Abstract
The concept to apply the bromocresole purple index (BCPI) as a new way for evaluation of neurotoxins activity in cooked chickling vetch seeds (determined as β-N-oxalyl-L-alanine – BOAA) was verified. Seeds of chickling vetch (Krab cv.) cooked under conditions of one of 10 intensity variants (heated independently in boiled distilled water for 5, 10, 15, 20, 25, 30, 35, 40, 45, and 50 min.) in reference to untreated sample, were study objects. Every sample was subjected to determination using BCPI and BOAA methods. Statistical processing of data revealed that BCPI and BOAA methods correspond to each other in testing of cooked chickling vetch seeds (correlation coefficient r = 86.34%), and both features may be associated with hypothetical functions of the form BOAA = f(BCPI). Their usefulness in analyses was statistically confirmed by achieving high fitting coefficients of the formulae to the real experimental data (R2 = 74.94% for power equation to 98.41% for 4th order polynomial equation).
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