Rodzik, B. et al. (2009). Interactions between rye (Secale cereale) root border cells (RBCs) and pathogenic and nonpathogenic rhizosphere strains of Fusarium culmorum. Mycol. Res., 113, 1053–1061. https://doi.org/10.1016/j.mycres.2009.07.001 DOI: https://doi.org/10.1016/j.mycres.2009.07.001
  29. Jung, S.C., Martinez-Medina, A., Lopez-Raez, J.A. et al. (2012). Mycorrhiza-induced resistance and priming of plant defenses. J. Chem. Ecol., 38, 651–664. https://doi.org/10.1007/s10886-012-0134-6 DOI: https://doi.org/10.1007/s10886-012-0134-6
  30. Kaboré, K., Konaté, K., Sanou, A. et al. (2022). Tomato by-products, a source of nutrients for the prevention and reduction of malnutrition. Nutrients, 14(14), 2871. https://doi.org/10.3390/nu14142871 DOI: https://doi.org/10.3390/nu14142871
  31. Lee, S.H., Won, H.J., Ban, S. et al. (2023). Tomato fruit growth and nutrient accumulation in response to blue and red light treatments during the reproductive growth stage. Horticulturae, 9(10), 1113. https://doi.org/10.3390/horticulturae9101113 DOI: https://doi.org/10.3390/horticulturae9101113
  32. Leventis, G., Tsiknia, M., Feka, M. et al. (2021). Arbuscular mycorrhizal fungi enhance growth of tomato under normal and drought conditions, via different water regulation mechanisms. Rhizosphere, 19, 100394. https://doi.org/10.1016/j.rhisph.2021.100394 DOI: https://doi.org/10.1016/j.rhisph.2021.100394
  33. Li, W., Zhang, Y., Tang, Z. et al. (2024). Balanced fertilization enhances the nutritional value and flavor profile of tomato fruits. Foods, 13(22), 3599. https://doi.org/10.3390/foods13223599 DOI: https://doi.org/10.3390/foods13223599
  34. Majkowska-Gadomska, J., Dobrowolski, A., Mikulewicz, E. (2016). The effect of mycorrhizal inoculum on the leaf greenness index and yield of tomato (Lycopersicon esculentum Mill.) plants grown in a heated plastic tunel. Acta Agrophys., 23(2), 445–453.
  35. Mena-Violante, H.G., Ocampo-Jimenez, O., Dendooven, L. et al. (2006). Arbuscular mycorrhizal fungi enhance fruit growth and quality of chile ancho (Capsicuman nuum L. cv San Luis) plants exposed to drought. Mycorrhiza, 16, 261–267. https://doi.org/10.1007/s00572-006-0043-z DOI: https://doi.org/10.1007/s00572-006-0043-z
  36. Morte, A., Lovisolo, C., Schubert, A. (2000). Effect of drought stress on growth and water relations of the mycorrhizal association Helianthemum almeriense – Terfezia claveryi. Mycorrhiza, 10, 115–119. https://doi.org/10.1007/s005720000066 DOI: https://doi.org/10.1007/s005720000066
  37. Natali, P.G., Piantelli, M., Sottini, A. et al. (2025). A step forward in enhancing the health-promoting properties of whole tomato as a functional food to lower the impact of non-communicable diseases. Front. Nutr., 12. https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2025.1519905 DOI: https://doi.org/10.3389/fnut.2025.1519905
  38. Patkowska, E., Konopiński, M. (2014). Antagonistic activity of selected bacteria occurring in the soil after root chicory cultivation. Plant Soil Environ., 60(7), 320–324. https://doi.org/10.17221/283/2014-PSE DOI: https://doi.org/10.17221/283/2014-PSE
  39. Pokluda, R., Ragasová, L., Jurica, M. et al. (2021). Effects of growth promoting microorganisms on tomato seedlings growing in different media conditions. PLoS One, 16(11), e0259380. https://doi.org/10.1371/journal.pone.0259380 DOI: https://doi.org/10.1371/journal.pone.0259380
  40. Pozo, M.J., Azcón-Aguilar, C. (2007). Unraveling mycorrhiza-induced resistance. Curr. Opin. Plant Biol., 10(4), 393–398. https://doi.org/10.1016/j.pbi.2007.05.004 DOI: https://doi.org/10.1016/j.pbi.2007.05.004
  41. Raiola, A., Rigano, M.M., Calafiore, R. et al. (2014). Enhancing the health-promoting effects of tomato fruit for biofortified food. Med. Inflam., 139873, 1–16. https://doi.org/10.1155/2014/139873 DOI: https://doi.org/10.1155/2014/139873
  42. Ruiz‐Lozano, J.M., Aroca, R., Zamarreño, Á.M. et al. (2016). Arbuscular mycorrhizal symbiosis induces strigolactone biosynthesis under drought and improves drought tolerance in lettuce and tomato. Plant Cell Environ., 39(2), 441–452. https://doi.org/10.1111/pce.12631 DOI: https://doi.org/10.1111/pce.12631
