The influence of exogenous gibberellic acid (GA3) and 24-epibrassinolide (24-EpiBL) on seed germination and the expression of genes involved in GA and BR synthesis/signalling in pepper (Capsicum annuum L.)

Selin Çayan

Ankara University, Faculty of Agriculture, Department of Horticulture, Ankara, Turkey

Gölge Sarıkamış

Ankara University Faculty of Agriculture Department of Horticulture

Canan Yüksel Özmen

Ankara University, Biotechnology Institute, Ankara, Turkey

Umut Kibar

Republic of Turkey, Ministry of Agriculture and Forestry, Agriculture and Rural Development Support Institution, Ankara, Turkey

Eren Özden

Iğdır University, Faculty of Agriculture, Department of Horticulture, Iğdır, Turkey

Ali Ergül

Ankara University, Biotechnology Institute, Ankara, Turkey


Abstract

Gibberellins (GAs) and brassinosteroids (BRs) are the plant hormones involved in various physiological processes including seed germination. In this study, the effects of exogenous gibberellic acid (GA3) and 24-epibrassinolide (24-EpiBL) treatments on the expression of key genes involved in GA and BR syntheis/signalling during seed germination were investigated in pepper (Capsicum annuum L).

The expressions of BES1 and BRI1 involved in BR synthesis/signalling pathway as well as GA3OX1 and GA20OX1 associated with gibberellic acid biosynthesis in plants were determined. Exogenous GA3 treatments increased BES1 expression and the highest increase was determined with 10⁻⁸ M BR + 100 µM GA3 (P<0.05).  On the contrary, the expression of BRI1 gene was significantly decreased by 10-8 M BR + 100 µM GA3 (P<0.05). The expression of GA3OX1 gene was induced with BR and GA3 treatments (P<0.05). GA20OX1 gene expression was generally higher compared to the expression of GA3OX1 and significantly increased by the GA3 treatments. Our findings are expected to bring an insight to the influence of BRs during seed germination together with the expression of associated genes.

Keywords:

brassinosteroids, Capsicum annuum, expression, germination, gibberellins, pepper

Bai, M.Y., Shang, J.X., Oh, E., Fan, M., Bai, Y., Zentella, R., Sun, T., Wang, Z.Y. (2012). Brassinosteroid, gibberellin and phytochrome impinge on a common transcription module in Arabidopsis. Nat. Cell Biol., 14, 810–817. https://doi.org/10.1038/ncb2546

Cheon, J., Fujioka, S., Dilkes, B.P., Choe, S. (2013). Brassinosteroids regulate plant growth through distinct signaling pathways in Selaginella and Arabidopsis. PlosOne, 8(12), e81938. https://doi.org/10.1371/journal.pone.0081938

Chung, Y., Choe, S. (2013). The regulation of brassinosteroid biosynthesis in Arabidopsis. Critic. Rev. Plant Sci., 32(6), 396–410. https://doi.org/10.1080/07352689.2013.797856

Davière, J.M., Achard, P. (2015). A pivotal role of DELLAs in regulating multiple hormone signals. Mol. Plant, 9(1), 10–20. https://doi.org/10.1016/j.molp.2015.09.011

Da Silva, C.B., Marcos-Filho, J., Jourdan, P., Bennett, M.A. (2015). Performance of bell pepper seeds in response to drum priming with addition of 24-epibrassinolide. HortSci., 50(6), 873–878. https://doi.org/10.21273/HORTSCI.50.6.873

Gudesblat, G.E., Russinova, E. (2011). Plants grow on brassinosteroids. Curr. Opin. Plant Biol., 14(5), 530–537. https://doi.org/10.1016/j.pbi.2011.05.004

Hategan, L., Godza, B., Kozma‑Bognar, L., Bishop, G.J., Szekeres, M. (2014). Differential expression of the brassinosteroid receptor‑encoding BRI1 gene in Arabidopsis. Planta, 239, 989–1001. https://doi.org/10.1007/s00425-014-2031-4

ISTA (2017). International rules for seed testing, International Seed Testing Association, Bassersdorf, Switzerland.

