Przejdź do głównego menu Przejdź do sekcji głównej Przejdź do stopki
ASPHC Baner

Tom 19 Nr 5 (2020)

Artykuły

Micropropagation of some Onobrychis species through in vitro shoot regeneration

DOI: https://doi.org/10.24326/asphc.2020.5.5
Przesłane: 7 maja 2019
Opublikowane: 2020-10-29

Abstrakt

Onobrychis species have an extensive spread in Turkey and adapted to various environmental conditions. In this study, it was aimed to propagate with tissue culture methods of endemic Onobrychis fallax Freyn & Sint. ex Freyn var. longifolia Aktoklu, Onobrychis stenostachya subsp. sosnowskyi, Onobrychis elata Boiss. & Balansa and worldwide commonly cultivated Onobrychis viciifolia Scop. species. For this purpose, cotyledon node explants were cultured in Murashige and Skoog (MS) media containing various concentration of 6-benzylaminopurine (BAP), thidiazuron (TDZ) or meta-Topolin (mT) alone or in combination with 0.5 mg L–1 NAA. Significant variations in number of shoots per explant was observed depending on the species and growth regulators. The greatest number of shoots per explant was obtained from 1.2 mg L–1 TDZ treatments in O. stenostachya, O. fallax and O. elata and from 0.3 mg L–1 TDZ treatments in O. viciifolia, whereby 10.58, 9.50, 5.5, 6.42 shoots were recorded. Resultant shoots were rooted in half strength MS nutrient media containing 1 or 2 mg L–1 indole-3-butyric acid (IBA). The rooting ratio was 86.11% in O. viciifolia, 50.00% in O. stenostachya and 36.11% in O. fallax and O. elata.