  43. Säle, V., Palenzuela, J., Azcón-Aguilar, C. et al. (2021). Ancient lineages of arbuscular mycorrhizal fungi provide little plant benefit. Mycorrhiza, 31(5), 559–576. DOI: https://doi.org/10.1007/s00572-021-01042-5
  44. Scervino, J.M., Ponce, M.A., Erra-Bassells, R. et al. (2007). The effect of flavones and flavonols on colonization of tomato plants by arbuscular mycorrhizal fungi of the genera Gigaspora and Glomus. Can. J. Microbiol., 53, 702–709. https://doi.org/10.1139/W07-036 DOI: https://doi.org/10.1139/W07-036
  45. Shah, K.K., Modi, B., Lamsal, B. et al. (2021). Bioactive compounds in tomato and their roles in disease prevention. Fundam. Appl. Agric., 6(2), 210–224. https://doi.org/10.5455/faa.136276 DOI: https://doi.org/10.5455/faa.136276
  46. Shenker, M., Plessner, O.E., Tel-Or, E. (2004). Manganese nutrition effects on tomato growth, chlorophyll concentration, and superoxide dismutase activity. J. Plant Physiol., 161(2), 197–202. https://doi.org/10.1078/0176-1617-00931 DOI: https://doi.org/10.1078/0176-1617-00931
  47. Singh, M., Chauhan, A., Srivastava, D.K. et al. (2024). Arbuscular mycorrhizal fungi promote growth and enhance the accumulation of bioactive compounds in tomato (Solanum lycopersicum L.). Biol. Fut., 75(2), 251–257. https://doi.org/10.1007/s42977-024-00214-6 DOI: https://doi.org/10.1007/s42977-024-00214-6
  48. Smith, S.E., Smith, F.A. (2011). Roles of arbuscular mycorrhizas in plant nutrition and growth: new paradigms from cellular to ecosystem scales. Ann. Rev. Plant Biol., 62, 227–250. https://doi.org/10.1146/annurev-arplant-042110-103846 DOI: https://doi.org/10.1146/annurev-arplant-042110-103846
  49. Song, Y., Chen, D., Lu, K. et al. (2015). Enhanced tomato disease resistance primed by arbuscular mycorrhizal fungus. Front Plant Sci., 6, 786. https://doi.org/10.3389/fpls.2015.00786 DOI: https://doi.org/10.3389/fpls.2015.00786
  50. Subramanian, K.S., Santhanakrishnan, P., Balasubramanian, P. (2006). Responses of field grown tomato plants to arbuscular mycorrhizal fungal colonization under varying intensities of drought stress. Sci. Hortic., 107(3), 245–253. https://doi.org/10.1016/j.scienta.2005.07.006 DOI: https://doi.org/10.1016/j.scienta.2005.07.006
  51. Tang, H., Hassan, M.U., Feng, L. et al. (2022) The critical role of arbuscular mycorrhizal fungi to improve drought tolerance and nitrogen use efficiency in crops. Front. Plant Sci., 13, 919166. https://doi.org/10.3389/fpls.2022.919166 DOI: https://doi.org/10.3389/fpls.2022.919166
  52. Trouvelot, A., Kough, J.L., Gianinazzi-Pearson, V. (1986). Mesure du taux de mycorhization va d’un système radiculaire recherche de methods d’estimation ayant une signification fonctionnelle. In: V., Gianinazzi-Pearson, S., Gianinazzi (eds.), Physiological and genetical aspects of mycorrhizae. INRA Publications, Paris, 217–221.
  53. Weng, W., Yan, J., Zhou, M. et al. (2022). Roles of arbuscular mycorrhizal fungi as a biocontrol agent in the control of plant diseases. Microorganisms, 10(7), 1266. https://doi.org/10.3390/microorganisms10071266 DOI: https://doi.org/10.3390/microorganisms10071266
  54. Wysocka-Owczarek M. (2010). Uprawa pomidorów w szklarniach i tunelach foliowych. Hortpress Sp. z.o. o, Warszawa
  55. Yang, Z., Li, W., Li, D. et al. (2023). Evaluation of nutritional compositions, bioactive components, and antioxidant activity of three cherry tomato varieties. Agronomy, 13(3), 637. https://doi.org/10.3390/agronomy13030637 DOI: https://doi.org/10.3390/agronomy13030637
  56. Zarea, M.J., Karimi, N., Goltapeh, E.M. et al. (2011). Effect of cropping systems and arbuscular mycorrhizal fungi on soil microbial activity and root nodule nitrogenase. Saudi Soc. Agric. Sci., 10(2),109–120. https://doi.org/10.1016/j.jssas.2011.04.003 DOI: https://doi.org/10.1016/j.jssas.2011.04.003
  57. Zhang, W., Xia, K., Feng, Z. et al. (2024). Tomato plant growth promotion and drought tolerance conferred by three arbuscular mycorrhizal fungi is mediated by lipid metabolism. Plant Physiol. Biochem., 208, 108478. https://doi.org/10.1016/j.plaphy.2024.108478 DOI: https://doi.org/10.1016/j.plaphy.2024.108478

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