Abstract
Present study aimed to reveal the effects of jasmonic acid and activated charcoal on in vitro carrot plantlet regeneration by using Murashige and Skoog (MS) medium supplemented with BAP, NAA, activated charcoal and jasmonic acid at various concentrations. To serve the purpose, in vitro carrot seed germination, shoots, cotyledons, and first leaves formation of orange and purple carrot plantlets were investigated. During the experiments, root size, weight, and size of petiole diameter, hyperhydricity and callus formation rate were recorded. Experimental results revealed that combination of jasmonic acid and activated charcoal in medium had a positive effect especially on the first stage of developmental processes such as seed swelling and germination, cotyledon and first leaf formation as well as having positive effects on above-ground internode elongation, petiole and plantlet height.
References
- Ahmadi, B., Shariatpanahi, M.E., da Silva, J.A.T. (2014). Efficient induction of microspore embryogenesis using abscisic acid, jasmonic acid and salicylic acid in Brassica napus L. Plant Cell Tiss. Organ Cult., 116, 343–351. DOI: 10.1007/s11240-013-0408-x
- Baktır, İ. (2015). Jasmonateler (Yasemin Asitleri). Hormonlar, Bitki Gelişim Düzenleyicileri, Özellikleri ve Tarımda Kullanımları [Jasmonates (Jasmine Acids). Hormones, Plant Growth Regulators, Properties and Uses in Agriculture]. İstanbul, Türkiye: Hasad Yayıncılık, sf. 90–92 [in Turkish].
- Berger, S., Bell, E., Mullet, J.E. (1996). Two methyl jasmonate-insensitive mutants show altered expression of AtVsp in response to methyl jasmonate and wounding. Plant Physiol., 111, 525–531. DOI: 10.1104/pp.111.2.525
- Creelman, R.A., Mullet, J.E. (1997). Biosynthesis and action jasmonates in plants. Ann. Rev. Plant Physiol. Plant Mol. Biol., 48, 355–381.
- Çavusoglu, K., Kabar, K. (2006). Does Jasmonic Acid Prevent The Germination of Barley Seeds? SDÜ Fen Edebiyat Fakültesi Fen Dergisi (E-Dergi), 1(12–2), 35–41.
- Çavusoglu, K., Kabar, K., Kılıç, S. (2007). Effects of Some Plant Growth Regulators on Jasmonic Acid Induced Inhibition of Seed Germination and seedling Growth of Barley. SDÜ Fen Edebiyat Fakültesi. Fen Dergisi (E-Dergi), 2(1), 53–59.
- Dereli, U. (2010). Siyah Havuç Suyu Konsantresi Üretimi ve Depolanması Sürecinde Fenolik Maddelerdeki Değişimler ve Bu Değişimlerin Antioksidan Aktivite ile İlişkisi. Yüksek Lisans Tezi. Ankara Üniversitesi, Fen Bilimleri Enstitüsü.
- Gaspar, T., Kevers, C., Penel, C., Greppin, H., Reid, D.M., Thorpe, T.A. (1996). Plant Hormones and Plant Growth Regulators In Plant Tissue Culture. In vitro Cell. Dev. Biol.-Plant, 32, 272–289.
- Grebenstein, C., Kos, S.P., de Jong, T.J., Tamis, W.L.M., de Snoo, G.R. (2013). Morphological markers for the detection of introgression from cultivated into wild carrot (Daucus carota L.) reveal dominant domestication traits. Plant Biol., 15, 531–540.
- Hanci, F., Cebeci, E. (2012). Siyah Havuç (Daucus carota ssp. sativus var. atrorubens Alef.) Yetiştiriciliği. Tarım Türk Dergisi, 7, 36–40 [in Turkish].
- Jourdan, M., Gagné, S., Dubois-Laurent, C., Maghraoui, M., Huet, S., Suel, A., Hamama, L., Briard, M., Peltier, D., Geoffriau, E. (2015). Carotenoid content and root color of cultivated carrot: a candidate-gene association study using an original broad unstructured population. PLoS One, 10:e0116674, 1–19. DOI: 10.1371/journal.pone.0116674
- Kenar, S. (2013). Patateste (Solanum tuberosum L.) in vitro Mikrotuberizasyon Üzerine Jasmonik Asit – Giberellik Asit Etkileşiminin Araştırılması. Yüksek Lisans Tezi, Hacettepe Üniversitesi, Ankara, Turkey [in Turkish].
