Abstrakt
This study assesses the effect of meta-topolin (mT), an aromatic natural cytokinin, on micropropagation of pear rootstock OHF-333 (Pyrus сommunis L.). Cultures
were incubated in a growth chamber under controlled conditions. An in vitro culture was maintained on a modified culture medium Murashige and Skoog [1962] supplemented with meta-topolin (0 μM, 3 μM, 6 μM, 9 μM or 12 μM). After three weeks’ growth the parameters and the physiological and biochemical analysis were investigated. The results of this study suggest that the in vitro culture in the absence of cytokinins does not provide a practical solution for efficient multiplication of pear rootstocks. A good multiplication rate and high quality shoots were found at 6–9 μM mT treatment. The use of meta-topolin resulted in improvement of the leaf gas exchange and low content of phenols, as well as in the total antioxidant activity. Hence the cytokinin meta-topolin in concentrations of 6–9 μM was selected
as an optimum cytokinin level in the multiplication of pear rootstock OHF-333.
Bibliografia
Aremu, A., Bairu, M., Dolezal, K., Finnie, J., Van Staden, J. (2012). Topolins: A panacea to plant tissue culture challenges? Plant Cell Tiss Organ Cult., 108, 1–16.
Bairu, M., Stirk, W., Dolezal, K., Van Staden, J. (2007). Optimizing the micropropagation protocol for the endangered Aloe polyphylla: can meta-topolin and its derivatives serve as replacement for benzyladenine and zeatin? Plant Cell Tiss Organ Cult., 90, 15–23.
Bairu, M., Stirk, W., Dolezal, K., Van Staden, J. (2008). The role of topolins in micropropagation and somaclonal variation of banana cultivars ‘Williams’ and ‘Grand Naine’ (Musa spp. AAA). Plant Cell Tiss Organ Cult., 95, 373–379.
Baroja-Fernandez, E., Aguirreolea, J., Martienkova, H., Hanus, J., Strnad, M. (2002). Aromatic cytokinins in micropropagated potato plants. Plant Physiol. Biochem., 40, 217–224.
Beadle, C. (1993). Growth analysis. Photosynthesis and production in a changing environment. Springer-Sci.+Buisness Media, B.V., 36–46.
Bell, R., Reed, B. (2002). In vitro tissue culture of pear: advances in techniques for micropropagation and germplasm preservation. Acta Hort., 596, 412–418.
Bogaert, I., Van Cauter, S., Werbrouck, S., Doležal, K. (2006). New aromatic cytokinins can make the difference. Acta Hortic., 725, 265–270.
Caers, M., Vendrig, C. (1986). Benzyladenine effects on the development of the photosynthetic apparatus in Zea mays: Studies on photosynthetic activity, enzymes and (etio) chloroplast ultrastructure. Physiol. Plant., 66, 685–691.
Fletcher, A., Kallidumbil, V., Steele, P. (1982). Stimulation of chlorophyll synthesis in cucumber using cucumber cotyledons. Plant Physiol., 69, 937–939.
Fujiwara, K., Kira, S., Kozai, T. (1992). Time course of CO2 exchange of potato cultures in vitro with different sucrose concentration in the culture medium. J. Agr. Met., 48, 49–56.
Harrar, Y., Bellec, Y., Bellini, C., Faure, J-D. (2003). Hormonal control of cell proliferation requires PASTICCINO genes. Plant Physiol., 132, 1217–1227.
Howell, S., H., Lall, S., Che, P. (2003). Cytokinins and shoot development. Trends Plant Sci., 8, 453–459.
Huetteman, C., Preece, J. (1993). Thidiazuron – a potent cytokinin for woody plant tissue culture. Plant Cell Tiss. Org., 33, 105–119.
Jones, L., Martinkovaґ, H., Strnad, M., Hanke, D. (1996). Occurrence of aromatic cytokinins in oil palm (Elaeis guineensis Jacq.). J. Plant Growth Regul., 15, 39–49.
Kaminek, M., Vanek, T., Kalendova, A., Pilar, J. (1987). Effect of two cytokinins on production of stem cuttings by stock plants of Euphorbia pulcherrima Wild. and Gerbera jamesonii Hook. Sci. Hortic., 33, 281–289.
Kubalakova, M., Strnad, M. (1992). The effect of aromatic cytokinins (populins) on micropropagation and regeneration in vitro. Biol. Plant., 34, 578–579.
Lambert, P., Westwood, M. (1987). Pear rootstocks. In: Rootstocks for fruit crops, Rom, R., Carlson, R. (eds). Willey, New York.
Leshem, B., Shaley, D., Izhar, S. (1988). Cytokinin as an inducer of hyperhydricity in melon. Ann. Bot., 61, 255–260.
