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ASPHC Baner

Vol. 18 No. 4 (2019)

Articles

In vitro proliferation and ex vitro rooting of microshoots of lisianthus using auxin and cytokinin on the solid, liquid and double-phase culture systems

DOI: https://doi.org/10.24326/asphc.2019.4.5
Submitted: August 5, 2019
Published: 2019-08-07

Abstract

A protocol was developed for high frequency and low cost of in vitro shoot proliferation and ex vitro rooting of Eustoma grandiflorum (Gentianaceae) on solid medium. Shoot tips as explants were cultured on Murashige and Skoog (MS) medium enriched with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0.00, 0.01, 0.10 and 1.00 mg l–1) and 6-benzylaminopurine (BAP) (0.00, 0.50, 2.00 and
5.00 mg l–1). Three culture media systems (solid, liquid and double-phase) were applied. None of the explants cultured on liquid and double-phase media resulted in live plant production. Maximum axillary shoot number (54.45) was recorded in the plantlets treated with 0.10 mg l–1 2,4-D in combination with 5.00 mg l–1 BAP. Treatment of 0.01 mg l–1 2,4-D along with 0.50 mg l–1 BAP produced maximum node number and internode length. Some shoots produced on medium containing plant growth regulators (PGRs) were rooted in soil. The largest number (5.50/plantlet) and longest length of root (7.75 cm/plantlet) were obtained in ex vitro condition on the base of shoots produced in culture medium enriched with 0.10 mg l–1 2,4-D along with
0.50 mg l–1 BAP. The combination of 1.00 mg l–1 2,4-D and 0.50 mg l–1 BAP was found to be the most suitable PGRs for obtaining the highest callus weight. The most fresh weight was calculated from plantlets grown on the medium containing 0.10 mg l–1 2,4-D along with 5.00 mg l–1 BAP. Maximum dry weight was obtained in free-PGRs medium. About 90% of the rooted plantlets were established successfully in cultivation beds. Acclimatized plants were morphologically similar to the mother plants.