- Kepczynski, J., Bialecka, B., Kepczynska, E. (1999). Ethylene biosynthesis in Amaranthus caudatus seeds in response to methyl jasmonate. J. Plant Growth Regul., 28, 59–65. DOI: 10.1023/A:1006201625637
- Koda, Y., Kikuta, Y., Takazi, H., Tsujino, Y., Sakamura, S., Yoshihara, T. (1991). Potato tuber-inducing activities of jasmonic acid and related compounds. Phytochemistry, 30(5), 1435–1438. DOI: 10.1016/0031-9422(91)84180-Z
- Koda, Y., Kikuta, Y. (1991). Possible involvement of jasmonic acid in tuberization in yam plants. Plant Cell Physiol., 32, 629–633.
- Koda, Y., Kikuta, Y. (2001). Effects of jasmonates on in vitro tuberization in several potato cultivars that differ greatly in maturity. Plant Prod. Sci., 4(1), 66–70. DOI: 10.1626/pps.4.66
- Lorenzo, O., Solano, R. (2005). Molecular players regulating the jasmonate signalling network. Curr. Opin. Plant Biol., 8, 532–540. DOI: 10.1016/j.pbi.2005.07.003.
- Luby, C.H., Maeda, H.A., Goldman, I.L. (2014). Genetic and phenological variation of tocochromanol (vitamin E) content in wild (Daucus carota L. var. carota) and domesticated carrot (D. carota L. var. sativa). Hortic. Res., 1(15), 1–6. DOI: 10.1038/hortres.2014
- Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15, 473–97.
- Nowacka, M., Wedzik, M. (2016). Effect of ultrasound treatment onmicrostructure, colour and carotenoid content in fresh and dried carrot tissue. Appl. Acoust., 103, 163–171. DOI: 10.1016/j.apacoust.2015.06.011
- Pelacho, A.M., Mingo-Castel, A.M. (1991). Jasmonic acid induces tuberization of potato stolons cultured in vitro. Plant Physiol., 97, 1253–1255. DOI: 10.1104/pp.97.3.1253
- Parthier, B. (1991). Jasmonates, new regulators of plant growth and development: Many facts and few hypotheses of their action. Bot. Acta, 104, 446–454. DOI: 10.1111/j.1438-8677.1991.tb00257.x
- Rathinapriya, P., Satish, L., Rameshkumar, R., Pandian, S., Rency, A.S., Ramesh, M. (2019). Role of activated charcoal and amino acids in developing an efficient regeneration system for foxtail millet (Setaria italica (L.) Beauv.) using leaf base segments. Physiol. Mol. Biol. Plants, 25(2), 533–548. DOI: 10.1007/s12298-018-0619-z
- Ravnikar, M., Gogala, N. (1990). Regulation of potato meristem development by jasmonic acid in vitro. J. Plant Growth Regul., 9, 233–236. DOI: 10.1007/BF02041968
- Ravnikar, M., Vilhar, B., Gogala, N. (1992). Stimulatory effects of jasmonic acid on potato node and protoplast culture. J. Plant Growth Regul., 11(1), 29–33. DOI: 10.1007/BF00193840
- Ravnikar, M., Žel, J., Plaper, I., Špacapan, A. (1993). Jasmonic acid stimulates shoot and bulb formation of garlic in vitro. J. Plant Growth Reg., 12(2), 73–77. DOI: 10.1007/BF00193236
- Sarkar, D., Pandey, S.K., Sharma, S. (2006). Cytokinins antagonize the jasmonates action on the regulation of potato (Solanum tuberosum) tuber formation in vitro. Plant Cell, Tissue Organ Cult., 87, 285–295. DOI: 10.1007/s11240-006-9166-3
- Teixeira da Silva, J.A. (2012). Jasmonic Acid, but not Salicylic Acid, Improves PLB Formation of Hybrid Cymbidium. Plant Tissue Cult. Biotech., 22(2), 187–192. DOI: 10.3329/ptcb.v22i2.14209
- Thomas, D. (2008). The role of activated charcoal in plant tissue culture. Biotechnol. Adv., 26, 618–631. DOI: 10.1016/j.biotechadv.2008.08.003
- Tsai, S.J., Juengel, J.L., Wiltbank, M.C. (1997). Hormonal regulation of monocyte chemoattractant protein-1 messenger ribonucleic acid expression in corpora lutea. Endocrinology, 138, 4517–4520. DOI: 10.1210/endo.138.10.5577
- Wang, G.L., Que, F., Xu, Z.S., Wang, F., Xiong, A.S. (2017). Exogenous gibberellin enhances secondary xylem development and lignification in carrot taproot. Protoplasma, 254, 839–848. DOI: 10.1007/s00709-016-0995-6
- Yazawa, K., Takahata, K., Kamada, H. (2004). Isolation of the gene encoding carrot leafy cotyledon1 and expression analysis during somatic and zygotic embryogenesis. Plant Physiol. Biochem., 42(3), 215–223. DOI: 10.1016/j.plaphy.2003.12.003
- Wilen, R.W., Mandel, R.M., Pharis, R.P., Holbrook, L.A., Moloney, M.M. (1990). Effects of abscisic acid and high osmoticum on storage protein gene expression in microspore embryos of Brassica napus. Plant Physiol., 94, 875–881. DOI: 10.1104/pp.94.3.875
Downloads
Download data is not yet available.