Bibliografia

  1. Aktoklu, E. (2001). Two new varieties and a new record in Onobrychis from Turkey. Turk. J. Bot., 25, 359–363.
  2. Ambarlı, D., Zeydanlı, U.S., Balkız, Ö., Aslan, S., Karaçetin, E., Sözen, M., Ilgaz, Ç., Gürsoy Ergen, A., Lise, Y., Demirbaş Çağlayan, S., Welch, H.J., Welch, G., Turak, A.S., Can Bilgin, C., Özkil, A., Welch, H.J. (2016). An overview of biodiversity and conservation status of steppes of the Anatolian Biogeographical Region. Biodivers. Conserv., 25(12), 2491–2519. DOI: 10.1007/s10531-016-1172-0
  3. Avcı, S., Kaya, M.D. (2013). Seed and germination characteristics of wild Onobrychis taxa in Turkey. Turk. J. Agric. For., 37(5), 555–560. DOI: 10.3906/tar-1903-40
  4. Avcı, S., Ilhan, E., Erayman, M., Sancak, C. (2014). Analysis of Onobrychıs Genetic Diversity Using SSR Markers from Related Legume Species. J. Anim. Plant Sci., 24(2), 556–566.
  5. Avcı, S., Tekin, N., Sancak, C., Özcan, S., Marangi, A.O. (2016). Phylogenetic relationship of some Onobrychis taxa naturally grown in Turkey based on morphology and nuclear ribosomal DNA ITS Sequences. Legume Res., 39(5), 665–673. DOI: 10.18805/lr.v0iOF.11184
  6. Babaoğlu, M., Gürel, E., Özcan, S. (2001). Bitki Biyoteknolojisi I. Doku Kültürü ve Uygulamaları, Selçuk Üniversitesi Basımevi, 71–88 [in Turkish].
  7. Cézar, T.M., Higa, A.R., Koehler, H.S., Ribas, L.L.F. (2015). Influence of culture medium, explant length and genotype on micropropagation of Pinus taeda L. Ciênc. Florest., 25(1), 13–22. DOI: 10.1590/1980-509820152505013
  8. Catterou, M., Dubois, F., Smets, R., Vaniet, S., Kichey, T., Van Onckelen, H., Sangwan-Norreel, B.S, Sangwan, R.S. (2002). hoc: an Arabidopsis mutant overproducing cytokinins and expressing high in vitro organogenic capacity. Plant J., 30(3), 273–287. DOI: 10.1046/j.1365-313X.2002.01286.x
  9. Çeliktaş, N., Can, E., Hatipoğlu, R., Avcı, S. (2006). Somatic embryogenesis, callus production, and plantlet growth in sainfoin (Onobrychis viciifolia Scop.). New Zealand J. Agric. Res., 49(4), 383–388. DOI: 10.1080/00288233.2006.9513728
  10. Çöçü, S., Uranbey, S., Sancak, C. (2003). Bazı yaygın fiğ (Vicia sativa L.) Çeşitlerinde Olgunlaşmamış Embriyo Eksplantlarından Adventif Sürgün Rejenerasyonu. Ankara Üniversitesi Ziraat Fak. Tarım Bilimleri Dergisi, 9(4), 445–449.
  11. Demirkuş, N., Koyuncu, M., Gul, M. (2018). Endemic Plants of Lake Van Basin. Anatol. J. Bot., 2(2), 70–83. DOI: 10.30616/ajb.401062
  12. Dimitrova, N., Nacheva, L., Berova, M. (2016). Effect of meta-topolin on the shoot multiplication of pear rootstock OHF-333 (Pyrus communis L.). Acta Sci. Pol. Hortorum Cultus, 15, 43–53.
  13. Erkovan, H.I., Gullap, M.K., Erkovan, S., Koç, A. (2016). Horned sainfoin (Onobrychis Cornuta (L.) Desv.): is it an amusing or nuisance plant for steppe rangelands. Ecol. Saf., 10, 1314–7234.
  14. Erişen, S. (2005). Yonca (Medicago sativa L.)’da somatik embriyogenesis aracılığıyla bitki rejenerasyonu. Tarım Bilimleri Dergisi, 11(3), 311–315.
  15. Erişen, S., Atalay, E., Yorgancilar, M. (2011). The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey. Turk. J. Bot., 35(5), 521–526. DOI: 10.3906/bot-1009-74
  16. Gentile, A., Gutiérrez, M.J., Martinez, J., Frattarelli, A., Nota, P., Caboni, E. (2014). Effect of meta-Topolin on micropropagation and adventitious shoot regeneration in Prunus rootstocks. Plant Cell, Tissue Organ Cult., 118(3), 373–381. DOI: 10.1007/s11240-014-0489-1
  17. Gomes, F., Simoes, M., Lopes, M.L., Canhoto, J.M. (2010). Effect of plant growth regulators and genotype on the micropropagation of adult trees of Arbutus unedo L. (strawberry tree). New Biotechnol., 27(6), 882–892. DOI: 10.1016/j.nbt.2010.02.009
  18. Guo, B., Abbasi, B.H., Zeb, A., Xu, L., Wei, Y.H. (2011). Thidiazuron: A multi-dimensional plant growth regulator. Afric. J. Biotechnol., 10(45), 8984–9000. DOI: 10.5897/AJB11.636
  19. Hayot Carbonero, C., Mueller-Harvey, I., Brown, T.A., Smith, L. (2011). Sainfoin (Onobrychis viciifolia): a beneficial forage legume. Plant Genet. Resour., 9, 70–85. DOI: 10.1017/S1479262110000328
  20. Hill, K., Schaller, G.E. (2013). Enhancing plant regeneration in tissue culture: a molecular approach through manipulation of cytokinin sensitivity. Plant Signal. Behav., 8(10), 212–224. DOI: 10.4161/psb.25709
  21. Honarmand, L., Zare, N., Asghari, R., Sheikhzadeh, P. (2016). In vitro regeneration of sainfoin (Onobrychis sativa) via shoot apex explant. Iran. J. Field Crop Sci., 47(2), 315–328 [in Persian].
  22. Karakuş, Ş. (2016). Flora of Malatya. İnönü Üniversity Graduate of Natural Applied Sciences PhD Thesis, Turkey [in Turkish].
  23. Magyar-Tábori, K., Dobránszki, J., Silva, J.A.T. da, Bulley, S.M., Hudák, I. (2010). The role of cytokinins in shoot organogenesis in apple. Plant Cell, Tissue Organ Cult., 101(3), 251–267. DOI: 10.1007/s11240-010-9696-6
  24. Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant., 15(3), 473–497.
  25. Özcan, S., Yıldız, M., Sancak, C., Özgen, M. (1996a). Adventitious shoot regeneration in sainfoin (Onobrychis viciifolia Scop.). Turk. J. Bot., 20, 497–501.
  26. Özcan, S., Sevimay, C.S., Yıldız, M., Sancak, C., Özgen, M. (1996b). Prolific shoot regeneration from immature embriyo explants of sainfoin (Onobrychis viciifolia Scop.). Plant Cell Rep., 16, 200–203.
  27. Özgen, M., Özcan, S., Sevimay, C.S., Sancak, C., Yıldız, M. (1998). High frequency adventitious shoot regeneration in sainfoin. Plant Cell, Tissue Organ Cult., 52, 205–208. DOI: 10.1023/A:1006097714968
  28. Sancak, C., 1999. In vitro micropropagation of sainfoin (Onobrychis viciifolia Scop.). Turk. J. Bot., 23, 133–136.
  29. Strnad, M., Hanus, J., Vanek, T., Kaminek, M., Ballantine, J., Fussell, B., Hanke, D. (1997) Meta-Topolin, a highly active aromatic cytokinin from poplar leaves (Populus canadensis Moench, cv. Robusta). Phytochemistry, 45, 213–218.
  30. Uzun, S. (2012). Korunganın (Onobrychis viciifolia Scop.) hipokotil ve kotiledon eksplantlarından adventif sürgün rejenerastonu. Türk Doğa ve Fen Dergisi, 1, 126–130 [in Turkish].
  31. Vijayakumar, J., Ponmanickam, P., Samuel, P., Sudarmani, D.N.P., Pandiarajan, J. (2017). Influence of meta-topolin on efficient plant regeneration via micropropagation and organogenesis of safflower (Carthamus tinctorius L.) cv. NARI-H-15. Amer. J. Plant Sci., 8(04), 688. DOI: 10.4236/ajps.2017.84048
  32. Yorgancilar, M., Erisen, S. (2011). The effect of thidiazuron (TDZ) on shoot regeneration of Astragalus schizopterus. J. Anim. Plant Sci., 21(3), 519–524.

Downloads

Download data is not yet available.

Podobne artykuły

1 2 3 4 > >> 

Możesz również Rozpocznij zaawansowane wyszukiwanie podobieństw dla tego artykułu.