References

  1. Amitage, A.M., Laushmam, J.M. (1993). Specialty cut flowers: the production of annuals, perennials, bulbs, and woody plants for fresh and dried cut flowers. Timber Press, 279–289.
  2. Chawla, H.S. (2009). Introduction to plant biotechnology. Springer.
  3. Costa, F.H.S., Pereira, M.A.A., Oliveira, J.P., Scherwinski-Pereira, J.E. (2007). Efeito deagentes geleificantes alternativos no meio de cultura no cultivo in vitro de aba-caxizeiro e bananeira. Ciênc. Agrot., 31, 41–46.
  4. De Oliveira, S.O.D., Sayd, R.M., Balzon, T.A., Scherwinski-Pereira, J.E. (2013). A new procedure for in vitro propagation of vanilla (Vanilla planifolia) using a double-phase culture system. Sci. Hortic., 161, 204–209.
  5. Furukawa, H., Matsubara, C., Shigematsu, N. (1990). Shoot regeneration from the roots of Prairie gentian [Eustoma grandiflorum (Griseb.) Schinners]. Plant Tiss. Cult. Lett., 7(1), 11–13.
  6. Ghafari Esizad, S., Kaviani, B., Tarang, A.R., Bohlooli Zanjani, S. (2012). Micropropagation of lisianthus, an ornamental plant. Plant Omics J., 5, 314–319.
  7. Gomes, F., Simões, M., Lopes, M.L., Canhoto, M. (2010). Effect of plant growth regulators and genotype on the micropropagation of adult trees of Arbutus unedo L. (strawberry tree). New Biotech., 45(1), 72–82.
  8. Han, B.H., Yu, H.J., Yae, B.W., Peak, K.Y. (2004). In vitro micropropagation of Lilium longiflorum ‘Georgia’ by shoot formation as influenced by addition of liquid medium. Sci. Hortic., 103, 39–49.
  9. Harbaugh, B.K. (2006). Lisianthus, Eustoma grandiflorum. In: Flower breeding and genetics, Anderson, N.O. (ed.). Springer, Netherlands, 645–663.
  10. Hashemabadi, D., Kaviani, B. (2010). In vitro proliferation of an important medicinal plant Aloe-A method for rapid production. Aus. J. Crop Sci., 4(4), 216–222.
  11. Jahan, M.T., Islam, M.R., Khan, R., Mamun, A.N.K., Ahmed, G., Hakim, H. (2009). In vitro clonal propagation of Anthurium (Anthurium andraeanum Lind)
  12. using callus culture. Plant Tiss. Cult. Biotech., 19(1), 61–69.
  13. Jain, S.M., Ochatt, S.J. (2010). Protocols for in vitro propagation of ornamental plants. Springer protocols, Humana Press.
  14. Kaviani, B., Ahmadi Hesar, A., Tarang, A.R., Bohlooli Zanjani, S., Hashemabadi, D., Rezaei, M.A. (2011). Callus induction and root formation on the leaf micro-cuttings of Matthiola incana using Kn and NAA. Am.-Eurasian J. Agric. Environ. Sci., 11(3), 456–461.
  15. Kaviani, B., Zamiraei, F., Bohlooli Zanjani, S., Tarang, A.R., Mohammadi Torkashvand, A. (2014). In vitro flowering and micropropagation of Lisianthus (Eustoma grandiflorum) in response to plant growth regulators (NAA
  16. and BA). Acta Sci. Pol. Hortorum Cultus, 13(4), 145–155.
  17. Khorrami Raad, M., Bohlooli Zanjani, S., Shoor, M., Hamidoghli, Y., Ramezani Sayyad, A., Kharabian-Masouleh, A., Kaviani, B. (2012). Callus induction and organogenesis capacity from lamina and petiole explants of Anthurium andreanum Linden (Casino and Antadra). Aus. J. Crop Sci., 6(5), 928–937.
  18. Kunitake, H., Nakashima, T., Mori, K., Tanaka, M., Mii, M. (1995). Plant regeneration from mesophyll protoplasts of lisianthus (Eustoma grandiflorum) by adding activated charcoal into protoplast culture medium. Plant Cell Tiss. Org. Cult., 43, 59–65.
  19. Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant, 15, 473–497.
  20. Nhut, D.T., Hai, N.T., Phan, M.X. (2010). A highly efficient protocol for micropropagation of Begonia tuberous. In: Protocols for in vitro propagation of ornamental plants, Jain, S.M., Ochatt, S.J. (eds.). Springer Protocols, Humana Press, 15–20 pp.
  21. Ordogh, M.‚ Jambor-Benczur, E., Tilly-Mandy, A. (2006). Micropropagation of ‘Echo’ cultivars of Eustoma grandiflorum. (ISHS) Acta Hortic., 725, 457–460.
  22. Paek, K.Y., Hahn, E.J. (2000). Cytokinins, auxins and activated charcoal affect organogenesis and anatomical characteristics of shoot-tip cultures of lisianthus (Eustoma grandiflorum (Raf.) Shinn). In Vitro Cell Dev. Biol. Plant, 36, 128–132.
  23. Pati, P.K., Rath, S.P., Sharma, M., Sood, A., Ahuja, P.S. (2005). In vitro propagation of rose-a review. Biotech. Adv., 24(1), 94–114.
  24. Pierik, R.L.M. (1987). In vitro culture of higher plants. Nijhoff, Dordrecht, 45–82.
  25. Pullman, G.S., Skryabina, A. (2007). Liquid medium and liquid overlays improve embryogenic tissue initiation in conifers. Plant Cell Rep., 26, 873–887.
  26. Reddy, J.H., Bopaiah, A.K., Abhilash, M. (2011). In vitro micropropagation of Anthurium digitatum, using leaf as explants. Asian J. Pharma. Health Sci., 1(2), 70–74.
  27. Scherwinski-Pereira, J.E., Lima, E.C.A., Silva, T.L.S., Mesquita, A.G.G., Maciel, S.A., Costa, F.H.S. (2012). Double-phase culture system for large scale production of pineapple. Plant Cell Tiss. Org. Cult., 109,
  28. –269.
  29. Van Staden, D., Zazimalora, E., Georgw, E.F. (2008). Plant growth regulators, II: cytokinins, their analogues and inhibitors. In: Plant propagation by tissue culture, George, E.F. et al. (eds.). Ed. 3. Springer, Dordecht, 205–226.
  30. Viĕgas, J., Da Rocha, M.T.R., Feffeira-Moura, I., Da Rosa, D.L., De Souza, J.A., Corrȇa, M.G.S., Da Silva, J.A.T. (2007). Anthurium andreanum (Linden ex André) culture: in vitro and ex vitro. Floricult. Ornam. Biotech., 1(1), 61–65.

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