Lichtenthaler, H., Wellburn, A. (1983). Determination of total carotenoids and chlorophyll a and b of leaf extracts in different solvents. Biochem. Soc. Trans., 603, 591–592.
Magyar-Tabori, K., Dobranszki, J., Teixeira da Silva, J., Bulley, S., Hudak, I. (2010). The role of cytokinins in shoot organogenesis in apple. Plant Cell Tiss Organ Cult., 101, 251–267.
Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bio assays for tobacco tissue cultures. Physiol. Plant., 15, 473–497.
Parthier, B. (1979). Phytohormones and chloroplast development. Biochem. Physiol. Pflanzen., 174, 173–214.
Podwyszyńska, M., Węgrzynowicz-Lesiak, E., Dolezal, K., Krekule, K., Strnad, M., Saniewski, M. (2012). New cytokinin – meta-methoxytopolins in micropropagation of Cotinus coggygria Scop. ‘Royal Purple’. Prop. Ornam. Plants., 12, 4, 220–228.
Pospisilova, J., Catsky, J., Sestak, Z. (1996). Photosynthesis in plants cultured in vitro. In: Handbook of photosynthesis, Pessarali, M. (ed.). Marcel Dekker, NY. Roels, S., Escalona, M., Cejas, I., Noceda, C., Rodriguez, R., Canal, M.J., Sandoval, J., Debergh, P. (2005). Optimization of plantain (Musa AAB) micropropagation by temporary immersion system. Plant Cell Tiss Organ Cult., 82, 57–66.
Rossi, M., Giussani, E., Morelli, R., Lo Scalzo, R., Nani, C., Torreggiani, D. (2003). Effect of fruit blanching on phenolics and radical scavenging activity of highbush blueberry juice. Food Res. Intern., 36, 999–1005.
Ruzic, D., Vujovic, T., Nikolic, D., Cerovic, R. (2011). In vitro growth responses of the ‘Pyrodwarf’ pear rootstock to cytokinin types. Romanian Biotechnol. Lett., 16, 5, 6630–6637.
Salvi, N., George, L., Eapen, S. (2002). Micropropagation and field evaluation of micropropagated plants of turmeric. Plant Cell Tissue Organ Cult., 68, 143–151.
Sengul, M., Yildiz, H., Gungor, N., Cetin, B., Eser, Z., Ercisli, S. (2009). Total phenolic content, antioxidant and antimicrobial activities of some medicinal plants. J. Pharm Sci., 22, 1, 102–6.
Singleton, V., Rossi, J. (1965). Colorunetry of total phenolics with phosphomolybdicphosphotungstic acid reagents. Amer. J. Enol. Viticult., 16, 144–58.
Smulders, M., De Klerk, G. (2011). Epigenetics in plant tissue culture. Plant Growth Regul., 63, 137–146.
Strnad, M., Hanus, J., Vanek, T., Kaminek, M., Ballantine, J., Fussell, B., Hanke, D. (1997). Metatopolin, a highly active aromatic cytokinin from poplar leaves (Populus canadensis Moench, cv. Robusta). Phytochemistry, 45, 213–218.
Tarkowska, D., Dolezal, K., Tarkowski, P., Astot, C., Holub, J., Fuksova, K., Schmulling, T., Sandberg, G., Strnad, M. (2003). Identification of new aromatic cytokinins in Arabidopsis thaliana and Populus canadensis leaves by LC-(+)ESIMS and capillary liquid chromatography frit-fast atom bombardment mass spectrometry. Physiol. Plant., 117(4), 579–590.
Teramoto, H., Momotani, E., Tsuji, H. (1993). Benzyladenine-induced changes in the translatable mRNA population in excised cucumber cotyledons. Physiol. Plant., 87, 584–591.
Van der Zwet, T., Beer, S. (1995). Fire blight – Its Nature, Prevention and Control. U.S. Department of Agriculture, Agricultural Information Bulletin No. 631.
Werbrouck, S. (2010). Merits and drawbacks of new aromatic cytokinins in plant tissue culture. Acta Hortic., 865,103–108.
Werbrouck, S., Strnad, M., Van Onckelen, H., Debergh, P. (1996). Meta-topolin, an alternative to benzyladenine in tissue culture? Physiol. Plant., 98, 291–297.
Wertheim, S. (2002). Rootstocks for European pear: a Review. Acta Hortic., 596, 299–309.
Wojtania, A., Gabryszewska, E. (2001). Effect of cytokinins and amino acids on multiplication of Pelargonium cultivars. Acta Soc. Bot. Pol., 70, 203–207.
Yen, G., Chen, H. (1995). Antioxidant activity of various tea extracts in relation to their antimutagenicity. J. Agric. Food Chem., 43, 1, 27–32.
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