Kucera, B., Cohn, M.A., Leubner-Metzger, G. (2005). Plant hormone interactions during seed dormancy release and germination. Seed Sci. Res., 15(4), 281–307. https://doi.org/10.1079/SSR2005218

Li, J., Chory, J. (1997). A putative leucine rich repeat receptor kinase involved in brassinosteroid signal transduction. Cell, 90(5), 929–938. https://doi.org/10.1016/s0092-8674(00)80357-8

Li, Q.F., He, J.X. (2013). Mechanisms of signaling crosstalk between brassinosteroids and gibberellins. Plant Signal Behav., 8(7), e24686. https://doi.org/10.4161/psb.24686

Li, Q., Lu, J., Yu, J.W., Zhang, C.Q., He, J.X., Liu, Q.Q. (2018). The brassinosteroid-regulated transcription factors BZR1/BES1 function as a coordinator in multisignal-regulated plant growth. Biochim. Biophys. Acta Gene Regul. Mech., 1861(6), 561–571. https://doi.org/10.1016/j.bbagrm.2018.04.003

Livak, K.J., Schmittgen, T.D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods, 25, 402–408. https://doi.org/10.1006/meth.2001.1262

Ma, H.Y., Zhao, D.D., Ning, Q.R. Wei, J.P., Li, Y., Wang, M-M., Liu, X-L., Jiang, C-J., Liang, Z-W. (2018). A Multi-year beneficial effect of seed priming with gibberellic acid-3 (GA3) on plant growth and production in a perennial grass, Leymus chinensis. Sci. Rep., 8(1), 13214. https://doi:10.1038/s41598-018-31471-w

Miransari, M., Smith, D.L. (2014). Plant hormones and seed germination. Environ. Exp. Bot., 99, 110–121. https://doi.org/10.1016/j.envexpbot.2013.11.005

Mitchum, M.G., Yamaguchi, S., Hanada, A., Kuwahara, A., Yoshioka, Y., Kato, T., Tabata, S., Kamiya, Y., Sun, T-P. (2006). Distinct and overlapping roles of two gibberellin 3-oxidases in Arabidopsis development. Plant J., 45(5), 804–818. https://doi:10.1111/j.1365-313X.2005.02642.x

Ross, J.J., Quittenden, J.L. (2016). Interactions Between Brassinosteroids and Gibberellins: Synthesis or Signaling? Letter to the editor. Plant Cell, 28, 829–832. https://doi.org/10.1105/tpc.15.00917

Steber, C.M., McCourt, P. (2001). A role for brassinosteroids in germination in Arabidopsis. Plant Physiol., 125(2), 763–769. https://doi.org/10.1104/pp.125.2.763

Unterholzner, S.J., Rozhon, W., Papacek, M., Ciomas, J., Lange, T., Kugler, K.G., Mayer, K.F., Sieberer, T., Poppenberger, B. (2015). Brassinosteroids are master regulators of gibberellin biosynthesis in Arabidopsis. Plant Cell, 27(8), 2261–2272. https://doi.org/10.1105/tpc.15.00433

Qin, X., Liu, J.H., Zhao, W.S., Chen, X.J., Guo, Z.J., Peng, Y.L. (2013). Gibberellin 20-oxidase gene OsGA20OX3 regulates plant stature and disease development in rice. Mol. Plant Microbe Interact., 26(2), 227–323. https://doi.org/10.1094/MPMI-05-12-0138-R

Wang, W., Bai, M-Y., Wang, Z-Y. (2014).The brassinosteroid signalling network – a paradigm of signal integration. Curr. Opin. Plant Biol., 21, 147–153. https://doi.org/10.1016/j.pbi.2014.07.012

Wang, Z-Y., Nakano, T., Gendron, J., He, J., Chen, M., Vafeados, D., Yang, Y., Fujioka, S., Yoshida, S., Asami, T., Chory, J. (2002). Nuclear-localized BZR1 mediates brassinosteroid-induced growth and feedback suppression of brassinosteroid biosynthesis. Dev. Cell, 2(4), 505–513. https://doi.org/10.1016/S1534-5807(02)00153-3

Yamaguchi, S., Kamiya, Y. (2000). Gibberellin Biosynthesis: Its Regulation by Endogenous and Environmental Signals. Plant Cell Physiol., 41(3), 251–257. https://doi.org/10.1093/pcp/41.3.251

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Published
2021-10-29



Selin Çayan 
Ankara University, Faculty of Agriculture, Department of Horticulture, Ankara, Turkey
Gölge Sarıkamış 
Ankara University Faculty of Agriculture Department of Horticulture
Canan Yüksel Özmen 
Ankara University, Biotechnology Institute, Ankara, Turkey
Umut Kibar 
Republic of Turkey, Ministry of Agriculture and Forestry, Agriculture and Rural Development Support Institution, Ankara, Turkey
Eren Özden 
Iğdır University, Faculty of Agriculture, Department of Horticulture, Iğdır, Turkey
Ali Ergül 
Ankara University, Biotechnology Institute, Ankara, Turkey



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