-
Tour Jan,
Ikram Ullah,
Bilal Muhammad,
_ Tariq,
Ali Mansoor,
Zaheer Ullah,
Muhammad Asif Nawaz,
EFFICIENT in vitro PROPAGATION OF Amaranthus viridis L. USING NODE EXPLANTS
,
Acta Scientiarum Polonorum Hortorum Cultus: Vol. 19 No. 4 (2020)
-
Weronika Kursa,
Agmieszka Jamiołkowska,
Barbara Skwaryło-Bednarz,
Radosław Kowalski,
Jakub Wyrostek,
Elżbieta Patkowska,
Marek Kopacki,
In vitro efficacy of herbal plant extracts on some phytopathogenic fungi
,
Acta Scientiarum Polonorum Hortorum Cultus: Vol. 21 No. 6 (2022)
-
Elżbieta Paduch-Cichal,
Tomasz Krupa,
Ewa Mirzwa-Mróz,
Marek Stefan Szyndel,
Karol Staniszewski,
Wojciech Kukuła,
Elżbieta Mielniczuk,
Marcin Wit,
Wojciech Wakuliński,
Effect of virus infection on the fruit quality of sour cherry cultivar Łutówka
,
Acta Scientiarum Polonorum Hortorum Cultus: Vol. 23 No. 2 (2024)
-
Zahra Karimian,
Leila Samiei,
Jafar Nabati,
ALLEVIATING THE SALT STRESS EFFECTS IN Salvia splendens BY HUMIC ACID APPLICATION
,
Acta Scientiarum Polonorum Hortorum Cultus: Vol. 18 No. 5 (2019)
-
Muhammad T. Tahir,
Muhammad A. Anjum,
Muhammad Saqib,
Muhammad F. Khalid,
Sajjad Hussain,
Seed primig and foliar application of plant growth regulators affect the growth and yield of okra under calcareous soils
,
Acta Scientiarum Polonorum Hortorum Cultus: Vol. 18 No. 4 (2019)
-
Magdalena Dyduch-Siemińska,
Wiktoria Błaszczyk,
Jacek Gawroński,
Influence of medium type and culture method on the multiplication efficiency of Chlorophytum comosum (Thunb.) Jacques in in vitro conditions
,
Acta Scientiarum Polonorum Hortorum Cultus: Vol. 22 No. 6 (2023)
-
Ratibor Štrbanović,
Dobrivoj Poštić,
Marijenka Tabaković,
Jasmina Knežević,
Ljubiša Živanović,
Rade Stanisavljević,
Effects of pre-sowing seed treatments for improving germination and the growth of pepper and tomato seedlings
,
Acta Scientiarum Polonorum Hortorum Cultus: Vol. 20 No. 1 (2021)
-
Haluk Çağlar Kaymak,
Adem Aksoy,
Recep Kotan,
INOCULATION WITH N2-FIXING PLANT GROWTH PROMOTING RHIZOBACTERIA TO REDUCE NITROGEN FERTILIZER REQUIREMENT OF LETTUCE
,
Acta Scientiarum Polonorum Hortorum Cultus: Vol. 19 No. 5 (2020)
-
Barbara Skwaryło-Bednarz,
Agnieszka Jamiołkowska,
Marek Kopacki,
Elżbieta Patkowska,
Katarzyna Golan,
Patrycja Krasowska,
Hanna Klikocka,
Assessment of catalase soil activity under amaranth cultivation not exposed to chemical protection methods
,
Acta Scientiarum Polonorum Hortorum Cultus: Vol. 21 No. 5 (2022)
-
Aysun Pekşen,
Beyhan Kibar,
DETERMINATION OF OPTIMUM CULTURE CONDITIONS FOR MYCELIAL GROWTH OF Macrolepiota procera MUSHROOM
,
Acta Scientiarum Polonorum Hortorum Cultus: Vol. 19 No. 1 (2020)
1 2 3 4 5 6 7 8 9 10 > >>
You may also start an advanced similarity search for this article.